HY-14848A Search Results


usp7  (MedChemExpress)
93
MedChemExpress usp7
A Western blotting for detection of <t>USP7,</t> TRIM24, and SPLUNC1 levels in THP-1-derived Mφs after transfected with shUSP7 or shNC for 48 h. B M1 (CD86 + CD11b + ) macrophages in THP-1-derived Mφs transfected with shUSP7 or shNC was determined by flow cytometry. C Co-IP assay for confirming the protein interaction between USP7 and TRIM24 in THP-1-derived Mφs. D The protein expression of USP7 and TRIM24 in THP-1-derived Mφs after transfection with USP7 overexpression plasmid was assessed by Western blotting. E , F TRIM24 protein level in USP7-depleted or overexpressed THP-1-derived Mφs with or without MG132 treatment for 12 h was measured by Western blotting. G The degradation of TRIM24 protein in THP-1-derived Mφs after administration with CHX for 15, 30, 60, 120, 240 min was evaluated by Western blotting. H HEK293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids. The deubiquitination of USP7 on TRIM24 in HEK293T cells was detected by Co-IP assay. I HEK 293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids and treated with or without USP7 inhibitors P5091(4 μM) or GNE6640 (0.75 μM). The ubiquitination level of TRIM24 in THP-1-derived Mφs was evaluated by Co-IP assay. * p < 0.05, ** p < 0.01, *** p < 0.001. For ( D – G ) Student’s t -test was performed. For ( A , B – E , F ) one-way ANOVA followed by Tukey’s multiple comparison test was performed.
Usp7, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/usp7/product/MedChemExpress
Average 93 stars, based on 1 article reviews
usp7 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
csnk1  (MedChemExpress)
90
MedChemExpress csnk1
A Western blotting for detection of <t>USP7,</t> TRIM24, and SPLUNC1 levels in THP-1-derived Mφs after transfected with shUSP7 or shNC for 48 h. B M1 (CD86 + CD11b + ) macrophages in THP-1-derived Mφs transfected with shUSP7 or shNC was determined by flow cytometry. C Co-IP assay for confirming the protein interaction between USP7 and TRIM24 in THP-1-derived Mφs. D The protein expression of USP7 and TRIM24 in THP-1-derived Mφs after transfection with USP7 overexpression plasmid was assessed by Western blotting. E , F TRIM24 protein level in USP7-depleted or overexpressed THP-1-derived Mφs with or without MG132 treatment for 12 h was measured by Western blotting. G The degradation of TRIM24 protein in THP-1-derived Mφs after administration with CHX for 15, 30, 60, 120, 240 min was evaluated by Western blotting. H HEK293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids. The deubiquitination of USP7 on TRIM24 in HEK293T cells was detected by Co-IP assay. I HEK 293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids and treated with or without USP7 inhibitors P5091(4 μM) or GNE6640 (0.75 μM). The ubiquitination level of TRIM24 in THP-1-derived Mφs was evaluated by Co-IP assay. * p < 0.05, ** p < 0.01, *** p < 0.001. For ( D – G ) Student’s t -test was performed. For ( A , B – E , F ) one-way ANOVA followed by Tukey’s multiple comparison test was performed.
Csnk1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/csnk1/product/MedChemExpress
Average 90 stars, based on 1 article reviews
csnk1 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


A Western blotting for detection of USP7, TRIM24, and SPLUNC1 levels in THP-1-derived Mφs after transfected with shUSP7 or shNC for 48 h. B M1 (CD86 + CD11b + ) macrophages in THP-1-derived Mφs transfected with shUSP7 or shNC was determined by flow cytometry. C Co-IP assay for confirming the protein interaction between USP7 and TRIM24 in THP-1-derived Mφs. D The protein expression of USP7 and TRIM24 in THP-1-derived Mφs after transfection with USP7 overexpression plasmid was assessed by Western blotting. E , F TRIM24 protein level in USP7-depleted or overexpressed THP-1-derived Mφs with or without MG132 treatment for 12 h was measured by Western blotting. G The degradation of TRIM24 protein in THP-1-derived Mφs after administration with CHX for 15, 30, 60, 120, 240 min was evaluated by Western blotting. H HEK293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids. The deubiquitination of USP7 on TRIM24 in HEK293T cells was detected by Co-IP assay. I HEK 293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids and treated with or without USP7 inhibitors P5091(4 μM) or GNE6640 (0.75 μM). The ubiquitination level of TRIM24 in THP-1-derived Mφs was evaluated by Co-IP assay. * p < 0.05, ** p < 0.01, *** p < 0.001. For ( D – G ) Student’s t -test was performed. For ( A , B – E , F ) one-way ANOVA followed by Tukey’s multiple comparison test was performed.

