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recombinant human cd47 protein  (MedChemExpress)
92
MedChemExpress recombinant human cd47 protein
Phototherapeutic effects and light‐controlled drug release of APHP‐CCCA. A) Time‐dependent inhibition of Cal33 cell viability by photothermal therapy using 100 µg mL −1 APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for different durations (5, 10, 15, and 20 min; NIR‐5, NIR‐10, NIR‐15, and NIR‐20). B) Quantitative analysis of intracellular PpIX concentration in Cal33 cells treated with 100 µg mL −1 APHP‐CCCA after different durations of 808 nm laser irradiation. C) Dose‐response curve from SPR measurements showing binding of aCD47 released from APHP‐CCCA without laser irradiation to immobilized <t>CD47</t> protein. D) Dose‐response curve from SPR measurements showing binding of aCD47 released from APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for 5 min to immobilized CD47 protein. E) SPR sensorgrams showing binding of free aCD47, aCD47 released from aCD47@CaCO 3 nanoparticles or APHP‐CCCA hydrogel after 808 nm laser irradiation for 5 min to immobilized CD47 protein. F) Dissociation equilibrium constant (K D ) values of aCD47 released from APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for different durations determined by SPR analysis. G) Quantitative analysis of intracellular PpIX concentration in Cal33 cells treated with APHP or APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 20 min followed by 24 h culture. H) Viability of Cal33 cells treated with APHP or APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 20 min followed by 24 h culture. I) ROS levels in Cal33 and HN6 cells determined by flow cytometry after treatment with 660 nm laser at 40 mW cm −2 for different durations (0, 2.5, 5, 7.5, and 10 min). J) Viability of Cal33 cells treated with APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min, followed by irradiation with 660 nm laser at 40 mW cm −2 for different durations (0, 2.5, 5, 7.5, and 10 min). K) Representative TEM images of the ultrastructure of Cal33 cells treated with APHP‐CCCA with no NIR irradiation, 5 min of 808 nm laser exposure, or 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Scale bar, 2 µm, 500 nm, and 100 nm. L) Intratumoral pH values on each day for 5 d following surgery. M) Representative fluorescence images of intracellular PpIX in residual tumors on each day for 5 d following surgery after 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Red, PpIX; blue, DAPI. Scale bar, 20 µm. N) Representative fluorescence images of CD47 expression in residual tumor tissue on each day for 5 d following surgery after 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Yellow, CD47; blue, DAPI. Scale bar, 20 µm. Data are presented as the mean ± SD; n = 3 independent experiments. p values were determined by two‐way ANOVA, Tukey's multiple‐comparison test (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001).
Recombinant Human Cd47 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Phototherapeutic effects and light‐controlled drug release of APHP‐CCCA. A) Time‐dependent inhibition of Cal33 cell viability by photothermal therapy using 100 µg mL −1 APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for different durations (5, 10, 15, and 20 min; NIR‐5, NIR‐10, NIR‐15, and NIR‐20). B) Quantitative analysis of intracellular PpIX concentration in Cal33 cells treated with 100 µg mL −1 APHP‐CCCA after different durations of 808 nm laser irradiation. C) Dose‐response curve from SPR measurements showing binding of aCD47 released from APHP‐CCCA without laser irradiation to immobilized CD47 protein. D) Dose‐response curve from SPR measurements showing binding of aCD47 released from APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for 5 min to immobilized CD47 protein. E) SPR sensorgrams showing binding of free aCD47, aCD47 released from aCD47@CaCO 3 nanoparticles or APHP‐CCCA hydrogel after 808 nm laser irradiation for 5 min to immobilized CD47 protein. F) Dissociation equilibrium constant (K D ) values of aCD47 released from APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for different durations determined by SPR analysis. G) Quantitative analysis of intracellular PpIX concentration in Cal33 cells treated with APHP or APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 20 min followed by 24 h culture. H) Viability of Cal33 cells treated with APHP or APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 20 min followed by 24 h culture. I) ROS levels in Cal33 and HN6 cells determined by flow cytometry after treatment with 660 nm laser at 40 mW cm −2 for different durations (0, 2.5, 5, 7.5, and 10 min). J) Viability of Cal33 cells treated with APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min, followed by irradiation with 660 nm laser at 40 mW cm −2 for different durations (0, 2.5, 5, 7.5, and 10 min). K) Representative TEM images of the ultrastructure of Cal33 cells treated with APHP‐CCCA with no NIR irradiation, 5 min of 808 nm laser exposure, or 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Scale bar, 2 µm, 500 nm, and 100 nm. L) Intratumoral pH values on each day for 5 d following surgery. M) Representative fluorescence images of intracellular PpIX in residual tumors on each day for 5 d following surgery after 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Red, PpIX; blue, DAPI. Scale bar, 20 µm. N) Representative fluorescence images of CD47 expression in residual tumor tissue on each day for 5 d following surgery after 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Yellow, CD47; blue, DAPI. Scale bar, 20 µm. Data are presented as the mean ± SD; n = 3 independent experiments. p values were determined by two‐way ANOVA, Tukey's multiple‐comparison test (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001).

