HY-P99867 Search Results


anti tigit neutralizing antibody  (MedChemExpress)
94
MedChemExpress anti tigit neutralizing antibody
MM cells induce <t>TIGIT−mediated</t> ILC2 cell death: ( A ) Representative dot plots and relative statistical analysis showing expression of DNAM−1 and TIGIT on ILC2s from PB and BM of MM pts. Scatter plots indicate percentage ± SEM of TIGIT+ ILC2s. *** p < 0.001. ( n = 20). ( B ) Flow cytometry analysis showing steady-state expression of PD−1 and IL−10 production following PMA/Ionomycin stimulation on BM−MM ILC2s. Scatter plots indicate percentage ± SEM of IL−10-producing ILC2s. *** p < 0.001. ( n = 10). ( C ) Correlation between percentage of MM cells and TIGIT+ ILC2s in BM−MM ( n = 20). *** p < 0.001; R = 0.5. ( n = 20). ( D ) Graph represents daily expression of DNAM−1 on ILC2s from HDs and PB−MM pts following 5 days of culture with autologous MM cells. ( n = 10). ( E ) Graph represents daily expression of TIGIT on ILC2s from HDs and PB−MM pts following 5 days of culture with autologous MM cells. ( n = 10). ( F ) Representative dot plots and statistical analysis showing Annexin V expression on ILC2s from MM pts (PB and BM). Scatter plots indicate percentage ± SEM of Annexin V+ ILC2s from MM pts (PB and BM); *** p < 0.001. ( n = 20). ( G ) Representative dot plots and relative statistical analysis showing expression of 7−AAD and Annexin V on ILC2s from BM−MM pts following 48 h of culture with primary MM cells in presence of TIGIT−blocking mAb or IgG1 kappa isotype as control. Scatter plots indicate percentage ± SEM of death cells ( n = 10). *** p < 0.001. ( H ) Representative histogram showing expression of DNAM−1 on ILC2s from BM−MM pts following 48 h of culture with autologous MM cells in presence of TIGIT−blocking mAb or IgG1 kappa isotype as control. ( I ) Histograms showing MM cell viability following culture with ILC2s from BM−MM in presence or absence of TIGIT−blocking mAb or IgG1 kappa isotype as control. Scatter plots indicate percentage ± SEM of MM dead cells. ** p < 0.01. n.s., not significant; ( n = 10).
Anti Tigit Neutralizing Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MM cells induce TIGIT−mediated ILC2 cell death: ( A ) Representative dot plots and relative statistical analysis showing expression of DNAM−1 and TIGIT on ILC2s from PB and BM of MM pts. Scatter plots indicate percentage ± SEM of TIGIT+ ILC2s. *** p < 0.001. ( n = 20). ( B ) Flow cytometry analysis showing steady-state expression of PD−1 and IL−10 production following PMA/Ionomycin stimulation on BM−MM ILC2s. Scatter plots indicate percentage ± SEM of IL−10-producing ILC2s. *** p < 0.001. ( n = 10). ( C ) Correlation between percentage of MM cells and TIGIT+ ILC2s in BM−MM ( n = 20). *** p < 0.001; R = 0.5. ( n = 20). ( D ) Graph represents daily expression of DNAM−1 on ILC2s from HDs and PB−MM pts following 5 days of culture with autologous MM cells. ( n = 10). ( E ) Graph represents daily expression of TIGIT on ILC2s from HDs and PB−MM pts following 5 days of culture with autologous MM cells. ( n = 10). ( F ) Representative dot plots and statistical analysis showing Annexin V expression on ILC2s from MM pts (PB and BM). Scatter plots indicate percentage ± SEM of Annexin V+ ILC2s from MM pts (PB and BM); *** p < 0.001. ( n = 20). ( G ) Representative dot plots and relative statistical analysis showing expression of 7−AAD and Annexin V on ILC2s from BM−MM pts following 48 h of culture with primary MM cells in presence of TIGIT−blocking mAb or IgG1 kappa isotype as control. Scatter plots indicate percentage ± SEM of death cells ( n = 10). *** p < 0.001. ( H ) Representative histogram showing expression of DNAM−1 on ILC2s from BM−MM pts following 48 h of culture with autologous MM cells in presence of TIGIT−blocking mAb or IgG1 kappa isotype as control. ( I ) Histograms showing MM cell viability following culture with ILC2s from BM−MM in presence or absence of TIGIT−blocking mAb or IgG1 kappa isotype as control. Scatter plots indicate percentage ± SEM of MM dead cells. ** p < 0.01. n.s., not significant; ( n = 10).

