Journal: Heliyon

Article Title: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling pathway

doi: 10.1016/j.heliyon.2023.e22011

Figure Lengend Snippet: Abbreviations: CK-MB, creatine kinase MB isoenzyme; Doxorubicin, Dox; Hematoxylin-eosin, HE; LDH, lactate dehydrogenase; LVEF, left ventricular ejection fraction; LVFS, leave ventricular fractional shortening; NC, Normal Control; TAX, taxifolin. TAX attenuated Dox-induced cardiotoxicity in vivo. (A) Chemical structure of Taxifolin (B) Schematic protocol for mice treatments (C) Kaplan-Meier survival curves of mice (D) Representative images of each group's echocardiogram. (E–F) LVEF and LVFS, n = 6. (G–I) Body weight, heart weight and heart/Tibia index, n = 6 (J–L) LDH, CK- MB, and cTnI levels in mouse serum, n = 6. (M) Representative images of HE staining, original magnification, ×40, scale bar, 500 μm; original magnification, ×400, scale bar, 50 μm. n = 6. Data are means ± SD, ** P < 0.01.

Article Snippet: Taxifolin (C 15 H 12 O 7 , purity ≥99 %, A) was bought from MCE.

Techniques: Control, In Vivo, Staining

Journal: Heliyon

Article Title: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling pathway

doi: 10.1016/j.heliyon.2023.e22011

Figure Lengend Snippet: TAX attenuated Dox-induced ferroptosis in vivo. Abbreviations: DHE, Dihydroethidium; Doxorubicin, Dox; GPX4, Glutathione peroxidase 4; GSH, reduced glutathione; MDA, malondialdehyde; NC, Normal Control; PTGS2, Prostaglandin-endoperoxide synthase 2; SOD, superoxide dismutase; TAX, taxifolin. (A–D) Fe 2+ , MDA, GSH and SOD in vivo, n = 6 (E) Oxidative stress indicator staining, red indicates DHE, original magnification, ×400, scale bar, 50 μm, n = 3. (F) PTGS2 and GPX4 protein expression as assayed by Western blotting in vivo. Unprocessed images of Western blotting were in the supplementary material 1. (G–H) Quantitative analysis of the expression of PTGS2 and GPX4 proteins. The expression levels were normalized to β-actin, n = 3. Data are means ± SD, ** P < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Taxifolin (C 15 H 12 O 7 , purity ≥99 %, A) was bought from MCE.

Techniques: In Vivo, Control, Staining, Expressing, Western Blot

Journal: Heliyon

Article Title: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling pathway

doi: 10.1016/j.heliyon.2023.e22011

Figure Lengend Snippet: TAX attenuated Dox-induced ferroptosis in vitro. Abbreviations: Ctrl, Control; DHE, Dihydroethidium; Doxorubicin, Dox; GPX4, Glutathione peroxidase 4; GSH, reduced glutathione; LDH, lactate dehydrogenase; MDA, malondialdehyde; PTGS2, Prostaglandin-endoperoxide synthase 2; ROS, Reactive Oxygen Species; SOD, superoxide dismutase; TAX, taxifolin. (A) Cell viability assay in each group, n = 6. (B) LDH, n = 6. (C–F) Fe 2+ , MDA, GSH and SOD in H9c2 cells, n = 6 (G) Oxidative stress indicator staining, green indicates ROS and red indicates DHE, original magnification, ×100, scale bar, 200 μm, n = 3. (H) PTGS2 and GPX4 protein expression as assayed by Western blotting in vitro. Unprocessed images of Western blotting were in the supplementary material 1. (I–J) Quantitative analysis of the expression of PTGS2 and GPX4 proteins. The expression levels were normalized to β-actin, n = 3. Data are means ± SD, ** P < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Taxifolin (C 15 H 12 O 7 , purity ≥99 %, A) was bought from MCE.

Techniques: In Vitro, Control, Viability Assay, Staining, Expressing, Western Blot

Journal: Heliyon

Article Title: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling pathway

doi: 10.1016/j.heliyon.2023.e22011

Figure Lengend Snippet: TAX activated Nrf2 signal pathway in vivo and in vitro. Abbreviations: Ctrl, Control; Doxorubicin, Dox; GPX4, Glutathione peroxidase 4; HO-1, Heme Oxygenase-1; NQO1, NADPH: Quinone Oxidoreductase 1; Nrf2, Nuclear factor erythroid 2-related factor 2; TAX, taxifolin. (A) Nrf2, HO-1 and NQO1 protein expression as assayed by Western blotting in vivo. Unprocessed images of Western blotting were in the supplementary material 1. (B–D) Quantitative analysis of the expression of Nrf2, HO-1 and NQO1 proteins. The expression levels were normalized to β-actin, n = 3. (E) Nrf2, HO-1 and NQO1 protein expression as assayed by Western blotting in vitro. Unprocessed images of Western blotting were in the supplementary material 1. (F–H) Quantitative analysis of the expression of Nrf2, HO-1 and NQO1 proteins. The expression levels were normalized to β-actin, n = 3. (I) Immunofluorescence staining of Nrf2 in each group, original magnification, ×400, scale bar, 50 μm, n = 3. Data are means ± SD, * P < 0.05; ** P < 0.01.

