HY-15434A Search Results


ng25  (MedChemExpress)
94
MedChemExpress ng25
TAK1 inhibition restores eNOS levels and ameliorates ethanol-induced liver injury, oxidative stress, and inflammation caused by Txnip deficiency in LSECs. (A-C) LSECs isolated from Txnip -/- and WT mice were incubated with <t>NG25</t> (500 nM) for 3 h, incubated with LPS (0.75 μg/ml) for 0.5 or 6 h, and subjected to western blot analysis (A), qRT-PCR analysis (B and C), and NO assay (B) (n = 4). (D-G) Txnip fl/fl and Txnip ΔEC mice were fed a 5% ethanol diet with NG25 (5 mg/kg/day) for 4 weeks (4w EtOH) and subjected to H&E staining (D), blood chemistry analysis (E), oxidative stress analysis (F), and qRT-PCR analysis (G) (n = 5). Scale bars, 50 μM. Data are presented as means ± SD. * p < 0.05; ** p < 0.01. “ns” stands for “not significant.”
Ng25, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ng25/product/MedChemExpress
Average 94 stars, based on 1 article reviews
ng25 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier

Image Search Results


TAK1 inhibition restores eNOS levels and ameliorates ethanol-induced liver injury, oxidative stress, and inflammation caused by Txnip deficiency in LSECs. (A-C) LSECs isolated from Txnip -/- and WT mice were incubated with NG25 (500 nM) for 3 h, incubated with LPS (0.75 μg/ml) for 0.5 or 6 h, and subjected to western blot analysis (A), qRT-PCR analysis (B and C), and NO assay (B) (n = 4). (D-G) Txnip fl/fl and Txnip ΔEC mice were fed a 5% ethanol diet with NG25 (5 mg/kg/day) for 4 weeks (4w EtOH) and subjected to H&E staining (D), blood chemistry analysis (E), oxidative stress analysis (F), and qRT-PCR analysis (G) (n = 5). Scale bars, 50 μM. Data are presented as means ± SD. * p < 0.05; ** p < 0.01. “ns” stands for “not significant.”

Journal: International Journal of Biological Sciences

Article Title: TXNIP in liver sinusoidal endothelial cells ameliorates alcohol-associated liver disease via nitric oxide production

doi: 10.7150/ijbs.90781

Figure Lengend Snippet: TAK1 inhibition restores eNOS levels and ameliorates ethanol-induced liver injury, oxidative stress, and inflammation caused by Txnip deficiency in LSECs. (A-C) LSECs isolated from Txnip -/- and WT mice were incubated with NG25 (500 nM) for 3 h, incubated with LPS (0.75 μg/ml) for 0.5 or 6 h, and subjected to western blot analysis (A), qRT-PCR analysis (B and C), and NO assay (B) (n = 4). (D-G) Txnip fl/fl and Txnip ΔEC mice were fed a 5% ethanol diet with NG25 (5 mg/kg/day) for 4 weeks (4w EtOH) and subjected to H&E staining (D), blood chemistry analysis (E), oxidative stress analysis (F), and qRT-PCR analysis (G) (n = 5). Scale bars, 50 μM. Data are presented as means ± SD. * p < 0.05; ** p < 0.01. “ns” stands for “not significant.”

Article Snippet: For NG25 treatment, Txnip ΔEC and Txnip fl/fl mice were fed a liquid diet containing 5% ethanol for 4 weeks, with further administration of NG25 (5 mg/kg/day intraperitoneally; MedChemExpress, Monmouth Junction, NJ, USA) or vehicle.

Techniques: Inhibition, Isolation, Incubation, Western Blot, Quantitative RT-PCR, Staining