Journal: Journal of Nanobiotechnology

Article Title: Design of RGD-functionalized GSH-responsive pegylated polymeric protacs for selective BRD4 degradation and EndMT-driven cardiac fibrosis inhibition

doi: 10.1186/s12951-026-04036-7

Figure Lengend Snippet: RGD-PEG-MZ1 exerted a profound inhibitory effect on the EndMT in vitro. ( A-D ) The expression of Collagen-I, Fibronectin, VE-cadherin, α-SMA and FSP1 was assessed using western blotting (WB) in HUVECs and HMEC-1. ( E ) Immunofluorescence staining for α-SMA (green) and VE-cadherin (green) in HUVECs. Scale bar, 100 μm. The data are presented as the Mean ± SD of three independent experiments. # p < 0.05 and ## p < 0.01 vs. NC group; ns, p > 0.05 vs. control group; * p < 0.05 and ** p < 0.01 vs. control group (one-way ANOVA followed by Bonferroni post hoc test)

Article Snippet: The slides were initially stabilized with a 5% goat serum solution, then exposed to primary antibodies—α-SMA (Proteintech, 14395-1-AP), Collagen-I (Proteintech, 14695-1-AP), CD31 (Proteintech, 66065-2-Ig), Raf1 (Abcam, ab181115), Raf1 (phospho S259) (Abcam, ab173539), ERK1 + ERK2 (Abcam, ab184699) and ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) (Abcam, ab278538)—and subsequently incubated at 4 °C for an extended period.

Techniques: In Vitro, Expressing, Western Blot, Immunofluorescence, Staining, Control

Journal: EMBO Molecular Medicine

Article Title: Characterization and therapy of fertilization failure in murine and human models with HNRNPR mutations

doi: 10.1038/s44321-026-00374-z

Figure Lengend Snippet: ( A ) MII oocyte and zygote fluorescence imaging reflecting fertilization after ICSI of Hnrnpr -mutated sperm. Formation of male and female pronuclei indicates successful fertilization. Tubulin labels the spindle, showing that the oocyte is at metaphase of meiosis II, and P1 marks the first polar body. The white dotted line defines the boundary of the zygotes. Scale bar = 10 μm. ( B ) Quantification of the percentage of zygotes from ( A ). Data are presented as mean ± s.d., and P values were calculated using a two-sided Student’s t test. Data in ( A , B ) represent results from six independent experiments (two technical and three biological replicates, n = 6).

Article Snippet: Rabbit anti-alpha Tubulin , MedChemExpress , Cat# HY- P86200.

Techniques: Fluorescence, Imaging

Journal: American Journal of Translational Research

Article Title: RNF128 suppresses the malignancy of colorectal cancer cells via inhibition of Wnt/β-catenin signaling

doi:

Figure Lengend Snippet: Loss of RNF128 increases β-catenin protein level and its target genes in colorectal cancer. (A) The mRNA expressions of cyclin D1, cyclin E1, cyclin A1 and cyclin B1 were quantified in RNF128 knockdown or overexpression cells compared to their respective controls in DLD1. (B) β-catenin, CCND1, and RNF128 expressions were confirmed using Western blotting. β-actin was used as a loading control. (C) Expression of β-catenin and RNF128 in nuclear and cytoplasmic fractions in RNF128-knockdown cells and control cells of DLD1. β-tubulin was used as the cytoplasmic reference protein. LaminB1 was used as the nuclear reference protein. *P<0.05, **P<0.01 and ***P<0.001. No significant difference (P>0.05, ns). Data represented as mean ± SD.

Article Snippet: Supernatants were collected for measuring protein concentration by BCA assay (Thermofisher Scientific, USA). β-tubulin Rabbit mAb, Lamin B1 Rabbit mAb and β-actin Rabbit mAb were purchased from Proteintech (china); β-Catenin Rabbit mAb and CCND1 Rabbit mAb were from Cell Signaling Technology (USA); Ub and RNF128 Rat mAb were from Santa Cruz Biotechnology (USA); goat anti-rabbit and goat anti-rat were from Cell Signaling Technology (USA).

Techniques: Over Expression, Western Blot, Expressing