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pacritinib  (MedChemExpress)
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MedChemExpress pacritinib
(A) Cytokines known to support the growth of B cells were measured in conditioned media from cells infected only with EBV, from non-immortalized and immortalized co-transformed cells, and from established PEL cell lines (KSHV + EBV + or KSHV + EBV - ). (B) Co-transformed cells were grown at a density of 5X10 4 cells/ml in basal medium or in 50% conditioned medium. (*: p < 0.05) (C) Co-transformed cells were grown in complete medium or complete medium supplemented with rapamycin (100 nM) or <t>pacritinib</t> (100 nM) for 48 hours. (**: p < 0.01; ***: p < 0.001).
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(A) Cytokines known to support the growth of B cells were measured in conditioned media from cells infected only with EBV, from non-immortalized and immortalized co-transformed cells, and from established PEL cell lines (KSHV + EBV + or KSHV + EBV - ). (B) Co-transformed cells were grown at a density of 5X10 4 cells/ml in basal medium or in 50% conditioned medium. (*: p < 0.05) (C) Co-transformed cells were grown in complete medium or complete medium supplemented with rapamycin (100 nM) or pacritinib (100 nM) for 48 hours. (**: p < 0.01; ***: p < 0.001).

Journal: PLOS Pathogens

Article Title: Kaposi sarcoma-associated herpesvirus cooperates with Epstein-Barr virus to co-transform a small set of human B cells oncogenically

doi: 10.1371/journal.ppat.1013281

Figure Lengend Snippet: (A) Cytokines known to support the growth of B cells were measured in conditioned media from cells infected only with EBV, from non-immortalized and immortalized co-transformed cells, and from established PEL cell lines (KSHV + EBV + or KSHV + EBV - ). (B) Co-transformed cells were grown at a density of 5X10 4 cells/ml in basal medium or in 50% conditioned medium. (*: p < 0.05) (C) Co-transformed cells were grown in complete medium or complete medium supplemented with rapamycin (100 nM) or pacritinib (100 nM) for 48 hours. (**: p < 0.01; ***: p < 0.001).

Article Snippet: Cells were grown in complete medium (RPMI-1640 supplemented with FBS and antibiotics as described previously) or complete medium supplemented with 100nM Rapamycin (Tocris Bioscience, Minneapolis MN) or 100nM Pacritinib (MedChem Express, Monmouth Junction NJ) for 48 hours.

Techniques: Infection, Transformation Assay