Journal: Nature Communications

Article Title: A hippocampal circuit linking dorsal CA2 to ventral CA1 critical for social memory dynamics

doi: 10.1038/s41467-018-06501-w

Figure Lengend Snippet: dCA2 projections to vHPC are necessary for social memory. a AAV-DIO-hM4Di-mCherry was injected in dCA2 of Amigo2 -Cre mice and WT littermates. A cannula guide was implanted for local CNO or dye infusion in vCA1. b Ventral HPC horizontal slice showing overlap between dCA2 fibers located in vCA1a (labeled with hM4Di-mCherry, magenta) and dextran conjugated to Alexa Fluor 680 (green) infused in vCA1. c Ventral HPC coronal slice from an Amigo2 -Cre mouse after infusion in vHPC of a dextran conjugated to Alexa Fluor 488 (green). d Dorsal HPC coronal slice from same brain as in c , showing expression of hM4Di-mCherry (magenta) but no Alexa signal. e Intermediate HPC coronal slice showing lack of signal for hM4Di-mCherry or Alexa dye. f A direct interaction test was performed 30 min after bilateral local infusion of CNO in vHPC (2 µL of 1 mM solution per side). g WT mice displayed decreased social exploration time during trial 2 ( n = 6, P = 0.0110, Sidak’s multiple comparisons test) whereas hM4Di-mCherry expressing Amigo2 -Cre mice showed no decrease ( n = 9, P = 0.8351, Sidak’s multiple comparisons test). The two groups differed significantly (two-way ANOVA: treatment × trial F (1,13) = 4.964, P = 0.0442). h The percent reduction in interaction time in trial 2 versus trial 1 in Amigo2 -Cre mice was significantly less than in WT mice (unpaired t test: t (13) = 2.962, P = 0.0110). Results in g show mean ± s.e.m. Box-whiskers plot in h present median (center line), extension from the 25th to 75th percentiles (box) and minimal and maximal values (whiskers). * P < 0.05; ns, P > 0.05. Scale bar, 250 µm ( b , c , d , e )

Article Snippet: For RGS14 labeling, primary incubation was performed with mouse IgG2a antibody against RGS14 (1:500, UC Davis/NIH NeuroMab Facility, cat#75-170) and secondary incubation was performed with antibody conjugated to Alexa Fluor 488 (ThermoFisher, Scientific cat#A21131), Alexa Fluor 555 (ThermoFisher Scientific cat#A21137), or Alexa Fluor 647 (ThermoFisher Scientific, cat#A21241).

Techniques: Injection, Labeling, Expressing

Journal: PLOS Pathogens

Article Title: Isolation and characterization of IgG3 glycan-targeting antibodies with exceptional cross-reactivity for diverse viral families

doi: 10.1371/journal.ppat.1012499

Figure Lengend Snippet: (A) HEp-2 cell slides were immunofluorescently stained with the indicated mAbs and goat anti-human Ig-FITC secondary (green) and DAPI (blue, to stain cell nuclei). Each mAb was tested at 100, 10, and 1 μg/mL. Control human serum samples that were positive or negative for anti-nuclear antibodies are shown (right). Images are 40X magnification. (B) Autoantigens were tested for reactivity by 2526, Ab82 (negative control antibody), and A32 (positive control antibody) using a commercially available AtheNA Multi-Lyte ANA kit. Values shown in bold were considered positive.

Article Snippet: Antibody reactivity to nine human autoantigens was measured using the AtheNA Multi-Lyte ANA kit (Zeus scientific, Inc, #A21101).

Techniques: Staining, Control, Immunopeptidomics, Negative Control, Positive Control