a-21241 Search Results


a21240  (Jackson Immuno)
97
Jackson Immuno a21240
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
A21240, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Goat+Anti-Rat+IgG/pmc07044449-401-59-41
Average 97 stars, based on 1 article reviews
a21240 - by Bioz Stars, 2026-09
97/100 stars
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anti mouse igg1  (Vector Laboratories)
96
Vector Laboratories anti mouse igg1
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Anti Mouse Igg1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Mouse+IgG+(Control+Antibody)/pm40260844-150-18-34
Average 96 stars, based on 1 article reviews
anti mouse igg1 - by Bioz Stars, 2026-09
96/100 stars
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anti  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Anti, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Anti-rabbit+IgG+(H%2BL)%2C+F(ab')+2+Fragment/pmc09882292-208-17-20
Average 96 stars, based on 1 article reviews
anti - by Bioz Stars, 2026-09
96/100 stars
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horseradish peroxidase  (Thermo Fisher)
99
Thermo Fisher horseradish peroxidase
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Peroxidase%2C+horseradish/pmc08978607-51-14-24
Average 99 stars, based on 1 article reviews
horseradish peroxidase - by Bioz Stars, 2026-09
99/100 stars
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alexa fluor 647 conjugated goat anti rabbit igg  (Danaher Inc)
99
Danaher Inc alexa fluor 647 conjugated goat anti rabbit igg
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Alexa Fluor 647 Conjugated Goat Anti Rabbit Igg, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Goat+Anti-Rabbit+IgG+H%26L/pmc08652271-158-65-75
Average 99 stars, based on 1 article reviews
alexa fluor 647 conjugated goat anti rabbit igg - by Bioz Stars, 2026-09
99/100 stars
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cat a21271 rrid ab 221448 ihc  (Addgene inc)
90
Addgene inc cat a21271 rrid ab 221448 ihc
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Cat A21271 Rrid Ab 221448 Ihc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/ClpP-his+(Plasmid+%2322144)/10__7554_slash_elife__66998-254-89-115
Average 90 stars, based on 1 article reviews
cat a21271 rrid ab 221448 ihc - by Bioz Stars, 2026-09
90/100 stars
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alexa647 labelled secondary antibody  (Jackson Immuno)
96
Jackson Immuno alexa647 labelled secondary antibody
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Alexa647 Labelled Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Fluorescein+(FITC)+AffiniPure+Donkey+Anti-Rabbit+IgG/bio_rxiv__418988-183-16-22
Average 96 stars, based on 1 article reviews
alexa647 labelled secondary antibody - by Bioz Stars, 2026-09
96/100 stars
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anti mouse ifnar 1 in vivo  (Selleck Chemicals)
93
Selleck Chemicals anti mouse ifnar 1 in vivo
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Anti Mouse Ifnar 1 In Vivo, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Anti-mouse+NK1%2E1-InVivo/pm35468896-167-14-19
Average 93 stars, based on 1 article reviews
anti mouse ifnar 1 in vivo - by Bioz Stars, 2026-09
93/100 stars
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a21242  (Jackson Immuno)
96
Jackson Immuno a21242
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
A21242, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Peroxidase+AffiniPure+Goat+Anti-Mouse+IgG/pmc09582644__mbc___33___ar83___s001-21-124-129
Average 96 stars, based on 1 article reviews
a21242 - by Bioz Stars, 2026-09
96/100 stars
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a 21241 goat  (SouthernBiotech)
93
SouthernBiotech a 21241 goat
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
A 21241 Goat, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Mouse+IgG2b-APC/pm37428889-238-56-61
Average 93 stars, based on 1 article reviews
a 21241 goat - by Bioz Stars, 2026-09
93/100 stars
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alexa 647 igg  (Fisher Scientific)
86
Fisher Scientific alexa 647 igg
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Alexa 647 Igg, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/546+alexa/pm34425811-134-83-92
Average 86 stars, based on 1 article reviews
alexa 647 igg - by Bioz Stars, 2026-09
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goat anti rabbit alexa 647  (Jackson Immuno)
96
Jackson Immuno goat anti rabbit alexa 647
Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by <t>Pax7</t> (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).
Goat Anti Rabbit Alexa 647, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a-21241/Goat+Anti-Rabbit+Whole+Serum/pm26704722-171-24-34
Average 96 stars, based on 1 article reviews
goat anti rabbit alexa 647 - by Bioz Stars, 2026-09
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Image Search Results


Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by Pax7 (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).