Journal: Cancers

Article Title: Multiple Myeloma Cells Shift the Fate of Cytolytic ILC2s Towards TIGIT-Mediated Cell Death

doi: 10.3390/cancers17020263

Figure Lengend Snippet: MM cells induce TIGIT−mediated ILC2 cell death: ( A ) Representative dot plots and relative statistical analysis showing expression of DNAM−1 and TIGIT on ILC2s from PB and BM of MM pts. Scatter plots indicate percentage ± SEM of TIGIT+ ILC2s. *** p < 0.001. ( n = 20). ( B ) Flow cytometry analysis showing steady-state expression of PD−1 and IL−10 production following PMA/Ionomycin stimulation on BM−MM ILC2s. Scatter plots indicate percentage ± SEM of IL−10-producing ILC2s. *** p < 0.001. ( n = 10). ( C ) Correlation between percentage of MM cells and TIGIT+ ILC2s in BM−MM ( n = 20). *** p < 0.001; R = 0.5. ( n = 20). ( D ) Graph represents daily expression of DNAM−1 on ILC2s from HDs and PB−MM pts following 5 days of culture with autologous MM cells. ( n = 10). ( E ) Graph represents daily expression of TIGIT on ILC2s from HDs and PB−MM pts following 5 days of culture with autologous MM cells. ( n = 10). ( F ) Representative dot plots and statistical analysis showing Annexin V expression on ILC2s from MM pts (PB and BM). Scatter plots indicate percentage ± SEM of Annexin V+ ILC2s from MM pts (PB and BM); *** p < 0.001. ( n = 20). ( G ) Representative dot plots and relative statistical analysis showing expression of 7−AAD and Annexin V on ILC2s from BM−MM pts following 48 h of culture with primary MM cells in presence of TIGIT−blocking mAb or IgG1 kappa isotype as control. Scatter plots indicate percentage ± SEM of death cells ( n = 10). *** p < 0.001. ( H ) Representative histogram showing expression of DNAM−1 on ILC2s from BM−MM pts following 48 h of culture with autologous MM cells in presence of TIGIT−blocking mAb or IgG1 kappa isotype as control. ( I ) Histograms showing MM cell viability following culture with ILC2s from BM−MM in presence or absence of TIGIT−blocking mAb or IgG1 kappa isotype as control. Scatter plots indicate percentage ± SEM of MM dead cells. ** p < 0.01. n.s., not significant; ( n = 10).

Article Snippet: To examine MM cell death, tumor cells were co-cultured with or without ILC2s at an E:T ratio of 1:1 in the presence or absence of 5 μg/mL of anti-TIGIT neutralizing antibody (Tiragolumab Cat. No.: HY-P9986, MedChem Express, Monmouth Junction, NJ, USA) or corresponding human IgG1 kappa as an isotype control.

Techniques: Expressing, Flow Cytometry, Blocking Assay, Control

Features of MM-ILC2s along disease progression: ( A ) Representative dot plots showing frequency of GATA−3+ ILC2s in BM of MGUS ( n = 4), sMM ( n = 4) and MM pts ( n = 20). Scatter plot indicates percentage ± SEM of GATA-3+ ILC2s. ( B ) Representative dot plots showing expression of TIGIT on GATA−3+ ILC2s in BM during MM progression. Scatter plot represents percentage ±SEM of TIGIT+ ILC2s. ( C ) Intracellular expression of GZMB on GATA−3+ ILC2s in BM of MGUS ( n = 4), sMM ( n = 4) and MM pts ( n = 20). Scatter plot indicates percentage of GZMB+ ILC2s. Scatter plots: MGUS-pink; sMM-violet; MM-blue.

Journal: Cancers

Article Title: Multiple Myeloma Cells Shift the Fate of Cytolytic ILC2s Towards TIGIT-Mediated Cell Death

doi: 10.3390/cancers17020263

Figure Lengend Snippet: Features of MM-ILC2s along disease progression: ( A ) Representative dot plots showing frequency of GATA−3+ ILC2s in BM of MGUS ( n = 4), sMM ( n = 4) and MM pts ( n = 20). Scatter plot indicates percentage ± SEM of GATA-3+ ILC2s. ( B ) Representative dot plots showing expression of TIGIT on GATA−3+ ILC2s in BM during MM progression. Scatter plot represents percentage ±SEM of TIGIT+ ILC2s. ( C ) Intracellular expression of GZMB on GATA−3+ ILC2s in BM of MGUS ( n = 4), sMM ( n = 4) and MM pts ( n = 20). Scatter plot indicates percentage of GZMB+ ILC2s. Scatter plots: MGUS-pink; sMM-violet; MM-blue.

Article Snippet: To examine MM cell death, tumor cells were co-cultured with or without ILC2s at an E:T ratio of 1:1 in the presence or absence of 5 μg/mL of anti-TIGIT neutralizing antibody (Tiragolumab Cat. No.: HY-P9986, MedChem Express, Monmouth Junction, NJ, USA) or corresponding human IgG1 kappa as an isotype control.

Techniques: Expressing