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gw9662  (MedChemExpress)
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MedChemExpress gw9662
A. Screen of the down-regulated molecules Stat5 from the proteomics analysis performed on the jejunum of JWA wt and JWA ko mice. B. Immunoblotting of Stat5a, Stat5b and JWA in the jejunum of JWA wt and JWA ko mice; n=3 for each genotype. C. Immunoblotting of PPARγ, Stat5a, Stat5b, JAK2 and p-JAK2 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. D. QRT–PCR detection of Stat5a, Stat5b and PPARγ levels in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. E. QRT–PCR detection of Notch target genes hes1, hes2, hes5, hes6 and hes7 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. F. Immunoblotting of full-length Notch1, cleaved Notch1, NICD and hes1 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. G. QRT–PCR detection of Notch1 levels in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. H. Immunoblotting of JWA, Stat5a, PPARγ, hes1, NICD and Notch1 in IEC-6 cells transfected with shJWA plasmids. I. Immunoblotting of JWA, Stat5a, PPARγ and hes1 in IEC-6 cells co-transfected with shJWA and HA-hes1 plasmids. J. Immunoblotting of JWA, Stat5a and PPARγ in IEC-6 cells transfected with shJWA plasmid followed by <t>GW9662</t> (10 μM) treatment. Data information: In (D, E, G), data are presented as mean ± SD, * P <0.05 and *** P <0.001 (Student’s t -test).
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A. Screen of the down-regulated molecules Stat5 from the proteomics analysis performed on the jejunum of JWA wt and JWA ko mice. B. Immunoblotting of Stat5a, Stat5b and JWA in the jejunum of JWA wt and JWA ko mice; n=3 for each genotype. C. Immunoblotting of PPARγ, Stat5a, Stat5b, JAK2 and p-JAK2 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. D. QRT–PCR detection of Stat5a, Stat5b and PPARγ levels in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. E. QRT–PCR detection of Notch target genes hes1, hes2, hes5, hes6 and hes7 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. F. Immunoblotting of full-length Notch1, cleaved Notch1, NICD and hes1 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. G. QRT–PCR detection of Notch1 levels in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. H. Immunoblotting of JWA, Stat5a, PPARγ, hes1, NICD and Notch1 in IEC-6 cells transfected with shJWA plasmids. I. Immunoblotting of JWA, Stat5a, PPARγ and hes1 in IEC-6 cells co-transfected with shJWA and HA-hes1 plasmids. J. Immunoblotting of JWA, Stat5a and PPARγ in IEC-6 cells transfected with shJWA plasmid followed by GW9662 (10 μM) treatment. Data information: In (D, E, G), data are presented as mean ± SD, * P <0.05 and *** P <0.001 (Student’s t -test).

Journal: bioRxiv

Article Title: JWA deficiency accelerates aging through disrupting intestinal epithelial homeostasis via Notch1/PPARγ/Stat5 axis

doi: 10.1101/2022.01.17.476552

Figure Lengend Snippet: A. Screen of the down-regulated molecules Stat5 from the proteomics analysis performed on the jejunum of JWA wt and JWA ko mice. B. Immunoblotting of Stat5a, Stat5b and JWA in the jejunum of JWA wt and JWA ko mice; n=3 for each genotype. C. Immunoblotting of PPARγ, Stat5a, Stat5b, JAK2 and p-JAK2 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. D. QRT–PCR detection of Stat5a, Stat5b and PPARγ levels in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. E. QRT–PCR detection of Notch target genes hes1, hes2, hes5, hes6 and hes7 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. F. Immunoblotting of full-length Notch1, cleaved Notch1, NICD and hes1 in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. G. QRT–PCR detection of Notch1 levels in the crypts of JWA IEC-wt and JWA IEC-ko mice; n=3 for each genotype. H. Immunoblotting of JWA, Stat5a, PPARγ, hes1, NICD and Notch1 in IEC-6 cells transfected with shJWA plasmids. I. Immunoblotting of JWA, Stat5a, PPARγ and hes1 in IEC-6 cells co-transfected with shJWA and HA-hes1 plasmids. J. Immunoblotting of JWA, Stat5a and PPARγ in IEC-6 cells transfected with shJWA plasmid followed by GW9662 (10 μM) treatment. Data information: In (D, E, G), data are presented as mean ± SD, * P <0.05 and *** P <0.001 (Student’s t -test).

Article Snippet: To activate the phosphorylation of ERK1/2 or suppress the expression of PPARγ, the cell was treated with 10 μM pamoic acid (Macklin, Shanghai, China) or 10 μM GW9662 (MedChemExpress, Monmouth Junction, NJ, USA) for 24 hours.

Techniques: Western Blot, Quantitative RT-PCR, Transfection, Plasmid Preparation