Journal: Pharmaceuticals

Article Title: Anti-COVID Drugs (MMV COVID Box) as Leishmanicidal Agents: Unveiling New Therapeutic Horizons

doi: 10.3390/ph17030266

Figure Lengend Snippet: Leishmanicidal activity against the promastigote stage of the selected compounds from MMV COVID Box. Values correspond to inhibitory concentrations 50 ± standard deviation (μM). w/a: without activity.

Article Snippet: After an initial screening, five pure compounds were purchased: Terconazole was obtained from Cymit Química S.L. (Pamplona, Spain); ABT-239 was purchased from MedChemExpress (Monmouth Junction, NJ, USA); and Bortezomib, Almitrine mesylate, and Midostaurin hydrate were purchased from Sigma-Aldrich (now Merck) (Darmstadt, Germany).

Techniques: Activity Assay, Standard Deviation

Journal: Pharmaceuticals

Article Title: Anti-COVID Drugs (MMV COVID Box) as Leishmanicidal Agents: Unveiling New Therapeutic Horizons

doi: 10.3390/ph17030266

Figure Lengend Snippet: 2D Chemical structures of the selected active compounds.

Article Snippet: After an initial screening, five pure compounds were purchased: Terconazole was obtained from Cymit Química S.L. (Pamplona, Spain); ABT-239 was purchased from MedChemExpress (Monmouth Junction, NJ, USA); and Bortezomib, Almitrine mesylate, and Midostaurin hydrate were purchased from Sigma-Aldrich (now Merck) (Darmstadt, Germany).

Techniques:

Journal: Pharmaceuticals

Article Title: Anti-COVID Drugs (MMV COVID Box) as Leishmanicidal Agents: Unveiling New Therapeutic Horizons

doi: 10.3390/ph17030266

Figure Lengend Snippet: Leishmanicidal activity against the amastigote stage of the selected compounds from MMV COVID Box and cytotoxic activity against murine macrophages (μM). Selectivity index (CC 50 /IC 50 ). w/a: without activity; n/d: not determined.

Article Snippet: After an initial screening, five pure compounds were purchased: Terconazole was obtained from Cymit Química S.L. (Pamplona, Spain); ABT-239 was purchased from MedChemExpress (Monmouth Junction, NJ, USA); and Bortezomib, Almitrine mesylate, and Midostaurin hydrate were purchased from Sigma-Aldrich (now Merck) (Darmstadt, Germany).

Techniques: Activity Assay

Journal: Pharmaceuticals

Article Title: Anti-COVID Drugs (MMV COVID Box) as Leishmanicidal Agents: Unveiling New Therapeutic Horizons

doi: 10.3390/ph17030266

Figure Lengend Snippet: Transmitted light, SytoxGreen (green), Hoechst (blue), and Propidium iodide (red) staining. Results after 24 h of incubation of L. amazonensis promastigotes with the IC90 of compounds. Negative control ( A – D ). Bortezomib ( E – H ); almitrine ( I – L ); terconazole ( M – P ); and ABT239 ( Q – T ). Images were captured using an EVOS FL Cell Imaging system (Thermo Fisher Scientific) (40×). Scale: 75 µm.

Article Snippet: After an initial screening, five pure compounds were purchased: Terconazole was obtained from Cymit Química S.L. (Pamplona, Spain); ABT-239 was purchased from MedChemExpress (Monmouth Junction, NJ, USA); and Bortezomib, Almitrine mesylate, and Midostaurin hydrate were purchased from Sigma-Aldrich (now Merck) (Darmstadt, Germany).

Techniques: Staining, Incubation, Negative Control, Imaging

Journal: Pharmaceuticals

Article Title: Anti-COVID Drugs (MMV COVID Box) as Leishmanicidal Agents: Unveiling New Therapeutic Horizons

doi: 10.3390/ph17030266

Figure Lengend Snippet: CellROX Deep Red staining. Results after 24 h of incubation of L. amazonensis promastigotes with the IC 90 of the compounds. Negative control ( A , B ); Positive control treated with H 2 O 2 ( E , F ); Bortezomib ( C , D ); almitrine ( G , H ); terconazole ( I , J ); and ABT-239 ( K , L ). Images were captured using an EVOS FL Cell Imaging system (Thermo Fisher Scientific) (100×). Scale: 20µm.

Article Snippet: After an initial screening, five pure compounds were purchased: Terconazole was obtained from Cymit Química S.L. (Pamplona, Spain); ABT-239 was purchased from MedChemExpress (Monmouth Junction, NJ, USA); and Bortezomib, Almitrine mesylate, and Midostaurin hydrate were purchased from Sigma-Aldrich (now Merck) (Darmstadt, Germany).

Techniques: Staining, Incubation, Negative Control, Positive Control, Imaging