HY-157107 Search Results


vehicle dmso  (MedChemExpress)
92
MedChemExpress vehicle dmso
Co-inhibition of GR and β-catenin re-sensitizes DTX-resistant PCa cells to docetaxel. ( A ) Hoffman modulation microscopy (800×) images of DTX-sensitive and DTX-resistant 22rv1, PC3, and DU145 cells treated <t>with</t> <t>CORT-108297</t> (CORT, 1 μM), MSAB (1 μM), or CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) for 72 h in adherent cultures. ( B ) Cell viability following treatments was assessed using MTT assays. DMSO was used for vehicle control. ( C ) Apoptotic index (fold induction of apoptosis) was determined by flow cytometry using Annexin V and 7AAD for PC3-DR and DU145-DR cells treated with the GR and β-catenin inhibitors . DTX-resistant cells, but not sensitive cells, were cultured throughout the treatment in medium containing 10 nM DTX. All treatments were done in cells cultured in media supplemented with CS-FBS and 10 nM dexamethasone. Data are representative from 3 independent experiments and represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. The scale bar for all images is set at 20 μm.
Vehicle Dmso, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vehicle dmso/product/MedChemExpress
Average 92 stars, based on 1 article reviews
vehicle dmso - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier

Image Search Results


Co-inhibition of GR and β-catenin re-sensitizes DTX-resistant PCa cells to docetaxel. ( A ) Hoffman modulation microscopy (800×) images of DTX-sensitive and DTX-resistant 22rv1, PC3, and DU145 cells treated with CORT-108297 (CORT, 1 μM), MSAB (1 μM), or CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) for 72 h in adherent cultures. ( B ) Cell viability following treatments was assessed using MTT assays. DMSO was used for vehicle control. ( C ) Apoptotic index (fold induction of apoptosis) was determined by flow cytometry using Annexin V and 7AAD for PC3-DR and DU145-DR cells treated with the GR and β-catenin inhibitors . DTX-resistant cells, but not sensitive cells, were cultured throughout the treatment in medium containing 10 nM DTX. All treatments were done in cells cultured in media supplemented with CS-FBS and 10 nM dexamethasone. Data are representative from 3 independent experiments and represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. The scale bar for all images is set at 20 μm.

Journal: International Journal of Molecular Sciences

Article Title: Glucocorticoid Receptor and β-Catenin Interact in Prostate Cancer Cells and Their Co-Inhibition Attenuates Tumorsphere Formation, Stemness, and Docetaxel Resistance

doi: 10.3390/ijms24087130

Figure Lengend Snippet: Co-inhibition of GR and β-catenin re-sensitizes DTX-resistant PCa cells to docetaxel. ( A ) Hoffman modulation microscopy (800×) images of DTX-sensitive and DTX-resistant 22rv1, PC3, and DU145 cells treated with CORT-108297 (CORT, 1 μM), MSAB (1 μM), or CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) for 72 h in adherent cultures. ( B ) Cell viability following treatments was assessed using MTT assays. DMSO was used for vehicle control. ( C ) Apoptotic index (fold induction of apoptosis) was determined by flow cytometry using Annexin V and 7AAD for PC3-DR and DU145-DR cells treated with the GR and β-catenin inhibitors . DTX-resistant cells, but not sensitive cells, were cultured throughout the treatment in medium containing 10 nM DTX. All treatments were done in cells cultured in media supplemented with CS-FBS and 10 nM dexamethasone. Data are representative from 3 independent experiments and represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. The scale bar for all images is set at 20 μm.

Article Snippet: Briefly, adherent DTX-resistant PCa cells were harvested at 75% confluency and seeded at 10,000 cells/mL in Tumorsphere XF medium containing either vehicle (DMSO), CORT-108297 (1 μM), MSAB (1 μM), CORT-108297 plus MSAB (1 μM each), or the BET bromodomain inhibitor JQ1 (1 μM) (MedChemExpress, Monmouth Junction, NJ, USA, Cat# HY-13030), and plated onto 60 mm ultra-low attachment dishes (Corning, Glendale, AZ, USA, Cat# 3261).

Techniques: Inhibition, Microscopy, Control, Flow Cytometry, Cell Culture

Co-inhibition of GR and β-catenin impairs docetaxel-resistant prostate tumorsphere formation. DTX-resistant 22rv1-DR ( A ), PC3-DR ( C ), and DU145-DR ( E ) cells were cultured as tumorspheres in ultra-low adherence dishes in Tumorsphere XF medium containing 10 nM DTX and vehicle (DMSO), CORT-108297 (CORT, 1 μM), MSAB (1 μM), or CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) for 6 days and imaged using an Olympus IX70 microscope equipped with Hoffman modulation. Images were acquired at 4× or 40× magnification. The scale bar for all images (4× magnification) is set at 100 μm. Tumorsphere area was quantified from quadruplicate images for each cell line ( B , D , F ). Data are representative from 3 independent experiments and are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Glucocorticoid Receptor and β-Catenin Interact in Prostate Cancer Cells and Their Co-Inhibition Attenuates Tumorsphere Formation, Stemness, and Docetaxel Resistance

