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fh535  (MedChemExpress)
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MedChemExpress fh535
TF activity profile of cell culture (A) Round bar plot showing the TF activity profile of HEK293T, MCF7, and MCF10A cells. TF activity as calculated by Rep expression divided by Ref expression value are shown. ∗p < 0.05, Dunnett’s test compared with MCF7, n = 3–7. (B and C) Pharmacological effect on TF activity. HEK293T cells transfected with TCF/LEF (green) and CREB (red) reporter viruses were treated with 200 μM forskolin (B) or 20 mM LiCl (C) for 3, 6, or 9 h. Log 2 fold change of TF activities compared with 0 h are shown. ∗p < 0.01, Dunnett’s test compared with 0 h, n = 12. (D and E) Effect of inhibitors. Cells were pretreated with 10 μM NASTRp or 25 μM <t>FH535</t> and stimulated with forskolin or LiCl for 9 h. Log 2 fold change of TF activities compared with the control-treated culture (Cnt) are shown. ∗p < 0.01, Sidak’s test, n = 12. Mean ± SEM are shown.
Fh535, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TF activity profile of cell culture (A) Round bar plot showing the TF activity profile of HEK293T, MCF7, and MCF10A cells. TF activity as calculated by Rep expression divided by Ref expression value are shown. ∗p < 0.05, Dunnett’s test compared with MCF7, n = 3–7. (B and C) Pharmacological effect on TF activity. HEK293T cells transfected with TCF/LEF (green) and CREB (red) reporter viruses were treated with 200 μM forskolin (B) or 20 mM LiCl (C) for 3, 6, or 9 h. Log 2 fold change of TF activities compared with 0 h are shown. ∗p < 0.01, Dunnett’s test compared with 0 h, n = 12. (D and E) Effect of inhibitors. Cells were pretreated with 10 μM NASTRp or 25 μM FH535 and stimulated with forskolin or LiCl for 9 h. Log 2 fold change of TF activities compared with the control-treated culture (Cnt) are shown. ∗p < 0.01, Sidak’s test, n = 12. Mean ± SEM are shown.

Journal: iScience

Article Title: PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery

doi: 10.1016/j.isci.2022.103927

Figure Lengend Snippet: TF activity profile of cell culture (A) Round bar plot showing the TF activity profile of HEK293T, MCF7, and MCF10A cells. TF activity as calculated by Rep expression divided by Ref expression value are shown. ∗p < 0.05, Dunnett’s test compared with MCF7, n = 3–7. (B and C) Pharmacological effect on TF activity. HEK293T cells transfected with TCF/LEF (green) and CREB (red) reporter viruses were treated with 200 μM forskolin (B) or 20 mM LiCl (C) for 3, 6, or 9 h. Log 2 fold change of TF activities compared with 0 h are shown. ∗p < 0.01, Dunnett’s test compared with 0 h, n = 12. (D and E) Effect of inhibitors. Cells were pretreated with 10 μM NASTRp or 25 μM FH535 and stimulated with forskolin or LiCl for 9 h. Log 2 fold change of TF activities compared with the control-treated culture (Cnt) are shown. ∗p < 0.01, Sidak’s test, n = 12. Mean ± SEM are shown.

Article Snippet: In the drug treatment experiments, HEK293T cells transfected with lentiviruses were treated with Naphthol AS-TR phosphate (NASTPp; 10 μM, TCI), FH535 (20 μM, MedChemExpress), forskolin (200 μM, WAKO), or LiCl (20 mM, WAKO).

Techniques: Activity Assay, Cell Culture, Expressing, Transfection, Control

Journal: iScience

Article Title: PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery

doi: 10.1016/j.isci.2022.103927

Figure Lengend Snippet:

Article Snippet: In the drug treatment experiments, HEK293T cells transfected with lentiviruses were treated with Naphthol AS-TR phosphate (NASTPp; 10 μM, TCI), FH535 (20 μM, MedChemExpress), forskolin (200 μM, WAKO), or LiCl (20 mM, WAKO).

Techniques: Virus, Recombinant, Software