RQ4649 Search Results


beta-2 microglobulin antibody  (NSJ Bioreagents)
94
NSJ Bioreagents beta-2 microglobulin antibody
Beta 2 Microglobulin Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/beta-2 microglobulin antibody/product/NSJ Bioreagents
Average 94 stars, based on 1 article reviews
beta-2 microglobulin antibody - by Bioz Stars, 2026-02
94/100 stars
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α cyp19a1  (NSJ Bioreagents)
94
NSJ Bioreagents α cyp19a1
Colonic epithelial cells express significant levels of ERα and ERβ. (A) Relative gene expression of Esr1 (ERα) and Esr2 (ERβ) in primary colonic epithelial cells obtained from healthy adult WT mice was determined by qPCR. Expression of target genes was normalized to that of Gapdh and fold change was calculated relative to male samples. (B) Ratio of Esr1:Esr2 mRNA expression was calculated for male and female samples. (C–H) Protein expression of ERα and ERβ were determined by western blot of nuclear (C–E) or cytoplasmic (F–H) protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice. Expression of ERα and ERβ were normalized to levels of Lamin B1 (nuclear lysates, [D]) or β-actin (cytoplasmic lysates, [G]) using densitometry. Densitometry values were used to calculate ratios of ERα:ERβ expressed in nucleus (E) and cytoplasm (H). Subcellular localization of ERα (I) and ERβ (J) was calculated using densitometry values. (K) Expression of <t>Cyp19A1</t> (aromatase) was determined by western blot of nuclear protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice and (L) quantified by densitometry. For all figures, statistical analysis was performed with 2-way analysis of variance and Tukey post hoc test. Individual points represent individual animals.
α Cyp19a1, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/α cyp19a1/product/NSJ Bioreagents
Average 94 stars, based on 1 article reviews
α cyp19a1 - by Bioz Stars, 2026-02
94/100 stars
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cdc7 antibody  (NSJ Bioreagents)
93
NSJ Bioreagents cdc7 antibody
Colonic epithelial cells express significant levels of ERα and ERβ. (A) Relative gene expression of Esr1 (ERα) and Esr2 (ERβ) in primary colonic epithelial cells obtained from healthy adult WT mice was determined by qPCR. Expression of target genes was normalized to that of Gapdh and fold change was calculated relative to male samples. (B) Ratio of Esr1:Esr2 mRNA expression was calculated for male and female samples. (C–H) Protein expression of ERα and ERβ were determined by western blot of nuclear (C–E) or cytoplasmic (F–H) protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice. Expression of ERα and ERβ were normalized to levels of Lamin B1 (nuclear lysates, [D]) or β-actin (cytoplasmic lysates, [G]) using densitometry. Densitometry values were used to calculate ratios of ERα:ERβ expressed in nucleus (E) and cytoplasm (H). Subcellular localization of ERα (I) and ERβ (J) was calculated using densitometry values. (K) Expression of <t>Cyp19A1</t> (aromatase) was determined by western blot of nuclear protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice and (L) quantified by densitometry. For all figures, statistical analysis was performed with 2-way analysis of variance and Tukey post hoc test. Individual points represent individual animals.
Cdc7 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdc7 antibody/product/NSJ Bioreagents
Average 93 stars, based on 1 article reviews
cdc7 antibody - by Bioz Stars, 2026-02
93/100 stars
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anti sucrase isomaltase  (NSJ Bioreagents)
89
NSJ Bioreagents anti sucrase isomaltase
Colonic epithelial cells express significant levels of ERα and ERβ. (A) Relative gene expression of Esr1 (ERα) and Esr2 (ERβ) in primary colonic epithelial cells obtained from healthy adult WT mice was determined by qPCR. Expression of target genes was normalized to that of Gapdh and fold change was calculated relative to male samples. (B) Ratio of Esr1:Esr2 mRNA expression was calculated for male and female samples. (C–H) Protein expression of ERα and ERβ were determined by western blot of nuclear (C–E) or cytoplasmic (F–H) protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice. Expression of ERα and ERβ were normalized to levels of Lamin B1 (nuclear lysates, [D]) or β-actin (cytoplasmic lysates, [G]) using densitometry. Densitometry values were used to calculate ratios of ERα:ERβ expressed in nucleus (E) and cytoplasm (H). Subcellular localization of ERα (I) and ERβ (J) was calculated using densitometry values. (K) Expression of <t>Cyp19A1</t> (aromatase) was determined by western blot of nuclear protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice and (L) quantified by densitometry. For all figures, statistical analysis was performed with 2-way analysis of variance and Tukey post hoc test. Individual points represent individual animals.
Anti Sucrase Isomaltase, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti sucrase isomaltase/product/NSJ Bioreagents
Average 89 stars, based on 1 article reviews
anti sucrase isomaltase - by Bioz Stars, 2026-02
89/100 stars
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igf2bp3 antibody  (NSJ Bioreagents)
N/A
RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter
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dis3 antibody / rrp44  (NSJ Bioreagents)
N/A
Exosome complex exonuclease RRP44, or Dis3, is an enzyme that in humans is encoded by the DIS3 gene. Its protein product is an RNase enzyme homologous to the yeast protein Rrp44, and can be part
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n-wasp antibody / neural wiskott-aldrich syndrome protein  (NSJ Bioreagents)
N/A
The WASL gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded
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thioredoxin antibody / trx / txn  (NSJ Bioreagents)
N/A
Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many
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mettl3 antibody / m6a  (NSJ Bioreagents)
N/A
N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by the METTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of
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lrh1 antibody / nr5a2  (NSJ Bioreagents)
N/A
The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression
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claudin 4 antibody / cldn4  (NSJ Bioreagents)
N/A
Claudin 4 plays a major role in tight junction-specific obliteration of the intercellular space.
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grancalcin antibody / gca  (NSJ Bioreagents)
N/A
Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to
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Image Search Results


