HY-RS06249 Search Results


hmox1 human pre designed sirna set a  (MedChemExpress)
94
MedChemExpress hmox1 human pre designed sirna set a
(A) Real-time PCR analysis of <t>HMOX1</t> mRNA expression in KLE cells treated with 2 µM AKT-100, 5 µM HO-3867, or 30 µM hemin for 24 hours. (B) Real-time PCR analysis of HMOX1 mRNA expression in Hec50co cells treated with 0.5 µM AKT-100, 3 µM HO-3867, or 20 µM hemin for 24 hours. (C) Immunoblotting and densitometric quantification of HMOX1 protein levels in KLE cells under the same treatment conditions. (D) Immunoblotting and densitometric quantification of HMOX1 protein levels in Hec50co cells under the same treatment conditions. Data are presented as mean ± SD. p < 0.05, p < 0.01, p < 0.001; one-way ANOVA followed by Dunnett’s multiple comparisons test.
Hmox1 Human Pre Designed Sirna Set A, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Real-time PCR analysis of HMOX1 mRNA expression in KLE cells treated with 2 µM AKT-100, 5 µM HO-3867, or 30 µM hemin for 24 hours. (B) Real-time PCR analysis of HMOX1 mRNA expression in Hec50co cells treated with 0.5 µM AKT-100, 3 µM HO-3867, or 20 µM hemin for 24 hours. (C) Immunoblotting and densitometric quantification of HMOX1 protein levels in KLE cells under the same treatment conditions. (D) Immunoblotting and densitometric quantification of HMOX1 protein levels in Hec50co cells under the same treatment conditions. Data are presented as mean ± SD. p < 0.05, p < 0.01, p < 0.001; one-way ANOVA followed by Dunnett’s multiple comparisons test.

Journal: bioRxiv

Article Title: Curcumin Analogues Trigger HMOX1-Mediated Ferroptosis to Halt Endometrial Cancer Growth

doi: 10.1101/2025.11.18.689080

Figure Lengend Snippet: (A) Real-time PCR analysis of HMOX1 mRNA expression in KLE cells treated with 2 µM AKT-100, 5 µM HO-3867, or 30 µM hemin for 24 hours. (B) Real-time PCR analysis of HMOX1 mRNA expression in Hec50co cells treated with 0.5 µM AKT-100, 3 µM HO-3867, or 20 µM hemin for 24 hours. (C) Immunoblotting and densitometric quantification of HMOX1 protein levels in KLE cells under the same treatment conditions. (D) Immunoblotting and densitometric quantification of HMOX1 protein levels in Hec50co cells under the same treatment conditions. Data are presented as mean ± SD. p < 0.05, p < 0.01, p < 0.001; one-way ANOVA followed by Dunnett’s multiple comparisons test.

Article Snippet: HMOX1 Human Pre-designed siRNA Set A (MedChemExpress, #HY-RS06249) was combined with DreamFect Gold Transfection Reagent (OzBiosciences, #DG81000) and incubated for 5 minutes at room temperature.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot

(A) Graphical illustration showing how novel curcumin analogues induce ferroptosis via heme oxygenase-1 (HMOX1) upregulation to suppress endometrial cancer growth. (B) FerroOrange staining assessing intracellular iron levels in KLE cells treated with 2 µM AKT-100 for 24 hours. (C) DCFH-DA staining measuring total ROS levels under the same treatment conditions. Data are presented as mean ± SD. p < 0.05, p < 0.01; unpaired t-test.

Journal: bioRxiv

Article Title: Curcumin Analogues Trigger HMOX1-Mediated Ferroptosis to Halt Endometrial Cancer Growth

doi: 10.1101/2025.11.18.689080

Figure Lengend Snippet: (A) Graphical illustration showing how novel curcumin analogues induce ferroptosis via heme oxygenase-1 (HMOX1) upregulation to suppress endometrial cancer growth. (B) FerroOrange staining assessing intracellular iron levels in KLE cells treated with 2 µM AKT-100 for 24 hours. (C) DCFH-DA staining measuring total ROS levels under the same treatment conditions. Data are presented as mean ± SD. p < 0.05, p < 0.01; unpaired t-test.

