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tnc  (MedChemExpress)
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MedChemExpress tnc
Fig. 4 <t>TNC</t> and FLNC potentially promote EndMT and ECM formation in HCMECs. A-B. Double immunofluorescence (IF) staining of HCMECs treated with culture medium (CM) <t>from</t> <t>TMZ-R</t> cells/TMZ-S cells or with vehicle/recombinant TNC and FLNC proteins using α-SMA (green)/VE-cadherin (red) antibodies. DAPI (blue) was used to label the nucleus. Bars, 200 μm. C. IC50 values and inhibitory curves of etoposide (ETO) and TMZ tested in HCMECs. D. Immunoblot (IB) was used to detect TNC, FLNC and TGF-β alterations in HCMECs treated with TMZ-S/R CM or vehicle/ETO (n = 3, *p < 0.05; **p < 0.01). E. IB was used to detect COL1A1, FN1 and TGF-β expression in HCMECs treated with TMZ-S/R CM or vehicle/TNC and FLNC (TF) recombinant protein (n = 3, *p < 0.05). F. ELISAs were used to detect COL1A1, FN1, TNC and FLNC in supernatants derived from the indicated cells (Blank/ETO, S/R treated) (n = 3, *p < 0.05; **p < 0.01; ***p < 0.001)
Tnc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4 TNC and FLNC potentially promote EndMT and ECM formation in HCMECs. A-B. Double immunofluorescence (IF) staining of HCMECs treated with culture medium (CM) from TMZ-R cells/TMZ-S cells or with vehicle/recombinant TNC and FLNC proteins using α-SMA (green)/VE-cadherin (red) antibodies. DAPI (blue) was used to label the nucleus. Bars, 200 μm. C. IC50 values and inhibitory curves of etoposide (ETO) and TMZ tested in HCMECs. D. Immunoblot (IB) was used to detect TNC, FLNC and TGF-β alterations in HCMECs treated with TMZ-S/R CM or vehicle/ETO (n = 3, *p < 0.05; **p < 0.01). E. IB was used to detect COL1A1, FN1 and TGF-β expression in HCMECs treated with TMZ-S/R CM or vehicle/TNC and FLNC (TF) recombinant protein (n = 3, *p < 0.05). F. ELISAs were used to detect COL1A1, FN1, TNC and FLNC in supernatants derived from the indicated cells (Blank/ETO, S/R treated) (n = 3, *p < 0.05; **p < 0.01; ***p < 0.001)

Journal: Journal of translational medicine

Article Title: Spatial transcriptomics and multi-omics reveal relapse and resistance mechanisms of EndMT-derived CAFs mediated by TNC and FLNC in glioblastoma.

doi: 10.1186/s12967-025-06743-5

Figure Lengend Snippet: Fig. 4 TNC and FLNC potentially promote EndMT and ECM formation in HCMECs. A-B. Double immunofluorescence (IF) staining of HCMECs treated with culture medium (CM) from TMZ-R cells/TMZ-S cells or with vehicle/recombinant TNC and FLNC proteins using α-SMA (green)/VE-cadherin (red) antibodies. DAPI (blue) was used to label the nucleus. Bars, 200 μm. C. IC50 values and inhibitory curves of etoposide (ETO) and TMZ tested in HCMECs. D. Immunoblot (IB) was used to detect TNC, FLNC and TGF-β alterations in HCMECs treated with TMZ-S/R CM or vehicle/ETO (n = 3, *p < 0.05; **p < 0.01). E. IB was used to detect COL1A1, FN1 and TGF-β expression in HCMECs treated with TMZ-S/R CM or vehicle/TNC and FLNC (TF) recombinant protein (n = 3, *p < 0.05). F. ELISAs were used to detect COL1A1, FN1, TNC and FLNC in supernatants derived from the indicated cells (Blank/ETO, S/R treated) (n = 3, *p < 0.05; **p < 0.01; ***p < 0.001)

Article Snippet: HCMECs were seeded in 24-well plates coated with coverslips and treated with TMZ-S/R-CM or 5 μg/ml TNC (MCE) or FLNC (Proteintech) recombinant protein for 48 h. The cells were subsequently fixed with 4% paraformaldehyde (PFA) and processed following our established protocol [19].

Techniques: Immunofluorescence, Staining, Recombinant, Western Blot, Expressing, Derivative Assay

Fig. 6 Schematic overview of Punicalin in treating GBM via blockade ECM deposition and targeting EndMT. CAFs and astrocytes are aboundant in recurrent and chemoresistant GBM patients. FLNC and TNC induced EndMT was a potential source of CAFs in microenvironment of drug-resistant GBM. Punicalin can increase the chemosensitivity of TMZ-resistant tumours by targeted inhibition of TNC and FLNC-induced EndMT and ECM deposition

Journal: Journal of translational medicine

Article Title: Spatial transcriptomics and multi-omics reveal relapse and resistance mechanisms of EndMT-derived CAFs mediated by TNC and FLNC in glioblastoma.

doi: 10.1186/s12967-025-06743-5

Figure Lengend Snippet: Fig. 6 Schematic overview of Punicalin in treating GBM via blockade ECM deposition and targeting EndMT. CAFs and astrocytes are aboundant in recurrent and chemoresistant GBM patients. FLNC and TNC induced EndMT was a potential source of CAFs in microenvironment of drug-resistant GBM. Punicalin can increase the chemosensitivity of TMZ-resistant tumours by targeted inhibition of TNC and FLNC-induced EndMT and ECM deposition

Article Snippet: HCMECs were seeded in 24-well plates coated with coverslips and treated with TMZ-S/R-CM or 5 μg/ml TNC (MCE) or FLNC (Proteintech) recombinant protein for 48 h. The cells were subsequently fixed with 4% paraformaldehyde (PFA) and processed following our established protocol [19].

Techniques: Inhibition