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d phe  (MedChemExpress)
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MedChemExpress d phe
Synthesis and characterization of DSRGT. a Schematic representation of the synthesis and molecular interaction mechanism of DSRGT. b Solution-to-gel transition. c 1 H nuclear magnetic resonance (NMR) spectra of TD-198946 and TD-198946-PEG-AC in D 2 O. d 1 H NMR spectra of Glu and Glu-PEG-AC in D 2 O. e Fourier transform infrared spectroscopy (FTIR) spectra of DSR, DSRG, DSRT, and DSRGT. f Macroscopic images of spheres printed with DSR, DSRG, DSRT, and DSRGT in the DLP system. Scale bar = 500 µm. g Field-emission scanning electron microscopy (FE-SEM) images of spheres printed with DSR, DSRG, DSRT, and DSRGT in the DLP system. Scale bar = 500 µm. h FE-SEM images of bulk hydrogels: DSR, DSRG, DSRT, and DSRGT. i Porosity ( n = 6) and pore sizes ( n = 150) of DSR, DSRG, DSRT, and DSRGT. Scale bar = 400 µm (upper) and 200 µm (lower). j Rheological curves of DSR, DSRG, DSRT, and DSRGT. k Viscosity curves of DSR, DSRG, DSRT, and DSRGT. l Degradation rates of DSR, DSRG, DSRT, and DSRGT ( n = 6). m Swelling ratios of DSR, DSRG, DSRT, and DSRGT ( n = 6). n Release profiles of Glu and TD-198946 from DSRGT. Data are presented as mean ± SD. One-way ANOVA and Tukey’s multiple-comparisons test were used for data analysis. ns non-significant, SilMA silk fibroin methacrylate, Glu glucosamine, AC-PEG-NHS acrylic acid-polyethylene glycol-N-hydroxysuccinimide, DNA-SF DNA-silk fibroin, Pep-RGDfKAC RGD-containing peptide <t>modified</t> <t>with</t> <t>D-Phe-Lys</t> and an AC functional group, UV ultraviolet, DSR RGD-modified DNA-silk fibroin hydrogel, DSRG Glu-containing DSR, DSRT TD-198946-containing DSR, DSRGT DNA-silk fibroin hydrogel sustained-release system, DLP digital light processing, G' storage modulus, G'' loss modulus, η* complex viscosity
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Synthesis and characterization of DSRGT. a Schematic representation of the synthesis and molecular interaction mechanism of DSRGT. b Solution-to-gel transition. c 1 H nuclear magnetic resonance (NMR) spectra of TD-198946 and TD-198946-PEG-AC in D 2 O. d 1 H NMR spectra of Glu and Glu-PEG-AC in D 2 O. e Fourier transform infrared spectroscopy (FTIR) spectra of DSR, DSRG, DSRT, and DSRGT. f Macroscopic images of spheres printed with DSR, DSRG, DSRT, and DSRGT in the DLP system. Scale bar = 500 µm. g Field-emission scanning electron microscopy (FE-SEM) images of spheres printed with DSR, DSRG, DSRT, and DSRGT in the DLP system. Scale bar = 500 µm. h FE-SEM images of bulk hydrogels: DSR, DSRG, DSRT, and DSRGT. i Porosity ( n = 6) and pore sizes ( n = 150) of DSR, DSRG, DSRT, and DSRGT. Scale bar = 400 µm (upper) and 200 µm (lower). j Rheological curves of DSR, DSRG, DSRT, and DSRGT. k Viscosity curves of DSR, DSRG, DSRT, and DSRGT. l Degradation rates of DSR, DSRG, DSRT, and DSRGT ( n = 6). m Swelling ratios of DSR, DSRG, DSRT, and DSRGT ( n = 6). n Release profiles of Glu and TD-198946 from DSRGT. Data are presented as mean ± SD. One-way ANOVA and Tukey’s multiple-comparisons test were used for data analysis. ns non-significant, SilMA silk fibroin methacrylate, Glu glucosamine, AC-PEG-NHS acrylic acid-polyethylene glycol-N-hydroxysuccinimide, DNA-SF DNA-silk fibroin, Pep-RGDfKAC RGD-containing peptide modified with D-Phe-Lys and an AC functional group, UV ultraviolet, DSR RGD-modified DNA-silk fibroin hydrogel, DSRG Glu-containing DSR, DSRT TD-198946-containing DSR, DSRGT DNA-silk fibroin hydrogel sustained-release system, DLP digital light processing, G' storage modulus, G'' loss modulus, η* complex viscosity

