Journal: Parasites & Vectors

Article Title: A novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4–ROS–NLRP3 pathway

doi: 10.1186/s13071-022-05466-6

Figure Lengend Snippet: Primers of the detected genes

Article Snippet: Approximately 100 µl MDM suspension (1 × 10 6 /ml) was treated respectively with PBS, pET-32a, rBsCPI-1, BsES and Nigericin sodium salt, a NLRP3 activator (10 μM; MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Sequencing

Journal: Parasites & Vectors

Article Title: A novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4–ROS–NLRP3 pathway

doi: 10.1186/s13071-022-05466-6

Figure Lengend Snippet: rBsCPI-1 and BsES induced inflammasome activation. A Quantitative PCR analysis of the transcription levels of the NOD-like receptor 3 (NLRP3) inflammasome-related genes in MDMs. B , C A representative western blot band of ASC and cleaved N-terminal gasdermin D (GSDMD) are shown in B , and a graph of the quantified band density is shown in C . D The enzyme-linked immunosorbent assay (ELISA) detected the secreted IL-1β and IL-18 in the cell supernatant. E , F A representative western blotting band of retained IL-1β and IL-18 in the cells are shown in E , and the graph of the quantified band density is shown in F . Data are shown as mean ± SD of 3 replicates per group. Significant differences versus the control group are indicated by * P < 0.05, ** P < 0.01, *** P < 0.001; versus pET-32a group by § P < 0.05, §§ P < 0.01, §§§ P < 0.001; versus the rBsCPI-1 group by & P < 0.05, && P < 0.01, &&& P < 0.001; and versus the BsES group by # P < 0.05, ## P < 0.01, ### P < 0.001. GSDMD, Gasdermin D; IL, interleukin

Article Snippet: Approximately 100 µl MDM suspension (1 × 10 6 /ml) was treated respectively with PBS, pET-32a, rBsCPI-1, BsES and Nigericin sodium salt, a NLRP3 activator (10 μM; MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Activation Assay, Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Parasites & Vectors

Article Title: A novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4–ROS–NLRP3 pathway

doi: 10.1186/s13071-022-05466-6

Figure Lengend Snippet: rBsCPI-1 affects NLRP3 activation through the TLR4-ROS-NLRP3 pathway. A The fluorescence intensity of intracellular fluorogenic dye 2′-7′dichlorofluorescin diacetate (DCFH-DA) was detected at 488 nm excitation and 525 nm emission wavelengths. The data presented are representative of 3 independent experiments. Significant differences versus the control group (PBS + PBS) are indicated by * P < 0.05, *** P < 0.001; for the LPS + CPI-1 group versus the LPS + PBS group by &&& P < 0.001; for the 7E3 + CPI-1 group versus the 7E3 + PBS group by # P < 0.05; and for the LPS + CPI-1 group versus the LPS + PBS group by && P < 0.01. B , C A representative western blot is shown in B , and the graph of the quantified band density is shown in C . C * P < 0.05; versus the NAC + PBS group by § P < 0.05; versus PBS + CPI-1 group by &&& P < 0.001. D Fluorescence intensity of intracellular DCFH-DA. Significant differences versus the control group are indicated by * P < 0.05, ** P < 0.01, *** P < 0.001; for the MCC 950 + CPI-1 group or Nigericin + CPI-1 group versus the PBS + CPI-1 group by § P < 0.05; for the Nigericin + CPI-1 group versus the Nigericin + PBS group by & P < 0.05. 7E3, TLR-4-blocking antibody; LPS, lipopolysaccharide (TLR4 activator); MCC 950, MCC950 Sodium (a NLRP3 inhibitor); NAC, N -acetylcysteine (a ROS inhibitor); ROS, reactive oxygen species; TLR4, Toll-like receptor 4

Article Snippet: Approximately 100 µl MDM suspension (1 × 10 6 /ml) was treated respectively with PBS, pET-32a, rBsCPI-1, BsES and Nigericin sodium salt, a NLRP3 activator (10 μM; MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Activation Assay, Fluorescence, Control, Western Blot, Blocking Assay

Journal: Parasites & Vectors

Article Title: A novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4–ROS–NLRP3 pathway

doi: 10.1186/s13071-022-05466-6

Figure Lengend Snippet: The activation of NLRP3 inflammasome-induced pyroptosis. a b A representative western blotting is shown in A , and the graph of the quantified band density is shown in B . Data are shown as the mean ± SD of 3 replicates per group. Significant differences versus the control group are indicated by * P < 0.05, ** P < 0.01, *** P < 0.001; versus the MCC 950 + PBS group by § P < 0.05; versus the PBS + CPI-1 group by & P < 0.05, &&& P < 0.001

Article Snippet: Approximately 100 µl MDM suspension (1 × 10 6 /ml) was treated respectively with PBS, pET-32a, rBsCPI-1, BsES and Nigericin sodium salt, a NLRP3 activator (10 μM; MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Activation Assay, Western Blot, Control

Journal: Parasites & Vectors

Article Title: A novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4–ROS–NLRP3 pathway

doi: 10.1186/s13071-022-05466-6

Figure Lengend Snippet: Analysis of the activation of the NLRP3 inflammasome in vivo. Data are shown as the mean ± SD of 3 replicates per group. Significant differences versus the control group are indicated by * P < 0.05, ** P < 0.01, *** P < 0.001; significant differences versus the pET-32a group are indicated by § P < 0.05, §§ P < 0.01, §§§ P < 0.001

Article Snippet: Approximately 100 µl MDM suspension (1 × 10 6 /ml) was treated respectively with PBS, pET-32a, rBsCPI-1, BsES and Nigericin sodium salt, a NLRP3 activator (10 μM; MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Activation Assay, In Vivo, Control