Article Snippet: HMOX1 Human Pre-designed siRNA Set A (MedChemExpress, #HY-RS06249) was combined with DreamFect Gold Transfection Reagent (OzBiosciences, #DG81000) and incubated for 5 minutes at room temperature.

Techniques: Analogues, Staining

CyQUANT cell proliferation assays of KLE cells treated with 2 µM AKT-100 in combination with: (A) 5 µM zinc protoporphyrin IX (ZnPP, HMOX1 inhibitor), (B) 50 nM liproxstatin-1 (ferroptosis inhibitor), or (C) 10 µM Z-VAD-FMK (pan-caspase/apoptosis inhibitor) for 72 hours. Data are presented as mean ± SD. p < 0.05, * p < 0.01, *** p < 0.0001; two-way ANOVA followed by Šídák’s multiple comparisons test.

Journal: bioRxiv

Article Title: Curcumin Analogues Trigger HMOX1-Mediated Ferroptosis to Halt Endometrial Cancer Growth

doi: 10.1101/2025.11.18.689080

Figure Lengend Snippet: CyQUANT cell proliferation assays of KLE cells treated with 2 µM AKT-100 in combination with: (A) 5 µM zinc protoporphyrin IX (ZnPP, HMOX1 inhibitor), (B) 50 nM liproxstatin-1 (ferroptosis inhibitor), or (C) 10 µM Z-VAD-FMK (pan-caspase/apoptosis inhibitor) for 72 hours. Data are presented as mean ± SD. p < 0.05, * p < 0.01, *** p < 0.0001; two-way ANOVA followed by Šídák’s multiple comparisons test.

Article Snippet: HMOX1 Human Pre-designed siRNA Set A (MedChemExpress, #HY-RS06249) was combined with DreamFect Gold Transfection Reagent (OzBiosciences, #DG81000) and incubated for 5 minutes at room temperature.

Techniques: CyQUANT Assay

(A) Immunoblotting and densitometric quantification of HMOX1 protein levels in KLE cells transfected with 100 nM negative control siRNA (siRNA-Neg) or HMOX-1–specific siRNA (siRNA-HMOX1) for 48 hours, followed by treatment with 2 µM AKT-100 for 24 hours. Data were analyzed using two-way ANOVA followed by Šídák’s multiple comparisons test. (B) CyQUANT cell proliferation assay of KLE cells transfected with 100 nM siRNA for 48 hours and then treated with 2 µM AKT-100 for 72 hours. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Representative images of colony formation in KLE cells transfected with 100 nM siRNA for 48 hours and treated with 2 µM AKT-100 for 24 hours. (D) Quantification of colony formation shown in (C). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SD. p < 0.05, * p < 0.01, ** p < 0.001; ns, not significant.

Journal: bioRxiv

Article Title: Curcumin Analogues Trigger HMOX1-Mediated Ferroptosis to Halt Endometrial Cancer Growth

doi: 10.1101/2025.11.18.689080

Figure Lengend Snippet: (A) Immunoblotting and densitometric quantification of HMOX1 protein levels in KLE cells transfected with 100 nM negative control siRNA (siRNA-Neg) or HMOX-1–specific siRNA (siRNA-HMOX1) for 48 hours, followed by treatment with 2 µM AKT-100 for 24 hours. Data were analyzed using two-way ANOVA followed by Šídák’s multiple comparisons test. (B) CyQUANT cell proliferation assay of KLE cells transfected with 100 nM siRNA for 48 hours and then treated with 2 µM AKT-100 for 72 hours. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Representative images of colony formation in KLE cells transfected with 100 nM siRNA for 48 hours and treated with 2 µM AKT-100 for 24 hours. (D) Quantification of colony formation shown in (C). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SD. p < 0.05, * p < 0.01, ** p < 0.001; ns, not significant.

Article Snippet: HMOX1 Human Pre-designed siRNA Set A (MedChemExpress, #HY-RS06249) was combined with DreamFect Gold Transfection Reagent (OzBiosciences, #DG81000) and incubated for 5 minutes at room temperature.

Techniques: Western Blot, Transfection, Negative Control, CyQUANT Assay, Proliferation Assay