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hsp70 inhibitor mkt 077  (MedChemExpress)
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MedChemExpress hsp70 inhibitor mkt 077
Aspirin enhanced the heat shock response in the calcification process of VSMCs. (A) The expression of heat shock proteins in the process of VSMC calcification. Western blot assay was used to detected protein expression of HSF1, <t>HSP70</t> and HSP90 in VSMCs after different-time calcification induction. β-Actin was used as loading control. (B–D) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01. (E) The effect of aspirin on expression of heat shock proteins in VSMCs after four-day culture in OIM. Western blot assay was used to detect protein expression of HSF1, HSP70 and HSP90 in VSMCs. β-Actin was used as loading control. (F–H) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01.
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Aspirin enhanced the heat shock response in the calcification process of VSMCs. (A) The expression of heat shock proteins in the process of VSMC calcification. Western blot assay was used to detected protein expression of HSF1, HSP70 and HSP90 in VSMCs after different-time calcification induction. β-Actin was used as loading control. (B–D) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01. (E) The effect of aspirin on expression of heat shock proteins in VSMCs after four-day culture in OIM. Western blot assay was used to detect protein expression of HSF1, HSP70 and HSP90 in VSMCs. β-Actin was used as loading control. (F–H) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01.

Journal: Pharmaceutical Biology

Article Title: Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response

doi: 10.1080/13880209.2021.2007268

Figure Lengend Snippet: Aspirin enhanced the heat shock response in the calcification process of VSMCs. (A) The expression of heat shock proteins in the process of VSMC calcification. Western blot assay was used to detected protein expression of HSF1, HSP70 and HSP90 in VSMCs after different-time calcification induction. β-Actin was used as loading control. (B–D) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01. (E) The effect of aspirin on expression of heat shock proteins in VSMCs after four-day culture in OIM. Western blot assay was used to detect protein expression of HSF1, HSP70 and HSP90 in VSMCs. β-Actin was used as loading control. (F–H) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01.

Article Snippet: Aspirin (1 mmol/L or 4 mmol/L; Sangon Biotech, Shanghai, China), HSP70 inhibitor MKT-077 (1 µmol/L; MedChemExpress, Monmouth Junction, NJ) and HSP90 inhibitor 17-DMAG (1 µmol/L; Sigma, St. Louis, MO) were applied to treat the calcification-induced VSMCs.

Techniques: Expressing, Western Blot, Control

Inhibition of HSP70 counteracted the anti-calcification effect of aspirin on VSMCs. (A) The effect of HSP70 inhibitor MKT-077 on aspirin alleviating VSMC calcification. Alizarin Red S assay was applied to detect calcium nodules in VSMCs after 10-day culture in OIM. (B) The effect of MKT-077 on aspirin reducing intracellular calcium concentration in VSMCs after 10-day culture in OIM. n = 5 in each group. ** p < 0.01. (C) The effect of MKT-077 on aspirin downregulating osteogenic marker genes expression in VSMCs after four-day culture in OIM. β-Actin was used as loading control. (D, E) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01. (F) The effect of HSP70/HSP90 siRNA on aspirin alleviating VSMC calcification. The calcium nodules were detected by Alizarin Red S assay in VSMCs after 10-day culture in OIM. (G) The effect of HSP70/HSP90 siRNA on aspirin reducing intracellular calcium concentration in VSMCs after 10-day culture in OIM. n = 5 in each group. * p < 0.05.

Journal: Pharmaceutical Biology

Article Title: Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response

doi: 10.1080/13880209.2021.2007268

Figure Lengend Snippet: Inhibition of HSP70 counteracted the anti-calcification effect of aspirin on VSMCs. (A) The effect of HSP70 inhibitor MKT-077 on aspirin alleviating VSMC calcification. Alizarin Red S assay was applied to detect calcium nodules in VSMCs after 10-day culture in OIM. (B) The effect of MKT-077 on aspirin reducing intracellular calcium concentration in VSMCs after 10-day culture in OIM. n = 5 in each group. ** p < 0.01. (C) The effect of MKT-077 on aspirin downregulating osteogenic marker genes expression in VSMCs after four-day culture in OIM. β-Actin was used as loading control. (D, E) Grey values analysis of immune bands in western blot assay. n = 3 in each group. ** p < 0.01. (F) The effect of HSP70/HSP90 siRNA on aspirin alleviating VSMC calcification. The calcium nodules were detected by Alizarin Red S assay in VSMCs after 10-day culture in OIM. (G) The effect of HSP70/HSP90 siRNA on aspirin reducing intracellular calcium concentration in VSMCs after 10-day culture in OIM. n = 5 in each group. * p < 0.05.

Article Snippet: Aspirin (1 mmol/L or 4 mmol/L; Sangon Biotech, Shanghai, China), HSP70 inhibitor MKT-077 (1 µmol/L; MedChemExpress, Monmouth Junction, NJ) and HSP90 inhibitor 17-DMAG (1 µmol/L; Sigma, St. Louis, MO) were applied to treat the calcification-induced VSMCs.

Techniques: Inhibition, Concentration Assay, Marker, Expressing, Control, Western Blot