HY-90009S Search Results


tadalafil  (MedChemExpress)
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MedChemExpress tadalafil
Chemical synthesis of chemicals A, B, and C. ( A ) Molecular docking scores of <t>tadalafil</t> and some existing PRMT5 inhibitors with PRMT5. ( B ) Chemical structures and molecular docking scores of chemicals A, B, C, and tadalafil.
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Chemical synthesis of chemicals A, B, and C. ( A ) Molecular docking scores of tadalafil and some existing PRMT5 inhibitors with PRMT5. ( B ) Chemical structures and molecular docking scores of chemicals A, B, C, and tadalafil.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemical synthesis of chemicals A, B, and C. ( A ) Molecular docking scores of tadalafil and some existing PRMT5 inhibitors with PRMT5. ( B ) Chemical structures and molecular docking scores of chemicals A, B, C, and tadalafil.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques:

Chemicals A, B, and C bind to PRMT5 and inhibit histone arginine methylation. ( A ) Binding abilities of chemicals A, B, and C to the PRMT5/MEP50 complex detected by Biacore T200. ( B ) MDA-MB-231 cells were treated with the indicated concentrations of chemicals A, B, C, and tadalafil for 48 h, and the global protein levels of H4R3me2s and H3R8me2s were detected by immunoblotting. The picture is representative of two independent experiments.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C bind to PRMT5 and inhibit histone arginine methylation. ( A ) Binding abilities of chemicals A, B, and C to the PRMT5/MEP50 complex detected by Biacore T200. ( B ) MDA-MB-231 cells were treated with the indicated concentrations of chemicals A, B, C, and tadalafil for 48 h, and the global protein levels of H4R3me2s and H3R8me2s were detected by immunoblotting. The picture is representative of two independent experiments.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques: Methylation, Binding Assay, Western Blot

Chemicals A, B, and C inhibit the viability of breast cancer cells. ( A – C ) MDA-MB-231 cells ( A ), MCF-7 cells ( B ), and HCC1937 cells ( C ) were treated with the indicated concentrations of chemicals A, B, C, and tadalafil for 48 h, and cell viability was determined by the CCK-8 assay. The cell viability at 150 μM was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C inhibit the viability of breast cancer cells. ( A – C ) MDA-MB-231 cells ( A ), MCF-7 cells ( B ), and HCC1937 cells ( C ) were treated with the indicated concentrations of chemicals A, B, C, and tadalafil for 48 h, and cell viability was determined by the CCK-8 assay. The cell viability at 150 μM was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques: CCK-8 Assay

Chemicals A, B, and C suppress the DNA replication of breast cancer cells. MDA-MB-231 cells were treated with chemicals A, B, C, and tadalafil at 150 μM for 48 h, and cell proliferation was detected by EdU assays. ( A ) Representative images of MDA-MB-231 cells treated with chemicals A, B, C, and tadalafil. Scale bar, 100 μm. ( B ) The percentage of EdU-positive (EdU + ) cells was quantitatively compared ( n = 8). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C suppress the DNA replication of breast cancer cells. MDA-MB-231 cells were treated with chemicals A, B, C, and tadalafil at 150 μM for 48 h, and cell proliferation was detected by EdU assays. ( A ) Representative images of MDA-MB-231 cells treated with chemicals A, B, C, and tadalafil. Scale bar, 100 μm. ( B ) The percentage of EdU-positive (EdU + ) cells was quantitatively compared ( n = 8). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques:

Chemicals A, B, and C promote the apoptosis of MDA-MB-231 cells. MDA-MB-231 cells were treated with chemicals A, B, C, and tadalafil at 150 μM for 48 h, and apoptosis was detected by annexin V/PI staining and FACS. ( A ) Representative plots for the flow cytometric analysis of cell apoptosis. ( B ) Quantification of the cell percentage at different stages of apoptosis ( n = 3). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C promote the apoptosis of MDA-MB-231 cells. MDA-MB-231 cells were treated with chemicals A, B, C, and tadalafil at 150 μM for 48 h, and apoptosis was detected by annexin V/PI staining and FACS. ( A ) Representative plots for the flow cytometric analysis of cell apoptosis. ( B ) Quantification of the cell percentage at different stages of apoptosis ( n = 3). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques: Staining