Article Snippet: Taxifolin (C 15 H 12 O 7 , purity ≥99 %, A) was bought from MCE.

Techniques: In Vivo, In Vitro, Control, Expressing, Western Blot, Immunofluorescence, Staining

Journal: Heliyon

Article Title: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling pathway

doi: 10.1016/j.heliyon.2023.e22011

Figure Lengend Snippet: TAX attenuated doxorubicin-induced ferroptosis via Nrf2 signal pathway in vitro. Abbreviations: Doxorubicin, Dox; GPX4, Glutathione peroxidase 4; GSH, reduced glutathione; HO-1, Heme Oxygenase-1; LDH, lactate dehydrogenase; MDA, malondialdehyde; NC, Normal Control; NQO1, NADPH: Quinone Oxidoreductase 1; Nrf2, Nuclear factor erythroid 2-related factor 2; PTGS2, Prostaglandin-endoperoxide synthase 2; SOD, superoxide dismutase; TAX, taxifolin. (A) Nrf2, HO-1 and NQO1 protein expression as assayed by Western blotting in vitro. Unprocessed images of Western blotting were in the supplementary material 1. (B–D) Quantitative analysis of the expression of Nrf2, HO-1 and NQO1 proteins. The expression levels were normalized to β-actin, n = 3. (E) LDH, n = 6. (F–I) Fe 2+ , MDA, GSH and SOD in vitro, n = 6 (J) PTGS2 and GPX4 protein expression as assayed by Western blotting in vitro. Unprocessed images of Western blotting were in the supplementary material 1. (K–L) Quantitative analysis of the expression of PTGS2 and GPX4 proteins. The expression levels were normalized to β-actin, n = 3. Data are means ± SD, * P < 0.05; ** P < 0.01.

Article Snippet: Taxifolin (C 15 H 12 O 7 , purity ≥99 %, A) was bought from MCE.

Techniques: In Vitro, Control, Expressing, Western Blot

Journal: Heliyon

Article Title: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling pathway

doi: 10.1016/j.heliyon.2023.e22011

Figure Lengend Snippet: TAX adjusted Nrf2 signal pathway and attenuated ferroptosis by miR-200a. Abbreviations: Doxorubicin, Dox; GPX4, Glutathione peroxidase 4; GSH, reduced glutathione; HO-1, Heme Oxygenase-1; Keap1, Kelch-like ECH-associated protein 1; LDH, lactate dehydrogenase; MDA, malondialdehyde; NC, Normal Control; NQO1, NADPH: Quinone Oxidoreductase 1; Nrf2, Nuclear factor erythroid 2-related factor 2; PTGS2, Prostaglandin-endoperoxide synthase 2; SOD, superoxide dismutase; TAX, taxifolin. (A) The expression levels of Nrf2-related miRNA in H9c2 cells. (B) Nrf2, HO-1 and NQO1 protein expression as assayed by Western blotting in vitro. Unprocessed images of Western blotting were in the supplementary material 1. (C) The expression levels of Keap1 mRNA, n = 6. (D) Cell viability assay in each group, n = 6. (E) LDH, n = 6. (F–H) Quantitative analysis of the expression of Nrf2, HO-1 and NQO1 proteins. The expression levels were normalized to β-actin, n = 3. (I–L) Fe 2+ , MDA, GSH and SOD in vitro, n = 6 (M) PTGS2 and GPX4 protein expression as assayed by Western blotting in vitro. Unprocessed images of Western blotting were in the supplementary material 1. (N–O) Quantitative analysis of the expression of PTGS2 and GPX4 proteins. The expression levels were normalized to β-actin, n = 3. Data are means ± SD, * P < 0.05; ** P < 0.01.

Article Snippet: Taxifolin (C 15 H 12 O 7 , purity ≥99 %, A) was bought from MCE.

Techniques: Control, Expressing, Western Blot, In Vitro, Viability Assay

Journal: Heliyon

Article Title: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling pathway

doi: 10.1016/j.heliyon.2023.e22011

Figure Lengend Snippet: Schematic: Taxifolin protects against doxorubicin-induced cardiotoxicity and ferroptosis by adjusting microRNA-200a-mediated Nrf2 signaling.

Article Snippet: Taxifolin (C 15 H 12 O 7 , purity ≥99 %, A) was bought from MCE.

Techniques: