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thbs1  (MedChemExpress)
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Proliferation- and apoptosis-related gene detection in CL23 cells and M60 cells. ( a ) Proliferation- and apoptosis-related genes were expressed at the mRNA level in CL23 cells and M60 cells. ( b ) Proliferation- and apopto sis-related genes were expressed at the protein level in CL23 cells and M60 cells. ( c ) <t>THBS1</t> and EPHB2 protein level expression in CL23 cells and tumourigenic M60 cells. Differential grey value analysis. * indicates statistically significant difference (*** p < 0.001) and no * indicates no difference.
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Proliferation- and apoptosis-related gene detection in CL23 cells and M60 cells. ( a ) Proliferation- and apoptosis-related genes were expressed at the mRNA level in CL23 cells and M60 cells. ( b ) Proliferation- and apopto sis-related genes were expressed at the protein level in CL23 cells and M60 cells. ( c ) THBS1 and EPHB2 protein level expression in CL23 cells and tumourigenic M60 cells. Differential grey value analysis. * indicates statistically significant difference (*** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Proliferation- and apoptosis-related gene detection in CL23 cells and M60 cells. ( a ) Proliferation- and apoptosis-related genes were expressed at the mRNA level in CL23 cells and M60 cells. ( b ) Proliferation- and apopto sis-related genes were expressed at the protein level in CL23 cells and M60 cells. ( c ) THBS1 and EPHB2 protein level expression in CL23 cells and tumourigenic M60 cells. Differential grey value analysis. * indicates statistically significant difference (*** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Expressing

Construction and characterisation of MDCK cell lines stably knocking down and overexpressing THBS1 . ( a ) Bright field and fluorescence expression of cells after puromycin screening of knockdown control cells THBS1 -sh-con puromycin; bright field and fluorescence expression of cells after puromycin screening of knockdown cells THBS1 -sh-122484 locus; knockdown cells THBS1-sh-122485 locus puromycin screened for cell bright field and fluorescence expression; knockdown cells THBS1 -sh-122486 locus puromycin screened for cell bright field and fluorescence expression; overexpression of control cells THBS1 -OE-con puromycin screened for cell bright field and fluorescence expression; overexpression of THBS1 -OE cells’ bright field and fluorescence expression after puromycin screening. ( b ) Differential expression of THBS1 mRNA levels in stable knockdown THBS1 cells. ( c ) Differential expression of THBS1 mRNA levels in stable overexpression THBS1 cells. ( d ) Differential expression of THBS1 protein levels in stable knockdown and overexpression THBS1 cells. ( e , f ) Differential expression of THBS1 protein levels in stable knockdown and overexpression cells. Grey scale value analysis of THBS1 protein level expression differences in THBS1 cells. * indicates statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Construction and characterisation of MDCK cell lines stably knocking down and overexpressing THBS1 . ( a ) Bright field and fluorescence expression of cells after puromycin screening of knockdown control cells THBS1 -sh-con puromycin; bright field and fluorescence expression of cells after puromycin screening of knockdown cells THBS1 -sh-122484 locus; knockdown cells THBS1-sh-122485 locus puromycin screened for cell bright field and fluorescence expression; knockdown cells THBS1 -sh-122486 locus puromycin screened for cell bright field and fluorescence expression; overexpression of control cells THBS1 -OE-con puromycin screened for cell bright field and fluorescence expression; overexpression of THBS1 -OE cells’ bright field and fluorescence expression after puromycin screening. ( b ) Differential expression of THBS1 mRNA levels in stable knockdown THBS1 cells. ( c ) Differential expression of THBS1 mRNA levels in stable overexpression THBS1 cells. ( d ) Differential expression of THBS1 protein levels in stable knockdown and overexpression THBS1 cells. ( e , f ) Differential expression of THBS1 protein levels in stable knockdown and overexpression cells. Grey scale value analysis of THBS1 protein level expression differences in THBS1 cells. * indicates statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Stable Transfection, Fluorescence, Expressing, Knockdown, Control, Over Expression, Quantitative Proteomics

