Journal: eLife

Article Title: Reprogramming of bone marrow myeloid progenitor cells in patients with severe coronary artery disease

doi: 10.7554/eLife.60939

Figure Lengend Snippet:

Article Snippet: Circulating IL-1β, IL-1Ra, IL-6, and IL-18 concentrations were measured with the SimplePlex cartridge on the Ella platform (ProteinSimple, San Jose, CA).

Techniques: Isolation, Staining, Enzyme-linked Immunosorbent Assay, Sequencing, Recombinant, Software, Flow Cytometry, Gene Expression, Positron Emission Tomography-Computed Tomography

Journal: Oncotarget

Article Title: PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia

doi: 10.18632/oncotarget.7142

Figure Lengend Snippet: A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).

Article Snippet: Whole cell lysates were prepared in modified RIPA buffer [50 mM Tris-HCl (pH 7.5), 1 mM β-mercaptoethanol, 150 mM NaCl, 1% Igepal, and protease inhibitor cocktail] and incubated overnight with the following antibodies (catalog number and supplier): HIF-1α (sc-10790, Santa Cruz); V5 epitope (NB600-381, Novus Biologicals), HIF-2α (NB100-122, Novus Biologicals), or p300 (NB500-161, Novus Biologicals) in the presence of protein A Sepharose beads (NBP1-97240, Novus Biologicals).

Techniques: Transfection, Plasmid Preparation, Incubation, Purification, Western Blot, Staining