HY-W099503 Search Results


1 heptanol  (MedChemExpress)
93
MedChemExpress 1 heptanol
Dendritic cells siphon RNA from neighboring cells through a contact dependent mechanism that does not resemble conventional means of antigen uptake (A) Outline of experiment to measure RNA and protein transfer to MutuDC1s with or without direct contact. (B) Flow cytometric analysis of SYTO62 RNA signal measured in MutuDC1s after 45 min incubation with RNA labeled COCA KCs. Results are from a single experiment. (C) Representative images and quantification of SYTO62 signal contained within MutuDC1s after keratinocyte:DC co-cultures. Mean pixel intensity of far-red channel (SYTO62) was calculated within the area occupied by GFP+ MutuDC1s on a per cell basis. Images acquired with a Nikon A1R confocal microscope using a Plan Fluor 40× Oil objective. Dots represent individual cells. Results are from a single experiment. (D) MutuDC1s (green) interacting with COCA KCs. Max projections of z stack images taken on a Nikon A1R confocal microscope using a Plan Fluor 40× Oil objective plus 10x scanner zoom. (E) Transfer of SYTO62 labeled RNA to MutuDC1s from keratinocytes relative to vehicle controls in the presence of an ATPsynthase inhibitor (1 μM Oligomycin A), an inhibitor of F-actin formation (8 μM Cytochalasin D), a macropinocytosis inhibitor (32 μM 5-(N-Ethyl-N-isopropyl) amiloride (EIPA), and a gap junction inhibitor (5 mM <t>1-Heptanol).</t> Data normalized and pooled from three experiments. Data are represented as mean ± SD.
1 Heptanol, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 heptanol/product/MedChemExpress
Average 93 stars, based on 1 article reviews
1 heptanol - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


Dendritic cells siphon RNA from neighboring cells through a contact dependent mechanism that does not resemble conventional means of antigen uptake (A) Outline of experiment to measure RNA and protein transfer to MutuDC1s with or without direct contact. (B) Flow cytometric analysis of SYTO62 RNA signal measured in MutuDC1s after 45 min incubation with RNA labeled COCA KCs. Results are from a single experiment. (C) Representative images and quantification of SYTO62 signal contained within MutuDC1s after keratinocyte:DC co-cultures. Mean pixel intensity of far-red channel (SYTO62) was calculated within the area occupied by GFP+ MutuDC1s on a per cell basis. Images acquired with a Nikon A1R confocal microscope using a Plan Fluor 40× Oil objective. Dots represent individual cells. Results are from a single experiment. (D) MutuDC1s (green) interacting with COCA KCs. Max projections of z stack images taken on a Nikon A1R confocal microscope using a Plan Fluor 40× Oil objective plus 10x scanner zoom. (E) Transfer of SYTO62 labeled RNA to MutuDC1s from keratinocytes relative to vehicle controls in the presence of an ATPsynthase inhibitor (1 μM Oligomycin A), an inhibitor of F-actin formation (8 μM Cytochalasin D), a macropinocytosis inhibitor (32 μM 5-(N-Ethyl-N-isopropyl) amiloride (EIPA), and a gap junction inhibitor (5 mM 1-Heptanol). Data normalized and pooled from three experiments. Data are represented as mean ± SD.

Journal: iScience

Article Title: Dendritic cells overcome Cre/Lox induced gene deficiency by siphoning cytosolic material from surrounding cells

doi: 10.1016/j.isci.2024.109119

Figure Lengend Snippet: Dendritic cells siphon RNA from neighboring cells through a contact dependent mechanism that does not resemble conventional means of antigen uptake (A) Outline of experiment to measure RNA and protein transfer to MutuDC1s with or without direct contact. (B) Flow cytometric analysis of SYTO62 RNA signal measured in MutuDC1s after 45 min incubation with RNA labeled COCA KCs. Results are from a single experiment. (C) Representative images and quantification of SYTO62 signal contained within MutuDC1s after keratinocyte:DC co-cultures. Mean pixel intensity of far-red channel (SYTO62) was calculated within the area occupied by GFP+ MutuDC1s on a per cell basis. Images acquired with a Nikon A1R confocal microscope using a Plan Fluor 40× Oil objective. Dots represent individual cells. Results are from a single experiment. (D) MutuDC1s (green) interacting with COCA KCs. Max projections of z stack images taken on a Nikon A1R confocal microscope using a Plan Fluor 40× Oil objective plus 10x scanner zoom. (E) Transfer of SYTO62 labeled RNA to MutuDC1s from keratinocytes relative to vehicle controls in the presence of an ATPsynthase inhibitor (1 μM Oligomycin A), an inhibitor of F-actin formation (8 μM Cytochalasin D), a macropinocytosis inhibitor (32 μM 5-(N-Ethyl-N-isopropyl) amiloride (EIPA), and a gap junction inhibitor (5 mM 1-Heptanol). Data normalized and pooled from three experiments. Data are represented as mean ± SD.

Article Snippet: Reagents used in this study include: 8 μM Cytochalasin D (Millipore Sigma), 10 μM Oligomycin A (Selleck Chemicals), 32 μM 5-(N-Ethyl-N-isopropyl)amiloride (EIPA) (Sigma Aldrich), 5 mM 1-Heptanol (Fisher Scientific), 5 mM EDTA (Fisher Scientific), 2 μM Thapsigargin (Fisher Scientific), 50 μM BAPTA-AM (Fisher Scientific), 50 μM LY294002 (Tocris), 350 nM ADH-1 (MedChem Express), 500 μg/mL Polyguanylic acid (Sigma-Aldrich), 1 mg/mL RGD peptide (Selleck Chemicals), and BLT-1 (MedChemExpress).

Techniques: Incubation, Labeling, Microscopy