Effects of knockdown of THBS1 on proliferation, apoptosis, migration, and cell cycle of CL23 cells. ( a ) Growth curves of stably knocked down THBS1 cell lines. ( b ) Analysis of migratory ability of stably knocked down THBS1 cell lines. ( c ) Analysis of apoptotic ability of stably knocked down THBS1 cell lines. ( d ) Analysis of cell cycle of stably knocked down THBS1 cell lines. * denotes statistically significant difference (* p < 0.05; ** p < 0.01; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Effects of knockdown of THBS1 on proliferation, apoptosis, migration, and cell cycle of CL23 cells. ( a ) Growth curves of stably knocked down THBS1 cell lines. ( b ) Analysis of migratory ability of stably knocked down THBS1 cell lines. ( c ) Analysis of apoptotic ability of stably knocked down THBS1 cell lines. ( d ) Analysis of cell cycle of stably knocked down THBS1 cell lines. * denotes statistically significant difference (* p < 0.05; ** p < 0.01; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Knockdown, Migration, Stable Transfection

Effects of overexpression of THBS1 on proliferation, apoptosis, migration, and cell cycle of M60 cells. ( a ) Growth curves of stable overexpression of THBS1 cell lines. ( b ) Analysis of migration ability of stable overexpression of THBS1 cell lines. ( c ) Analysis of apoptosis ability of stable overexpression of THBS1 cell lines. ( d ) Analysis of cell cycle of stable overexpression of THBS1 cell lines. * denotes statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Effects of overexpression of THBS1 on proliferation, apoptosis, migration, and cell cycle of M60 cells. ( a ) Growth curves of stable overexpression of THBS1 cell lines. ( b ) Analysis of migration ability of stable overexpression of THBS1 cell lines. ( c ) Analysis of apoptosis ability of stable overexpression of THBS1 cell lines. ( d ) Analysis of cell cycle of stable overexpression of THBS1 cell lines. * denotes statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Over Expression, Migration

Effect of knockdown and overexpression of THBS1 on H1N1 influenza virus replication in MDCK cells. ( a , b ) Differences in expression of NP and NS1 mRNA levels across time of H1N1 influenza virus infection in stably knocked down THBS1 cell lines. ( c , d ) Differences in expression of NP protein levels across time of H1N1 influenza virus infection in stably knocked down THBS1 cell lines. ( e , f ) Stable overexpression of THBS1 cell lines infected with H1N1 influenza virus different time period NP and NS1 mRNA level expression differences. ( g , h ) Stable overexpression of THBS1 cell lines infected with H1N1 influenza virus different time period NP protein level expression differences. * indicates statistically significant difference (* p < 0.05; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Effect of knockdown and overexpression of THBS1 on H1N1 influenza virus replication in MDCK cells. ( a , b ) Differences in expression of NP and NS1 mRNA levels across time of H1N1 influenza virus infection in stably knocked down THBS1 cell lines. ( c , d ) Differences in expression of NP protein levels across time of H1N1 influenza virus infection in stably knocked down THBS1 cell lines. ( e , f ) Stable overexpression of THBS1 cell lines infected with H1N1 influenza virus different time period NP and NS1 mRNA level expression differences. ( g , h ) Stable overexpression of THBS1 cell lines infected with H1N1 influenza virus different time period NP protein level expression differences. * indicates statistically significant difference (* p < 0.05; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Knockdown, Over Expression, Virus, Expressing, Infection, Stable Transfection

Differential expression of target genes downstream of PI3K/Akt , P53 , and TGF-β/Smad signalling pathways, as well as the predicted THBS1-interacting gene, SCARB2 , in M60 and CL23 cells. ( a , b ) Differential expression of target genes downstream of the PI3K/Akt , P53 , and TGF-β/Smad signalling pathways, as well as SCARB2 at mRNA level. ( c , d ) Differential expression of target genes downstream of the TGF-β/Smad signalling pathway and SCARB2 at the protein level. * indicates statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Differential expression of target genes downstream of PI3K/Akt , P53 , and TGF-β/Smad signalling pathways, as well as the predicted THBS1-interacting gene, SCARB2 , in M60 and CL23 cells. ( a , b ) Differential expression of target genes downstream of the PI3K/Akt , P53 , and TGF-β/Smad signalling pathways, as well as SCARB2 at mRNA level. ( c , d ) Differential expression of target genes downstream of the TGF-β/Smad signalling pathway and SCARB2 at the protein level. * indicates statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Quantitative Proteomics