doi: 10.3390/ijms24087130

Figure Lengend Snippet: Co-inhibition of GR and β-catenin impairs docetaxel-resistant prostate tumorsphere formation. DTX-resistant 22rv1-DR ( A ), PC3-DR ( C ), and DU145-DR ( E ) cells were cultured as tumorspheres in ultra-low adherence dishes in Tumorsphere XF medium containing 10 nM DTX and vehicle (DMSO), CORT-108297 (CORT, 1 μM), MSAB (1 μM), or CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) for 6 days and imaged using an Olympus IX70 microscope equipped with Hoffman modulation. Images were acquired at 4× or 40× magnification. The scale bar for all images (4× magnification) is set at 100 μm. Tumorsphere area was quantified from quadruplicate images for each cell line ( B , D , F ). Data are representative from 3 independent experiments and are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Briefly, adherent DTX-resistant PCa cells were harvested at 75% confluency and seeded at 10,000 cells/mL in Tumorsphere XF medium containing either vehicle (DMSO), CORT-108297 (1 μM), MSAB (1 μM), CORT-108297 plus MSAB (1 μM each), or the BET bromodomain inhibitor JQ1 (1 μM) (MedChemExpress, Monmouth Junction, NJ, USA, Cat# HY-13030), and plated onto 60 mm ultra-low attachment dishes (Corning, Glendale, AZ, USA, Cat# 3261).

Techniques: Inhibition, Cell Culture, Microscopy

Co-inhibition of GR and β-catenin reduces the percent of cells with stemness markers in docetaxel-resistant tumorspheres. ( A ) Post-acquisition gating strategy, shown in a representative PC3-DR analysis. Data are depicted as pseudo color plots that denote population densities (high: red/orange, intermediate: yellow, low: blue/green) in bivariate settings (X-Y plane). The color densities do not refer to the spectral emission of the cells depicted, but rather the density of cells relative to one another. Doublet discrimination and fluorescence-minus-one controls (graph insets) are depicted with smoothing to better visualize the gating strategy. ( B ) CD44+ expression presented as percent of live (7AAD-negative) cells staining positive for CD44 in 22rv1-DR, PC3-DR, and DU145-DR cells from tumorspheres treated with CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) compared to vehicle (DMSO) control. ( C ) CD44+ CD24– expression presented as percent of live cells staining positive for CD44 and negative for CD24 in 22rv1-DR, PC3-DR, and DU145-DR cells from tumorspheres treated with CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) compared with vehicle control. Data are representative from 3 independent experiments and are represented as mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: Glucocorticoid Receptor and β-Catenin Interact in Prostate Cancer Cells and Their Co-Inhibition Attenuates Tumorsphere Formation, Stemness, and Docetaxel Resistance

doi: 10.3390/ijms24087130

Figure Lengend Snippet: Co-inhibition of GR and β-catenin reduces the percent of cells with stemness markers in docetaxel-resistant tumorspheres. ( A ) Post-acquisition gating strategy, shown in a representative PC3-DR analysis. Data are depicted as pseudo color plots that denote population densities (high: red/orange, intermediate: yellow, low: blue/green) in bivariate settings (X-Y plane). The color densities do not refer to the spectral emission of the cells depicted, but rather the density of cells relative to one another. Doublet discrimination and fluorescence-minus-one controls (graph insets) are depicted with smoothing to better visualize the gating strategy. ( B ) CD44+ expression presented as percent of live (7AAD-negative) cells staining positive for CD44 in 22rv1-DR, PC3-DR, and DU145-DR cells from tumorspheres treated with CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) compared to vehicle (DMSO) control. ( C ) CD44+ CD24– expression presented as percent of live cells staining positive for CD44 and negative for CD24 in 22rv1-DR, PC3-DR, and DU145-DR cells from tumorspheres treated with CORT-108297 plus MSAB (CORT + MSAB, 1 μM each) compared with vehicle control. Data are representative from 3 independent experiments and are represented as mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Briefly, adherent DTX-resistant PCa cells were harvested at 75% confluency and seeded at 10,000 cells/mL in Tumorsphere XF medium containing either vehicle (DMSO), CORT-108297 (1 μM), MSAB (1 μM), CORT-108297 plus MSAB (1 μM each), or the BET bromodomain inhibitor JQ1 (1 μM) (MedChemExpress, Monmouth Junction, NJ, USA, Cat# HY-13030), and plated onto 60 mm ultra-low attachment dishes (Corning, Glendale, AZ, USA, Cat# 3261).

Techniques: Inhibition, Fluorescence, Expressing, Staining, Control