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calnexin  (MedChemExpress)
93
MedChemExpress calnexin
Characterization of SAT and SAT-EVs. (A ) Schematic illustration of SAT-EVs extraction. ( B ) Subcutaneous adipose tissue was isolated from the abdominal region of mice. ( C ) NTA of SAT-EVs showing the size distribution and concentration. ( D ) TEM visualization showing the morphology of SAT-EVs (Scale bar = 100 nm). ( E ) WB analysis of SAT-EVs and SAT showing EV markers (Alix, <t>CD9,</t> <t>Tsg101)</t> and negative marker <t>(Calnexin).</t>
Calnexin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization of SAT and SAT-EVs. (A ) Schematic illustration of SAT-EVs extraction. ( B ) Subcutaneous adipose tissue was isolated from the abdominal region of mice. ( C ) NTA of SAT-EVs showing the size distribution and concentration. ( D ) TEM visualization showing the morphology of SAT-EVs (Scale bar = 100 nm). ( E ) WB analysis of SAT-EVs and SAT showing EV markers (Alix, CD9, Tsg101) and negative marker (Calnexin).

Journal: International Journal of Nanomedicine

Article Title: Delivery of miR-26a-5p by Subcutaneous Adipose Tissue-Derived Extracellular Vesicles Alleviates Acute Lung Injury in Mice Through CHUK/NF-κB Pathway

doi: 10.2147/IJN.S514623

Figure Lengend Snippet: Characterization of SAT and SAT-EVs. (A ) Schematic illustration of SAT-EVs extraction. ( B ) Subcutaneous adipose tissue was isolated from the abdominal region of mice. ( C ) NTA of SAT-EVs showing the size distribution and concentration. ( D ) TEM visualization showing the morphology of SAT-EVs (Scale bar = 100 nm). ( E ) WB analysis of SAT-EVs and SAT showing EV markers (Alix, CD9, Tsg101) and negative marker (Calnexin).

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies to ensure optimal binding to target proteins, including Chuk (1:2000, ET1611-15, HUABIO, China), CD9 (1:500, ab307885, Abcam, China), IL-1β (1:1000, 31202T, CST, US), TSG101 (1:2000, ab125011, Abcam, China), Calnexin (1:1000, 66,903-1-IgG, MCE, China), GAPDH (1:1000, GB15004, Servicebio, China), iNOS (1:1000, 18,985-1-AP, Proteintech, China), Phospho-p65 (1:1000, 3033T, CST, US), p65 (1:1000, 8242T, CST, US), IκBα (1:1000, 4014T, CST, US), Phospho-IκBα (1:1000, 2859T, CST, US), and PTGS2 (1:1000, 12282T, CST, US).

Techniques: Extraction, Isolation, Concentration Assay, Marker