HY-17386B Search Results


rosiglitazone  (MedChemExpress)
99
MedChemExpress rosiglitazone
The PPARγ-HK2 pathway promotes the efferocytosis of LPMs against ferroptotic THP-1 cells. ( A ) The mRNA expression level of PPARγ in LPMs incubated with nontreated or Erastin-treated THP-1 cells for 4 h (normalised to β-actin). ( B ) Cell viability of LPMs treated with 10 µM PPARγ agonist, <t>Rosiglitazone</t> or 10 µM PPARγ inhibitor, T0070907 for 18 h. ( C , D ) LPMs treated as above were incubated with nontreated or Erastin-treated THP-1 cells for 4 h. Efferocytosis of LPMs from each group was determined by ( C ) flow cytometry and ( D ) confocal microscope (scale bar: 50 μm). ( E ) The mRNA expression level of HK2 in LPMs treated as above was detected by qRT-PCR. ( F , G ) qRT-PCR analysis ( F ) and Western blotting ( G ) showed the mRNA and protein levels of HK2 in LPMs transfected with three interfering fragments of HK2 (si-HK2) for 24 h. Full-length blots are presented in Supplementary Fig. 6. ( H , I ) LPMs were transfected with siHK2 for 24 h before stimulated with 10 µM Rosiglitazone, then incubated with Erastin-treated THP-1 cells stained by CFSE for 4 h. Efferocytosis of LPMs from each group was determined by ( H ) flow cytometry and ( I ) confocal microscope (scale bar: 40 μm). Values are mean ± SD. * p < 0.05, ** p < 0.01, ns denotes p > 0.05 (by unpaired Student’s t test).
Rosiglitazone, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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agonist rosiglitazone  (MedChemExpress)
94
MedChemExpress agonist rosiglitazone
The PPARγ-HK2 pathway promotes the efferocytosis of LPMs against ferroptotic THP-1 cells. ( A ) The mRNA expression level of PPARγ in LPMs incubated with nontreated or Erastin-treated THP-1 cells for 4 h (normalised to β-actin). ( B ) Cell viability of LPMs treated with 10 µM PPARγ agonist, <t>Rosiglitazone</t> or 10 µM PPARγ inhibitor, T0070907 for 18 h. ( C , D ) LPMs treated as above were incubated with nontreated or Erastin-treated THP-1 cells for 4 h. Efferocytosis of LPMs from each group was determined by ( C ) flow cytometry and ( D ) confocal microscope (scale bar: 50 μm). ( E ) The mRNA expression level of HK2 in LPMs treated as above was detected by qRT-PCR. ( F , G ) qRT-PCR analysis ( F ) and Western blotting ( G ) showed the mRNA and protein levels of HK2 in LPMs transfected with three interfering fragments of HK2 (si-HK2) for 24 h. Full-length blots are presented in Supplementary Fig. 6. ( H , I ) LPMs were transfected with siHK2 for 24 h before stimulated with 10 µM Rosiglitazone, then incubated with Erastin-treated THP-1 cells stained by CFSE for 4 h. Efferocytosis of LPMs from each group was determined by ( H ) flow cytometry and ( I ) confocal microscope (scale bar: 40 μm). Values are mean ± SD. * p < 0.05, ** p < 0.01, ns denotes p > 0.05 (by unpaired Student’s t test).
Agonist Rosiglitazone, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The PPARγ-HK2 pathway promotes the efferocytosis of LPMs against ferroptotic THP-1 cells. ( A ) The mRNA expression level of PPARγ in LPMs incubated with nontreated or Erastin-treated THP-1 cells for 4 h (normalised to β-actin). ( B ) Cell viability of LPMs treated with 10 µM PPARγ agonist, Rosiglitazone or 10 µM PPARγ inhibitor, T0070907 for 18 h. ( C , D ) LPMs treated as above were incubated with nontreated or Erastin-treated THP-1 cells for 4 h. Efferocytosis of LPMs from each group was determined by ( C ) flow cytometry and ( D ) confocal microscope (scale bar: 50 μm). ( E ) The mRNA expression level of HK2 in LPMs treated as above was detected by qRT-PCR. ( F , G ) qRT-PCR analysis ( F ) and Western blotting ( G ) showed the mRNA and protein levels of HK2 in LPMs transfected with three interfering fragments of HK2 (si-HK2) for 24 h. Full-length blots are presented in Supplementary Fig. 6. ( H , I ) LPMs were transfected with siHK2 for 24 h before stimulated with 10 µM Rosiglitazone, then incubated with Erastin-treated THP-1 cells stained by CFSE for 4 h. Efferocytosis of LPMs from each group was determined by ( H ) flow cytometry and ( I ) confocal microscope (scale bar: 40 μm). Values are mean ± SD. * p < 0.05, ** p < 0.01, ns denotes p > 0.05 (by unpaired Student’s t test).

Journal: Stem Cell Research & Therapy

Article Title: MSCs promote the efferocytosis of large peritoneal macrophages to eliminate ferroptotic monocytes/macrophages in the injured endometria

doi: 10.1186/s13287-024-03742-z

Figure Lengend Snippet: The PPARγ-HK2 pathway promotes the efferocytosis of LPMs against ferroptotic THP-1 cells. ( A ) The mRNA expression level of PPARγ in LPMs incubated with nontreated or Erastin-treated THP-1 cells for 4 h (normalised to β-actin). ( B ) Cell viability of LPMs treated with 10 µM PPARγ agonist, Rosiglitazone or 10 µM PPARγ inhibitor, T0070907 for 18 h. ( C , D ) LPMs treated as above were incubated with nontreated or Erastin-treated THP-1 cells for 4 h. Efferocytosis of LPMs from each group was determined by ( C ) flow cytometry and ( D ) confocal microscope (scale bar: 50 μm). ( E ) The mRNA expression level of HK2 in LPMs treated as above was detected by qRT-PCR. ( F , G ) qRT-PCR analysis ( F ) and Western blotting ( G ) showed the mRNA and protein levels of HK2 in LPMs transfected with three interfering fragments of HK2 (si-HK2) for 24 h. Full-length blots are presented in Supplementary Fig. 6. ( H , I ) LPMs were transfected with siHK2 for 24 h before stimulated with 10 µM Rosiglitazone, then incubated with Erastin-treated THP-1 cells stained by CFSE for 4 h. Efferocytosis of LPMs from each group was determined by ( H ) flow cytometry and ( I ) confocal microscope (scale bar: 40 μm). Values are mean ± SD. * p < 0.05, ** p < 0.01, ns denotes p > 0.05 (by unpaired Student’s t test).

Article Snippet: Erastin (Catalog # HY-15,763), Cytochalasin D (Catalog # HY-N6682), 2-Deoxy-D-glucose (Catalog # HY-13,966), Rosiglitazone (Catalog # HY-17,386), T0070907 (Catalog # HY-13,202), and Bafilomycin A1 (Catalog # HY-100,558) were purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: Expressing, Incubation, Flow Cytometry, Microscopy, Quantitative RT-PCR, Western Blot, Transfection, Staining