Chemicals A, B, and C promote the apoptosis of MCF-7 cells. MCF-7 cells were treated with chemicals A, B, C, and tadalafil at 150 μM for 48 h, and apoptosis was detected by annexin V/PI staining and FACS. ( A ) Representative plots for the flow cytometric analysis of cell apoptosis. ( B ) Quantification of the cell percentage at different stages of apoptosis ( n = 3). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C promote the apoptosis of MCF-7 cells. MCF-7 cells were treated with chemicals A, B, C, and tadalafil at 150 μM for 48 h, and apoptosis was detected by annexin V/PI staining and FACS. ( A ) Representative plots for the flow cytometric analysis of cell apoptosis. ( B ) Quantification of the cell percentage at different stages of apoptosis ( n = 3). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques: Staining

Chemicals A, B, and C enhance the inhibitory effects of doxorubicin on cell viability. ( A , B ) MDA-MB-231 ( A ) and MCF-7 cells ( B ) were treated with chemicals A, B, C, and tadalafil at 150 μM in combination with the indicated concentrations of doxorubicin for 48 h. The cell viability at 0.2 μg/mL doxorubicin treatment was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C enhance the inhibitory effects of doxorubicin on cell viability. ( A , B ) MDA-MB-231 ( A ) and MCF-7 cells ( B ) were treated with chemicals A, B, C, and tadalafil at 150 μM in combination with the indicated concentrations of doxorubicin for 48 h. The cell viability at 0.2 μg/mL doxorubicin treatment was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques:

Chemicals A, B, and C enhance the inhibitory effects of cisplatin on cell viability. ( A , B ) MDA-MB-231 ( A ) and MCF-7 cells ( B ) were treated with chemicals A, B, C, and tadalafil at 150 μM in combination with the indicated concentrations of cisplatin for 48 h. The cell viability at 16 μM cisplatin treatment was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C enhance the inhibitory effects of cisplatin on cell viability. ( A , B ) MDA-MB-231 ( A ) and MCF-7 cells ( B ) were treated with chemicals A, B, C, and tadalafil at 150 μM in combination with the indicated concentrations of cisplatin for 48 h. The cell viability at 16 μM cisplatin treatment was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques:

Chemicals A, B, and C enhance the inhibitory effects of olaparib on cell viability. ( A , B ) MDA-MB-231 ( A ) and MCF-7 cells ( B ) were treated with chemicals A, B, C, and tadalafil at 150 μM in combination with the indicated concentrations of olaparib for 48 h. The cell viability at 10 μM olaparib treatment was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemicals A, B, and C enhance the inhibitory effects of olaparib on cell viability. ( A , B ) MDA-MB-231 ( A ) and MCF-7 cells ( B ) were treated with chemicals A, B, C, and tadalafil at 150 μM in combination with the indicated concentrations of olaparib for 48 h. The cell viability at 10 μM olaparib treatment was quantitatively compared ( n = 6). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques:

Chemical A improves the pro-apoptotic effects of doxorubicin. MDA-MB-231 cells were treated with 150 μM chemical A and tadalafil in combination with 0.2 μg/mL doxorubicin for 48 h, and apoptosis was detected with annexin V/PI staining and FACS ( n = 3). ( A ) Representative plots for the flow cytometric analysis of cell apoptosis. ( B ) Quantification of the cell percentage at different stages of apoptosis ( n = 3). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Journal: International Journal of Molecular Sciences

Article Title: Novel Chemicals Derived from Tadalafil Exhibit PRMT5 Inhibition and Promising Activities against Breast Cancer

doi: 10.3390/ijms23094806

Figure Lengend Snippet: Chemical A improves the pro-apoptotic effects of doxorubicin. MDA-MB-231 cells were treated with 150 μM chemical A and tadalafil in combination with 0.2 μg/mL doxorubicin for 48 h, and apoptosis was detected with annexin V/PI staining and FACS ( n = 3). ( A ) Representative plots for the flow cytometric analysis of cell apoptosis. ( B ) Quantification of the cell percentage at different stages of apoptosis ( n = 3). The line and error bars indicate the mean ± s.e.m.; one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Treatments with cisplatin (Selleck, S1166, Houston, TX, USA), doxorubicin (Selleck, S1208), olaparib (Selleck, S1060), and tadalafil (MCE, HY-90009A, Monmouth Junction, NJ, USA) were performed as described in the main text.

Techniques: Staining