Journal: Journal of Cell Science

Article Title: The gap junction protein connexin 43 controls multiple aspects of cranial neural crest cell development

doi: 10.1242/jcs.235440

Figure Lengend Snippet: Cx43 depletion decreases the number of premigratory neural crest cells in the absence of any changes in cell proliferation or cell death. Representative transverse sections taken through the midbrain and hindbrain of HH8+ to HH9 embryos after unilateral electroporation of the neural tube at HH7+ to HH8− (2ss–3ss) with either the control morpholino (MM) or Cx43 MO and immunostaining for various neural crest cell markers. In MM-treated embryos (A–A″,C–C″,E–E″,G–G″,I–I″,K–K″,M–M″), there is no decrease in the level of Cx43 in the neural tube (A′) and no change in any of the premigratory neural crest cells as identified by Pax7 (C′), Snail2 (E′), Sox10 (G′). Similarly, there are no changes in N-cadherin (I–I″), a marker of neural tube cells, cell proliferation [pHH3 (K–K″)]; or cell death [TUNEL (M–M″)]. In Cx43 MO-treated embryos (B–B″,D–D″,F–F″,H–H″,J–J″,L–L″,N–N″), however, there is obvious knockdown of Cx43 in the neural tube (B′, arrow) and also a reduction in the premigratory neural crest cell population observed after Pax7 (D′, arrow), Snail2 (F′, arrow) and Sox10 (H′, arrow) immunostaining. Interestingly, N-cadherin (J–J″) is not affected. Cx43 MO-mediated loss of Cx43 also does not seem to alter levels of either cell proliferation (L–L″) or cell death (N–N″). Additionally, in HH9 embryos, a small number of MO-positive neural crest cells begin to emerge from the neural tube (D′,F′,H′, arrowheads; neural tube indicated by dashed lines). Scale bars: 20 µm (A–K″,M–N″); 50 µm (L–L″).

Article Snippet: The following day, after washing four times for 30 min with 1× PBST, sections were incubated with appropriate secondary antibodies and incubated for 2–3 h at room temperature or overnight at 4°C [goat anti-rabbit IgG 488 or 647; Invitrogen, A11034 and Jackson ImmunoResearch, PA, USA; 111-605-003, respectively (for Cx43, Sox10, pHH3 and Snail2); goat anti-mouse IgG 1 647; Invitrogen, A21240 (for Pax7 and E-cadherin); goat anti-rat IgG; Life Technologies, MD; A21247 (for N-cadherin)].

Techniques: Electroporation, Control, Immunostaining, Marker, TUNEL Assay, Knockdown

Graphical representation of analyzed premigratory neural crest cell data after control or Cx43 MO treatment. Box and whisker plots showing the third quartile (Q3), median and first quartile (Q1) range of the data as well as data outliers. Whiskers represent minimum and maximum values. (A–C) Graphs depicting the number of premigratory, migratory and total neural crest cells (NCCs) electroporated with either the control MM or Cx43 MO normalized to the un-electroporated contralateral side for the analyzed neural crest cell markers Pax7 (A), Snail2 (B) and Sox10 (C). Asterisks denote statistically significant differences determined with un-paired Mann–Whitney (Wilcoxon) test that employed a Bonferroni correction [A, Cx43 MO premigratory NCCs (P=8.64×10−7); B, Cx43 MO premigratory NCCs (P=6.41×10−9), Cx43 MO migratory NCCs (P=0.0029), Cx43 MO Total NCCs (P=9.64×10−9); C, Cx43 MO Premigratory NCCs (P=0.0001), Cx43 MO Total NCCs (P=2.76×10−6)]. (D) Graph depicting the fluorescence intensity of N-cadherin in premigratory neural crest cells (NCCs) and the neural tube (NT) of control MM- (N=4) or Cx43 MO- (N=5) electroporated cells compared to the contralateral side.

Journal: Journal of Cell Science

Article Title: The gap junction protein connexin 43 controls multiple aspects of cranial neural crest cell development

doi: 10.1242/jcs.235440

Figure Lengend Snippet: Graphical representation of analyzed premigratory neural crest cell data after control or Cx43 MO treatment. Box and whisker plots showing the third quartile (Q3), median and first quartile (Q1) range of the data as well as data outliers. Whiskers represent minimum and maximum values. (A–C) Graphs depicting the number of premigratory, migratory and total neural crest cells (NCCs) electroporated with either the control MM or Cx43 MO normalized to the un-electroporated contralateral side for the analyzed neural crest cell markers Pax7 (A), Snail2 (B) and Sox10 (C). Asterisks denote statistically significant differences determined with un-paired Mann–Whitney (Wilcoxon) test that employed a Bonferroni correction [A, Cx43 MO premigratory NCCs (P=8.64×10−7); B, Cx43 MO premigratory NCCs (P=6.41×10−9), Cx43 MO migratory NCCs (P=0.0029), Cx43 MO Total NCCs (P=9.64×10−9); C, Cx43 MO Premigratory NCCs (P=0.0001), Cx43 MO Total NCCs (P=2.76×10−6)]. (D) Graph depicting the fluorescence intensity of N-cadherin in premigratory neural crest cells (NCCs) and the neural tube (NT) of control MM- (N=4) or Cx43 MO- (N=5) electroporated cells compared to the contralateral side.

Article Snippet: The following day, after washing four times for 30 min with 1× PBST, sections were incubated with appropriate secondary antibodies and incubated for 2–3 h at room temperature or overnight at 4°C [goat anti-rabbit IgG 488 or 647; Invitrogen, A11034 and Jackson ImmunoResearch, PA, USA; 111-605-003, respectively (for Cx43, Sox10, pHH3 and Snail2); goat anti-mouse IgG 1 647; Invitrogen, A21240 (for Pax7 and E-cadherin); goat anti-rat IgG; Life Technologies, MD; A21247 (for N-cadherin)].

Techniques: Control, Whisker Assay, MANN-WHITNEY, Fluorescence