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MedChemExpress adam
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MedChemExpress adam12
The Primers Used in This Study for RT-PCR
Adam12, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress acetylcholinesterase
The Primers Used in This Study for RT-PCR
Acetylcholinesterase, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The Primers Used in This Study for RT-PCR

Journal: OncoTargets and therapy

Article Title: SP1-Mediated Upregulation of lncRNA LINC01614 Functions a ceRNA for miR-383 to Facilitate Glioma Progression Through Regulation of ADAM12

doi: 10.2147/OTT.S242854

Figure Lengend Snippet: The Primers Used in This Study for RT-PCR

Article Snippet: The qPCR detection for SP1, LINC01614 and ADAM12 was conducted by using SYBR Green qPCR kits (MCE, Hangzhou, Zhejiang, China) according to the kits’ protocols.

Techniques:

LINC01614 abrogated the miR-383-mediated suppression on ADAM12. ( A ) Venn diagram. ( B ) Overall survival curves were evaluated by GEPIA algorithm. ( C ) cBioPortal algorithm analyzed the genetic alternation of related genes. ( D ) GEPIA algorithm analyzed ADAM12 expression in glioma samples from TCGA data. ( E ) qPCR determined ADAM12 levels in glioma specimens. ( F ) UALCAN algorithm analyzed ADAM12 expression across diverse cancer types. ( G ) GO and KEGG pathway analysis of ADAM12 positively correlated genes in glioma. ( H ) The predicted binding sites for miR-383 in ADAM12 mRNA 3ʹ-UTR. ( I ) Luciferase reporter assays. ( J ) qPCR determined LINC01614 and ADAM12 levels. ( K ) qPCR measured ADAM12 levels. ( L ) Western blot examined LINC01614 levels. **P < 0.01. n.s (not significant) represents “P > 0.05”.

Journal: OncoTargets and therapy

Article Title: SP1-Mediated Upregulation of lncRNA LINC01614 Functions a ceRNA for miR-383 to Facilitate Glioma Progression Through Regulation of ADAM12

doi: 10.2147/OTT.S242854

Figure Lengend Snippet: LINC01614 abrogated the miR-383-mediated suppression on ADAM12. ( A ) Venn diagram. ( B ) Overall survival curves were evaluated by GEPIA algorithm. ( C ) cBioPortal algorithm analyzed the genetic alternation of related genes. ( D ) GEPIA algorithm analyzed ADAM12 expression in glioma samples from TCGA data. ( E ) qPCR determined ADAM12 levels in glioma specimens. ( F ) UALCAN algorithm analyzed ADAM12 expression across diverse cancer types. ( G ) GO and KEGG pathway analysis of ADAM12 positively correlated genes in glioma. ( H ) The predicted binding sites for miR-383 in ADAM12 mRNA 3ʹ-UTR. ( I ) Luciferase reporter assays. ( J ) qPCR determined LINC01614 and ADAM12 levels. ( K ) qPCR measured ADAM12 levels. ( L ) Western blot examined LINC01614 levels. **P < 0.01. n.s (not significant) represents “P > 0.05”.

Article Snippet: The qPCR detection for SP1, LINC01614 and ADAM12 was conducted by using SYBR Green qPCR kits (MCE, Hangzhou, Zhejiang, China) according to the kits’ protocols.

Techniques: Expressing, Binding Assay, Luciferase, Western Blot