Journal: Advanced Science

Article Title: Protecting Against Postsurgery Oral Cancer Recurrence with an Implantable Hydrogel Vaccine for In Situ Photoimmunotherapy

doi: 10.1002/advs.202309053

Figure Lengend Snippet: Phototherapeutic effects and light‐controlled drug release of APHP‐CCCA. A) Time‐dependent inhibition of Cal33 cell viability by photothermal therapy using 100 µg mL −1 APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for different durations (5, 10, 15, and 20 min; NIR‐5, NIR‐10, NIR‐15, and NIR‐20). B) Quantitative analysis of intracellular PpIX concentration in Cal33 cells treated with 100 µg mL −1 APHP‐CCCA after different durations of 808 nm laser irradiation. C) Dose‐response curve from SPR measurements showing binding of aCD47 released from APHP‐CCCA without laser irradiation to immobilized CD47 protein. D) Dose‐response curve from SPR measurements showing binding of aCD47 released from APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for 5 min to immobilized CD47 protein. E) SPR sensorgrams showing binding of free aCD47, aCD47 released from aCD47@CaCO 3 nanoparticles or APHP‐CCCA hydrogel after 808 nm laser irradiation for 5 min to immobilized CD47 protein. F) Dissociation equilibrium constant (K D ) values of aCD47 released from APHP‐CCCA upon 808 nm laser irradiation at 1.25 W cm −2 for different durations determined by SPR analysis. G) Quantitative analysis of intracellular PpIX concentration in Cal33 cells treated with APHP or APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 20 min followed by 24 h culture. H) Viability of Cal33 cells treated with APHP or APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 20 min followed by 24 h culture. I) ROS levels in Cal33 and HN6 cells determined by flow cytometry after treatment with 660 nm laser at 40 mW cm −2 for different durations (0, 2.5, 5, 7.5, and 10 min). J) Viability of Cal33 cells treated with APHP‐CCCA and irradiated with 808 nm laser at 1.25 W cm −2 for 5 min, followed by irradiation with 660 nm laser at 40 mW cm −2 for different durations (0, 2.5, 5, 7.5, and 10 min). K) Representative TEM images of the ultrastructure of Cal33 cells treated with APHP‐CCCA with no NIR irradiation, 5 min of 808 nm laser exposure, or 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Scale bar, 2 µm, 500 nm, and 100 nm. L) Intratumoral pH values on each day for 5 d following surgery. M) Representative fluorescence images of intracellular PpIX in residual tumors on each day for 5 d following surgery after 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Red, PpIX; blue, DAPI. Scale bar, 20 µm. N) Representative fluorescence images of CD47 expression in residual tumor tissue on each day for 5 d following surgery after 5 min of 808 nm plus 5 min of 660 nm laser irradiation. Yellow, CD47; blue, DAPI. Scale bar, 20 µm. Data are presented as the mean ± SD; n = 3 independent experiments. p values were determined by two‐way ANOVA, Tukey's multiple‐comparison test (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: Recombinant human CD47 protein was obtained from MedChemExpress (MCE, USA).