Effects of knockdown and overexpression of THBS1 on the differential expression of target genes downstream of TGF-β/Smad , PI3K/Akt , P53 signalling pathways, and SCARB2 in MDCK cells. ( a , b ) Effects of knockdown of THBS1 on the differential expression of target genes downstream of PI3K/Akt , P53 , TGF-β/Smad signalling, and the SCARB2 mRNA level expression differences in CL23 cells. ( c , d ) Differential effects of overexpression of THBS1 on the expression of target genes downstream of PI3K/Akt , P53 , TGF-β/Smad signalling, and SCARB2 at the mRNA level in M60 cells. ( e ) Differential effects of knockdown and overexpression of THBS1 on the expression of target genes downstream of the PI3K/Akt , P53 , TGF-β/Smad signalling pathways in MDCK cells, and the SCARB2 protein level expression differences. ( f , g ) Grey value analysis of knockdown and overexpression of THBS1 on the expression of target genes downstream of the PI3K/Akt , P53 , and TGF-β/Smad signalling pathways, as well as SCARB2 protein level in MDCK cells. * indicates statistically significant difference (* p < 0.05; ** p < 0.01; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Effects of knockdown and overexpression of THBS1 on the differential expression of target genes downstream of TGF-β/Smad , PI3K/Akt , P53 signalling pathways, and SCARB2 in MDCK cells. ( a , b ) Effects of knockdown of THBS1 on the differential expression of target genes downstream of PI3K/Akt , P53 , TGF-β/Smad signalling, and the SCARB2 mRNA level expression differences in CL23 cells. ( c , d ) Differential effects of overexpression of THBS1 on the expression of target genes downstream of PI3K/Akt , P53 , TGF-β/Smad signalling, and SCARB2 at the mRNA level in M60 cells. ( e ) Differential effects of knockdown and overexpression of THBS1 on the expression of target genes downstream of the PI3K/Akt , P53 , TGF-β/Smad signalling pathways in MDCK cells, and the SCARB2 protein level expression differences. ( f , g ) Grey value analysis of knockdown and overexpression of THBS1 on the expression of target genes downstream of the PI3K/Akt , P53 , and TGF-β/Smad signalling pathways, as well as SCARB2 protein level in MDCK cells. * indicates statistically significant difference (* p < 0.05; ** p < 0.01; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Knockdown, Over Expression, Quantitative Proteomics, Expressing