Colonic epithelial cells express significant levels of ERα and ERβ. (A) Relative gene expression of Esr1 (ERα) and Esr2 (ERβ) in primary colonic epithelial cells obtained from healthy adult WT mice was determined by qPCR. Expression of target genes was normalized to that of Gapdh and fold change was calculated relative to male samples. (B) Ratio of Esr1:Esr2 mRNA expression was calculated for male and female samples. (C–H) Protein expression of ERα and ERβ were determined by western blot of nuclear (C–E) or cytoplasmic (F–H) protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice. Expression of ERα and ERβ were normalized to levels of Lamin B1 (nuclear lysates, [D]) or β-actin (cytoplasmic lysates, [G]) using densitometry. Densitometry values were used to calculate ratios of ERα:ERβ expressed in nucleus (E) and cytoplasm (H). Subcellular localization of ERα (I) and ERβ (J) was calculated using densitometry values. (K) Expression of Cyp19A1 (aromatase) was determined by western blot of nuclear protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice and (L) quantified by densitometry. For all figures, statistical analysis was performed with 2-way analysis of variance and Tukey post hoc test. Individual points represent individual animals.

Journal: Gastro Hep Advances

Article Title: Sex Differences in Colonic Inflammation are Driven by Epithelial-Specific Expression of Estrogen Receptor Alpha

doi: 10.1016/j.gastha.2025.100624

Figure Lengend Snippet: Colonic epithelial cells express significant levels of ERα and ERβ. (A) Relative gene expression of Esr1 (ERα) and Esr2 (ERβ) in primary colonic epithelial cells obtained from healthy adult WT mice was determined by qPCR. Expression of target genes was normalized to that of Gapdh and fold change was calculated relative to male samples. (B) Ratio of Esr1:Esr2 mRNA expression was calculated for male and female samples. (C–H) Protein expression of ERα and ERβ were determined by western blot of nuclear (C–E) or cytoplasmic (F–H) protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice. Expression of ERα and ERβ were normalized to levels of Lamin B1 (nuclear lysates, [D]) or β-actin (cytoplasmic lysates, [G]) using densitometry. Densitometry values were used to calculate ratios of ERα:ERβ expressed in nucleus (E) and cytoplasm (H). Subcellular localization of ERα (I) and ERβ (J) was calculated using densitometry values. (K) Expression of Cyp19A1 (aromatase) was determined by western blot of nuclear protein lysates isolated from primary colonic epithelial cells of healthy adult WT mice and (L) quantified by densitometry. For all figures, statistical analysis was performed with 2-way analysis of variance and Tukey post hoc test. Individual points represent individual animals.

Article Snippet: Antibodies used for western blots included α-Cyp19A1 (NSJ Bioreagents #RQ4643) α-ERα (Novus #NB300-560), α-ERβ (Novus #NB120-3577), α-GPER1 (Abcam #ab260033), α-Lamin B1 (Cell Signaling #12586), and α-β-Actin (Cell Signaling #12262).

Techniques: Gene Expression, Expressing, Western Blot, Isolation