Journal: Military Medical Research

Article Title: Accelerating cartilage regeneration with DNA-SF hydrogel sustained release system-based cartilage organoids

doi: 10.1186/s40779-025-00625-z

Figure Lengend Snippet: Synthesis and characterization of DSRGT. a Schematic representation of the synthesis and molecular interaction mechanism of DSRGT. b Solution-to-gel transition. c 1 H nuclear magnetic resonance (NMR) spectra of TD-198946 and TD-198946-PEG-AC in D 2 O. d 1 H NMR spectra of Glu and Glu-PEG-AC in D 2 O. e Fourier transform infrared spectroscopy (FTIR) spectra of DSR, DSRG, DSRT, and DSRGT. f Macroscopic images of spheres printed with DSR, DSRG, DSRT, and DSRGT in the DLP system. Scale bar = 500 µm. g Field-emission scanning electron microscopy (FE-SEM) images of spheres printed with DSR, DSRG, DSRT, and DSRGT in the DLP system. Scale bar = 500 µm. h FE-SEM images of bulk hydrogels: DSR, DSRG, DSRT, and DSRGT. i Porosity ( n = 6) and pore sizes ( n = 150) of DSR, DSRG, DSRT, and DSRGT. Scale bar = 400 µm (upper) and 200 µm (lower). j Rheological curves of DSR, DSRG, DSRT, and DSRGT. k Viscosity curves of DSR, DSRG, DSRT, and DSRGT. l Degradation rates of DSR, DSRG, DSRT, and DSRGT ( n = 6). m Swelling ratios of DSR, DSRG, DSRT, and DSRGT ( n = 6). n Release profiles of Glu and TD-198946 from DSRGT. Data are presented as mean ± SD. One-way ANOVA and Tukey’s multiple-comparisons test were used for data analysis. ns non-significant, SilMA silk fibroin methacrylate, Glu glucosamine, AC-PEG-NHS acrylic acid-polyethylene glycol-N-hydroxysuccinimide, DNA-SF DNA-silk fibroin, Pep-RGDfKAC RGD-containing peptide modified with D-Phe-Lys and an AC functional group, UV ultraviolet, DSR RGD-modified DNA-silk fibroin hydrogel, DSRG Glu-containing DSR, DSRT TD-198946-containing DSR, DSRGT DNA-silk fibroin hydrogel sustained-release system, DLP digital light processing, G' storage modulus, G'' loss modulus, η* complex viscosity

Article Snippet: To prepare the DSRGT solution, the following components were dissolved and mixed in PBS (pH 7.4) at 37 °C: 10 wt% SilMA (EFL, China), 0.25 wt% lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), 5 wt% RGD-containing peptide modified with D-Phe-Lys and an AC functional group (Pep-RGDfKAC), 100 nmol/L TD-198946 (MCE, USA), 10 mmol/L Glu (MCE, USA), 4.5 wt% AC-PEG-NHS (EFL, China), and 500,000 nmol/L ssDNA (Sangon Biotech, China).

Techniques: Nuclear Magnetic Resonance, Fourier Transform Infrared Spectroscopy, Spectroscopy, Electron Microscopy, Viscosity, Modification, Functional Assay

DNA-SF sustained-release system synthesis and its mechanism for cartilage repair. Glu glucosamine, BMSCs bone-marrow mesenchymal stem cells, AC-PEG-NHS acrylic acid-polyethylene glycol-N-hydroxysuccinimide, ECM extracellular matrix, SilMA silk fibroin methacrylate, UV ultraviolet, DNA-SF DNA-silk fibroin, Pep-RGDfKAC RGD-containing peptide modified with D-Phe-Lys and an AC functional group, MAPK mitogen-activated protein kinase

Journal: Military Medical Research

Article Title: Accelerating cartilage regeneration with DNA-SF hydrogel sustained release system-based cartilage organoids

doi: 10.1186/s40779-025-00625-z

Figure Lengend Snippet: DNA-SF sustained-release system synthesis and its mechanism for cartilage repair. Glu glucosamine, BMSCs bone-marrow mesenchymal stem cells, AC-PEG-NHS acrylic acid-polyethylene glycol-N-hydroxysuccinimide, ECM extracellular matrix, SilMA silk fibroin methacrylate, UV ultraviolet, DNA-SF DNA-silk fibroin, Pep-RGDfKAC RGD-containing peptide modified with D-Phe-Lys and an AC functional group, MAPK mitogen-activated protein kinase

Article Snippet: To prepare the DSRGT solution, the following components were dissolved and mixed in PBS (pH 7.4) at 37 °C: 10 wt% SilMA (EFL, China), 0.25 wt% lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), 5 wt% RGD-containing peptide modified with D-Phe-Lys and an AC functional group (Pep-RGDfKAC), 100 nmol/L TD-198946 (MCE, USA), 10 mmol/L Glu (MCE, USA), 4.5 wt% AC-PEG-NHS (EFL, China), and 500,000 nmol/L ssDNA (Sangon Biotech, China).

Techniques: Modification, Functional Assay