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MedChemExpress citicoline
( A ) Schematic diagram displaying the time course for <t>citicoline</t> treatment, imaging, and TEM analysis. TEM, transmission electron microscope. ( B ) Bright field imaging shows the normal morphology and the area (7 - 12 somites) being analyzed in . ( C ) Measurements of larval length and eye area (n ≥ 14 fish). ( D ) The fluorescent images of Tg(MBPa:eGFP-CAAX) transgenic line by control with vehicle, control with citicoline, Slc44a1b -/- with vehicle, and Slc44a1b -/- with citicoline. ( E ) Quantification of MBP + area and intensity in the dorsal and ventral SC (n ≥ 14 fish). ( F ) Schematic of the transverse section of a 7 dpf larval zebrafish at the level of the urogenital opening. Inset, transverse section of the SC at the same level. Myelinated (yellow) axons are located in the dorsal and ventral spinal tracts of the SC (CNS). m, Mauthner axons. ( G ) TEM images of the myelinated tracts in the dorsal (top rows) and ventral SC (bottom rows). Yellow arrows indicate myelinated axon. Red arrows indicate unmyelinated axon. ( H ) Quantification of dorsal and ventral myelinated axons per area (n = 5 fish).
Citicoline, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Schematic diagram displaying the time course for citicoline treatment, imaging, and TEM analysis. TEM, transmission electron microscope. ( B ) Bright field imaging shows the normal morphology and the area (7 - 12 somites) being analyzed in . ( C ) Measurements of larval length and eye area (n ≥ 14 fish). ( D ) The fluorescent images of Tg(MBPa:eGFP-CAAX) transgenic line by control with vehicle, control with citicoline, Slc44a1b -/- with vehicle, and Slc44a1b -/- with citicoline. ( E ) Quantification of MBP + area and intensity in the dorsal and ventral SC (n ≥ 14 fish). ( F ) Schematic of the transverse section of a 7 dpf larval zebrafish at the level of the urogenital opening. Inset, transverse section of the SC at the same level. Myelinated (yellow) axons are located in the dorsal and ventral spinal tracts of the SC (CNS). m, Mauthner axons. ( G ) TEM images of the myelinated tracts in the dorsal (top rows) and ventral SC (bottom rows). Yellow arrows indicate myelinated axon. Red arrows indicate unmyelinated axon. ( H ) Quantification of dorsal and ventral myelinated axons per area (n = 5 fish).

Journal: bioRxiv

Article Title: SLC44A1 deficiency impedes myelin development in the central nervous system

doi: 10.1101/2025.04.08.647819

Figure Lengend Snippet: ( A ) Schematic diagram displaying the time course for citicoline treatment, imaging, and TEM analysis. TEM, transmission electron microscope. ( B ) Bright field imaging shows the normal morphology and the area (7 - 12 somites) being analyzed in . ( C ) Measurements of larval length and eye area (n ≥ 14 fish). ( D ) The fluorescent images of Tg(MBPa:eGFP-CAAX) transgenic line by control with vehicle, control with citicoline, Slc44a1b -/- with vehicle, and Slc44a1b -/- with citicoline. ( E ) Quantification of MBP + area and intensity in the dorsal and ventral SC (n ≥ 14 fish). ( F ) Schematic of the transverse section of a 7 dpf larval zebrafish at the level of the urogenital opening. Inset, transverse section of the SC at the same level. Myelinated (yellow) axons are located in the dorsal and ventral spinal tracts of the SC (CNS). m, Mauthner axons. ( G ) TEM images of the myelinated tracts in the dorsal (top rows) and ventral SC (bottom rows). Yellow arrows indicate myelinated axon. Red arrows indicate unmyelinated axon. ( H ) Quantification of dorsal and ventral myelinated axons per area (n = 5 fish).

Article Snippet: The experimental and control groups were administered 100 mg/kg citicoline (MedChemExpress, Catalog #33818-15-4) as previously described or an equal volume of physiological saline via intraperitoneal injection, respectively.

Techniques: Imaging, Transmission Assay, Microscopy, Transgenic Assay, Control

( A ) Schematic diagram displaying the time course for citicoline treatment. TEM, transmission electron microscope. ( B ) Black gold myelin staining and quantification of relative myelin fibers density. ( C ) TEM images and quantification of percentage of myelinated axons in the ON and CC.

Journal: bioRxiv

Article Title: SLC44A1 deficiency impedes myelin development in the central nervous system

doi: 10.1101/2025.04.08.647819

Figure Lengend Snippet: ( A ) Schematic diagram displaying the time course for citicoline treatment. TEM, transmission electron microscope. ( B ) Black gold myelin staining and quantification of relative myelin fibers density. ( C ) TEM images and quantification of percentage of myelinated axons in the ON and CC.

Article Snippet: The experimental and control groups were administered 100 mg/kg citicoline (MedChemExpress, Catalog #33818-15-4) as previously described or an equal volume of physiological saline via intraperitoneal injection, respectively.

Techniques: Transmission Assay, Microscopy, Staining