Techniques: Inhibition, Irradiation, Concentration Assay, Binding Assay, Flow Cytometry, Fluorescence, Expressing, Comparison

In vivo tumor recurrence suppression efficacy of APHP‐CCCA hydrogel implantation postsurgery.A) Schematic illustration of the animal experimental design. NIR irradiation: 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 5 min. B) Individual tumor growth curves of mice in different groups over 30 d following surgery. RR: recurrence rate. C) Average tumor growth curves of different groups during 30 d after surgery. Data are shown as mean ± SEM ( n = 5 mice per group). D) Percentage of mice with tumor recurrence in each group at 30 d after surgery ( n = 5 mice per group). E) Tumor weights of recurrent tumors on day 30 after surgery. Data are shown as mean ± SD ( n = 5 mice per group). F) Long‐term survival percentages of mice in each group over 60 d following surgery ( n = 5 mice per group). G) H&E staining of residual tumors in each group at 30 d after surgery; scale bar, 200 µm. Ki67 immunohistochemical staining of residual tumors in each group at third day after surgery; scale bar, 200 µm. Representative fluorescence images showing intracellular PpIX, CD47 expression, HSP90 expression and TUNEL in residual tumor tissue from each group at 30 d after surgery; scale bar, 200 µm. Blue: DAPI; red: PpIX; yellow: HSP90; green: TUNEL. H) Representative fluorescence images showing calcium influx and mitochondrial staining in live Cal33 cells from each group. Scale bar, 20 µm. Blue: DAPI; red: Rhod‐4 AM stain indicates calcium influx; green: MitoTracker Green FM stain indicates mitochondrial localization and membrane potential. Data are presented as the mean ± SD; n ≥ 3 independent experiments. p values were determined by two‐way ANOVA, Tukey's multiple‐comparison test (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001).

Journal: Advanced Science

Article Title: Protecting Against Postsurgery Oral Cancer Recurrence with an Implantable Hydrogel Vaccine for In Situ Photoimmunotherapy

doi: 10.1002/advs.202309053

Figure Lengend Snippet: In vivo tumor recurrence suppression efficacy of APHP‐CCCA hydrogel implantation postsurgery.A) Schematic illustration of the animal experimental design. NIR irradiation: 808 nm laser at 1.25 W cm −2 for 5 min and/or 660 nm laser at 40 mW cm −2 for 5 min. B) Individual tumor growth curves of mice in different groups over 30 d following surgery. RR: recurrence rate. C) Average tumor growth curves of different groups during 30 d after surgery. Data are shown as mean ± SEM ( n = 5 mice per group). D) Percentage of mice with tumor recurrence in each group at 30 d after surgery ( n = 5 mice per group). E) Tumor weights of recurrent tumors on day 30 after surgery. Data are shown as mean ± SD ( n = 5 mice per group). F) Long‐term survival percentages of mice in each group over 60 d following surgery ( n = 5 mice per group). G) H&E staining of residual tumors in each group at 30 d after surgery; scale bar, 200 µm. Ki67 immunohistochemical staining of residual tumors in each group at third day after surgery; scale bar, 200 µm. Representative fluorescence images showing intracellular PpIX, CD47 expression, HSP90 expression and TUNEL in residual tumor tissue from each group at 30 d after surgery; scale bar, 200 µm. Blue: DAPI; red: PpIX; yellow: HSP90; green: TUNEL. H) Representative fluorescence images showing calcium influx and mitochondrial staining in live Cal33 cells from each group. Scale bar, 20 µm. Blue: DAPI; red: Rhod‐4 AM stain indicates calcium influx; green: MitoTracker Green FM stain indicates mitochondrial localization and membrane potential. Data are presented as the mean ± SD; n ≥ 3 independent experiments. p values were determined by two‐way ANOVA, Tukey's multiple‐comparison test (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: Recombinant human CD47 protein was obtained from MedChemExpress (MCE, USA).

Techniques: In Vivo, Irradiation, Staining, Immunohistochemical staining, Fluorescence, Expressing, TUNEL Assay, Membrane, Comparison