Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 5. Transcriptomics analysis, molecular docking, molecular dynamics simulation, and SPR analysis. A Volcano map of the 147 intersecting genes. B PPI network. C Kegg pathway. D Schematic diagram of molecular docking of 13-Me-PLT with ITGA5. E RMSD of ITGA5 with 13-Me-PLT. F Spacing of ITGA5 and 13-Me-PLT binding sites (Dock site-ligand). G Interaction of ITGA5 with 13-Me-PLT. H SPR analysis of ITGA5 and 13- Me-PLT.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Binding Assay

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 6. Effects of 13-Me-PLT on BLM-induced ITGA5 and TGF-β/Smad signaling pathways in mice and TGF-β/Smad signaling pathways in Ang II-stimulated MRC5 cells. A, B Immunohistochemical staining of ITGA5 in lung tissue and relative staining intensity analysis. Scale bar = 50 μm; n = 3. C, D mRNA levels of ITGA5 and TGF-β1 in lung tissue (n = 5–6). E-H Representative blotting and quantitative analysis of protein levels of ITGA5, TGF-β1 and p-Smad3 in lung tissue (n = 6). I, J Immu nofluorescence staining and relative fluorescence intensity analysis of ITGA5 in lung tissue. Scale bar = 100 μm; n = 3. K, l mRNA levels of ITGA5 and TGF-β1 in cells of each group (n = 4–5). M-O Quantitative analysis of protein levels of ITGA5, TGF-β1 and p-Smad3 in cells of each group (n = 4). P, Q Relative fluorescence intensity analysis of ITGA5 in cells of each group (n = 3). Data are expressed as SEM ± mean. **p < 0.01, ***p < 0.001 vs. Control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. BLM or AngII.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Protein-Protein interactions, Immunohistochemical staining, Staining, Fluorescence, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 7. Effects of silencing ITGA5 on fibrosis-related factors and the TGF-β/Smad signaling pathway in each group of cells. A-E mRNA levels of FN1, Collagen I, α-SMA, ITGA5, and TGF-β1 in cells of each group (n = 4). F-H Representative blotting and quantitative analysis of FN1 and Collagen I protein levels in cells of each group (n = 4). I-l Representative blotting and quantitative analysis of protein levels of ITGA5, TGF-β1 and p-Smad3 in each group of cells (n = 4). M, N Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of α-SMA in each group of cells (n = 3). Scale bar = 100 μm. O, P Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of ITGA5 in each group of cells (n = 3), scale bar = 100 μm. Q, R Transwell detection of cell migration numbers in each group of cells and statistical analysis of migration data. Scale bar = 75 μm; n = 3. Data are expressed as SEM ± mean. **p < 0.01,***p < 0.001 vs. Control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. AngII.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Immunofluorescence, Staining, Fluorescence, Migration, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 8. Effect of 13-Me-PLT on fibrosis-related indices and TGF-β/Smad signaling pathway in various groups of cells after overexpression of ITGA5. A-E mRNA levels of FN1, Collagen I, α-SMA, ITGA5, and TGF-β1 in cells of each group (n = 4). F-H Representative blotting and quantitative analysis of FN1 and Collagen I protein levels in cells of each group (n = 4). I-l Representative blotting and quantitative analysis of protein levels of ITGA5, TGF-β1 and p-smad3 in each group of cells (n = 4). M, N Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of α-SMA in each group of cells (n = 3). Scale bar = 100 μm. O, P Immunofluorescence staining and quantitative analysis of mean fluorescence intensity of α-SMA in each group of cells (n = 3). Scale bar = 100 μm. Q, R Transwell detection of cell migration numbers in each group of cells and statistical analysis of migration data (n = 3). Scale bar = 75 μm. Data are expressed as SEM ± mean. **p < 0.01,***p < 0.001 vs. Control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. P-ITGA5.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Over Expression, Immunofluorescence, Staining, Fluorescence, Migration, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: 13-Methylpalmatine alleviates bleomycin-induced pulmonary fibrosis by suppressing the ITGA5/TGF-β/Smad signaling pathway.

doi: 10.1016/j.phymed.2025.156545

Figure Lengend Snippet: Fig. 9. Schematic representation of possible targets and molecular mechanisms of 13-Me-PLT for the treatment of IPF. 13-Me-PLT ameliorates pulmonary fibrosis by inhibiting ITGA5, suppressing activation of the TGF-β/Smad signaling pathway, and decreasing phosphorylation of samd3, which reduces the deposition of ECM (collagens, fibronectin, α-SMA, etc.). 13-Me-PLT: 13-methylpalmatine; ITGA5: integrin alpha-5; ECM : extracellular matrix; TGF-β1: transforming growth factor-β1; TβR-I: Transforming Growth Factor Beta-Receptor Type I; TβR-II: Transforming Growth Factor Beta-Receptor Type II.

Article Snippet: The CM7 chip was selected and ITGA5 protein (50μg, shanghai, MCE) was diluted to 50 μg/ml using sodium acetate with a pH value of 4.0 and the protein was coupled to the chip surface (10,988 RU).

Techniques: Activation Assay, Phospho-proteomics