Determination of optimal concentration of TGF-β activator SRI-011381 on THBS1 -sh-122484 cells and inhibitor LY2109761 on THBS1 -OE cells, and its effect on the expression of target genes, THBS1 , downstream of TGF-β/Smad signalling. ( a ) Effect of different concentrations of SRI-011381 on the growth status of THBS1 -sh-122484 cells. ( b ) Effects of different concentrations of LY2109761 on the growth status of THBS1 -OE cells. ( c ) Effects of different concentrations of SRI-011381 on the differential expression of target genes downstream of TGF-β/Smad signalling and THBS1 mRNA levels in THBS1 -sh-122484 cells. ( d ) Effects of different concentrations of LY2109761 on the expression of target genes downstream of TGF-β/Smad signalling and THBS1 , and effects of different concentrations of LY2109761 on the expression of target genes downstream of THBS1 -Sh-122484 cells. LY2109761 on THBS1 -OE cells TGF-β/Smad signalling downstream target genes and THBS1 mRNA level expression differences. ( e ) Effect of different concentrations of SRI-011381 on THBS1 -sh-122484 cells and different concentrations of LY2109761 on THBS1 -OE cells TGF-β/Smad signalling downstream target genes and THBS1 protein level expression differences. ( f ) Grey scale analysis of the effects of different concentrations of SRI-011381 on the expression of target genes and THBS1 protein level downstream of TGF-β/Smad signalling in THBS1 -sh-122484 cells. ( g ) The effects of different concentrations of LY2109761 on the expression of TGF-β/Smad signalling downstream of TGF-β/Smad protein in THBS1 -OE cells target gene and THBS1 protein level expression grey value analysis. * indicates statistically significant difference (* p < 0.05; ** p < 0.01; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Determination of optimal concentration of TGF-β activator SRI-011381 on THBS1 -sh-122484 cells and inhibitor LY2109761 on THBS1 -OE cells, and its effect on the expression of target genes, THBS1 , downstream of TGF-β/Smad signalling. ( a ) Effect of different concentrations of SRI-011381 on the growth status of THBS1 -sh-122484 cells. ( b ) Effects of different concentrations of LY2109761 on the growth status of THBS1 -OE cells. ( c ) Effects of different concentrations of SRI-011381 on the differential expression of target genes downstream of TGF-β/Smad signalling and THBS1 mRNA levels in THBS1 -sh-122484 cells. ( d ) Effects of different concentrations of LY2109761 on the expression of target genes downstream of TGF-β/Smad signalling and THBS1 , and effects of different concentrations of LY2109761 on the expression of target genes downstream of THBS1 -Sh-122484 cells. LY2109761 on THBS1 -OE cells TGF-β/Smad signalling downstream target genes and THBS1 mRNA level expression differences. ( e ) Effect of different concentrations of SRI-011381 on THBS1 -sh-122484 cells and different concentrations of LY2109761 on THBS1 -OE cells TGF-β/Smad signalling downstream target genes and THBS1 protein level expression differences. ( f ) Grey scale analysis of the effects of different concentrations of SRI-011381 on the expression of target genes and THBS1 protein level downstream of TGF-β/Smad signalling in THBS1 -sh-122484 cells. ( g ) The effects of different concentrations of LY2109761 on the expression of TGF-β/Smad signalling downstream of TGF-β/Smad protein in THBS1 -OE cells target gene and THBS1 protein level expression grey value analysis. * indicates statistically significant difference (* p < 0.05; ** p < 0.01; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Concentration Assay, Expressing, Quantitative Proteomics

Effect of SRI-011381 (5 μg/mL) on the expression of PI3K/Akt , target genes downstream of P53 signalling pathway, SCARB2 in knockdown THBS1 cells ( THBS1 -sh-122484), and LY2109761 (10 μg/mL) in overexpressing THBS1 ( THBS1 -OE) cells. ( a ) SRI-011381 (5 μg/mL) intervention in THBS1 -sh-122484 cells showed differential expression of PI3K/Akt , target genes downstream of P53 signalling, and SCARB2 at the mRNA level. ( b ) LY2109761 (10 μg/mL) intervention in THBS1 -OE cells showed differential expression of PI3K/Akt , target genes downstream of P53 signalling, and SCARB2 expression differences at the mRNA level. ( c ) PI3K/Akt , P53 signalling downstream target genes, and SCARB2 expression differences at the protein level after SRI-011381 (5 μg/mL) intervention in THBS1 -sh-122484 cells and LY2109761 (10 μg/mL) intervention in THBS1 -OE cells. ( d ) SRI-011381 (5 μg/mL) intervention in THBS1 -sh-122484 cells after PI3K/Akt , P53 signalling downstream target genes, and SCARB2 expression differences at protein level grey value analysis. ( e ) LY2109761 (10 μg/mL) intervention in THBS1 -OE cells after PI3K/Akt , P53 signalling downstream target genes, and SCARB2 expression difference at protein level grey value analysis. * indicates statistically significant difference (* p < 0.05; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Effect of SRI-011381 (5 μg/mL) on the expression of PI3K/Akt , target genes downstream of P53 signalling pathway, SCARB2 in knockdown THBS1 cells ( THBS1 -sh-122484), and LY2109761 (10 μg/mL) in overexpressing THBS1 ( THBS1 -OE) cells. ( a ) SRI-011381 (5 μg/mL) intervention in THBS1 -sh-122484 cells showed differential expression of PI3K/Akt , target genes downstream of P53 signalling, and SCARB2 at the mRNA level. ( b ) LY2109761 (10 μg/mL) intervention in THBS1 -OE cells showed differential expression of PI3K/Akt , target genes downstream of P53 signalling, and SCARB2 expression differences at the mRNA level. ( c ) PI3K/Akt , P53 signalling downstream target genes, and SCARB2 expression differences at the protein level after SRI-011381 (5 μg/mL) intervention in THBS1 -sh-122484 cells and LY2109761 (10 μg/mL) intervention in THBS1 -OE cells. ( d ) SRI-011381 (5 μg/mL) intervention in THBS1 -sh-122484 cells after PI3K/Akt , P53 signalling downstream target genes, and SCARB2 expression differences at protein level grey value analysis. ( e ) LY2109761 (10 μg/mL) intervention in THBS1 -OE cells after PI3K/Akt , P53 signalling downstream target genes, and SCARB2 expression difference at protein level grey value analysis. * indicates statistically significant difference (* p < 0.05; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Expressing, Knockdown, Quantitative Proteomics

Effects of SRI-011381 on knockdown THBS1 cells ( THBS1 -sh-122484) and LY2109761 on proliferation and migration ability of overexpressing THBS1 cells ( THBS1 -OE). ( a ) Growth curve of SRI-011381 intervention knockdown THBS1 cells ( THBS1 -sh-122484). ( b , c ) SRI-011381 intervention knockdown THBS1 cells ( THBS1 -sh-122484) migration ability assay. ( d ) SRI-011381 intervention knockdown THBS1 cells ( THBS1 -sh-122484) apoptosis ability assay. ( e ) LY2109761 intervention overexpression THBS1 cells (THBS1-OE) growth curve. ( f , g ) LY2109761 intervention overexpression THBS1 cells ( THBS1 -OE) migration ability assay. ( h ) LY2109761 intervention overexpression THBS1 cells (THBS1-OE) apoptosis ability assay. * indicates statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Effects of SRI-011381 on knockdown THBS1 cells ( THBS1 -sh-122484) and LY2109761 on proliferation and migration ability of overexpressing THBS1 cells ( THBS1 -OE). ( a ) Growth curve of SRI-011381 intervention knockdown THBS1 cells ( THBS1 -sh-122484). ( b , c ) SRI-011381 intervention knockdown THBS1 cells ( THBS1 -sh-122484) migration ability assay. ( d ) SRI-011381 intervention knockdown THBS1 cells ( THBS1 -sh-122484) apoptosis ability assay. ( e ) LY2109761 intervention overexpression THBS1 cells (THBS1-OE) growth curve. ( f , g ) LY2109761 intervention overexpression THBS1 cells ( THBS1 -OE) migration ability assay. ( h ) LY2109761 intervention overexpression THBS1 cells (THBS1-OE) apoptosis ability assay. * indicates statistically significant difference (** p < 0.01; *** p < 0.001) and no * indicates no difference.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Knockdown, Migration, Over Expression

Primer sequences.

Journal: International Journal of Molecular Sciences

Article Title: Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling

doi: 10.3390/ijms26010395

Figure Lengend Snippet: Primer sequences.

Article Snippet: To further evaluate the mechanism by which THBS1 regulates MDCK cell proliferation and apoptosis through TGF-β/Smad signalling, we observed the cell growth status with different concentration gradients of the TGF-β/Smad signalling pathway activator SRI-011381 (MCE, Shanghai, China, HY-12075) of THBS1-knockdown cells ( THBS1 -sh122484) and the inhibitor LY2109761 (MCE, Shanghai, China, HY-100347) of THBS1 -overexpressing cells ( THBS1 -OE).

Techniques: Sequencing