Journal: Cell Death & Disease

Article Title: USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24

doi: 10.1038/s41419-023-06368-w

Figure Lengend Snippet: A Western blotting for detection of USP7, TRIM24, and SPLUNC1 levels in THP-1-derived Mφs after transfected with shUSP7 or shNC for 48 h. B M1 (CD86 + CD11b + ) macrophages in THP-1-derived Mφs transfected with shUSP7 or shNC was determined by flow cytometry. C Co-IP assay for confirming the protein interaction between USP7 and TRIM24 in THP-1-derived Mφs. D The protein expression of USP7 and TRIM24 in THP-1-derived Mφs after transfection with USP7 overexpression plasmid was assessed by Western blotting. E , F TRIM24 protein level in USP7-depleted or overexpressed THP-1-derived Mφs with or without MG132 treatment for 12 h was measured by Western blotting. G The degradation of TRIM24 protein in THP-1-derived Mφs after administration with CHX for 15, 30, 60, 120, 240 min was evaluated by Western blotting. H HEK293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids. The deubiquitination of USP7 on TRIM24 in HEK293T cells was detected by Co-IP assay. I HEK 293T cells were transfected with HA-Ub, Myc-TRIM24, or Flag-USP7 plasmids and treated with or without USP7 inhibitors P5091(4 μM) or GNE6640 (0.75 μM). The ubiquitination level of TRIM24 in THP-1-derived Mφs was evaluated by Co-IP assay. * p < 0.05, ** p < 0.01, *** p < 0.001. For ( D – G ) Student’s t -test was performed. For ( A , B – E , F ) one-way ANOVA followed by Tukey’s multiple comparison test was performed.

Article Snippet: P5091(HY-15667, MedChemExpress) or GNE6640 (HY-112937, MedChemExpress) were used to impede the USP7.

Techniques: Western Blot, Derivative Assay, Transfection, Flow Cytometry, Co-Immunoprecipitation Assay, Expressing, Over Expression, Plasmid Preparation, Comparison

THP-1-derived Mφs were transfected with USP7 overexpression plasmid, shTRIM24, or a combination of them. A Protein abundance of USP7, TRIM24, and SPLUNC1 was evaluated by Western blotting. B Percentage of M1 (CD86 + CD11b + ) in THP-1-derived Mφs was assessed by flow cytometry. NPC/NK-1 and CNE3 cells were treated with CM collected from THP-1-derived Mφs transfected with USP7 overexpression plasmid, shTRIM24, or USP7 overexpression plasmid+ shTRIM24. C , D CCK-8 and colony formation assay for evaluation of the growth of NPC/NK-1 and CNE3 cells treated with CM from different groups. E The migratory capacity of NPC cells (NPC/NK-1 and CNE3) was analyzed by Transwell assay. Scale bar = 100 μm. * p < 0.05, ** p < 0.01, *** p < 0.001. One-way ANOVA followed by Tukey’s multiple comparison test was performed.

Journal: Cell Death & Disease

Article Title: USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24

doi: 10.1038/s41419-023-06368-w

Figure Lengend Snippet: THP-1-derived Mφs were transfected with USP7 overexpression plasmid, shTRIM24, or a combination of them. A Protein abundance of USP7, TRIM24, and SPLUNC1 was evaluated by Western blotting. B Percentage of M1 (CD86 + CD11b + ) in THP-1-derived Mφs was assessed by flow cytometry. NPC/NK-1 and CNE3 cells were treated with CM collected from THP-1-derived Mφs transfected with USP7 overexpression plasmid, shTRIM24, or USP7 overexpression plasmid+ shTRIM24. C , D CCK-8 and colony formation assay for evaluation of the growth of NPC/NK-1 and CNE3 cells treated with CM from different groups. E The migratory capacity of NPC cells (NPC/NK-1 and CNE3) was analyzed by Transwell assay. Scale bar = 100 μm. * p < 0.05, ** p < 0.01, *** p < 0.001. One-way ANOVA followed by Tukey’s multiple comparison test was performed.

Article Snippet: P5091(HY-15667, MedChemExpress) or GNE6640 (HY-112937, MedChemExpress) were used to impede the USP7.

Techniques: Derivative Assay, Transfection, Over Expression, Plasmid Preparation, Western Blot, Flow Cytometry, CCK-8 Assay, Colony Assay, Transwell Assay, Comparison

Nude mice were subcutaneously injected with NPC/NK-1 and CNE3 cells that were suspended in PBS or CM from THP-1-derived Mφs transfected with shTRIM24, USP7 overexpression plasmid or shTRIM24 + USP7 overexpression plasmid. A Representative images of tumors, ( B ) tumor growth, ( C ) tumor weight in nude mice. D Immunohistochemical staining of Ki67, TRIM24, USP7 and SPLUNC1 expression in tumor sections. Scale bar = 50 μm. Nude mice were injection with NPC cells (NPC/NK-1 and CNE3) resuspended in PBS or CM from THP-1-derived Mφs with various transfections by tail vein. E Representative images of lungs of nude mice 2 months after injection. Scale bar = 1 cm. F , G HE staining for observing metastatic lesions in lung tissues and the numbers of metastatic lesions were quantified. Scale bar = 50 μm. * p < 0.05, ** p < 0.01, *** p < 0.001. One-way ANOVA followed by Tukey’s multiple comparison test was performed.

Journal: Cell Death & Disease

Article Title: USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24

doi: 10.1038/s41419-023-06368-w

Figure Lengend Snippet: Nude mice were subcutaneously injected with NPC/NK-1 and CNE3 cells that were suspended in PBS or CM from THP-1-derived Mφs transfected with shTRIM24, USP7 overexpression plasmid or shTRIM24 + USP7 overexpression plasmid. A Representative images of tumors, ( B ) tumor growth, ( C ) tumor weight in nude mice. D Immunohistochemical staining of Ki67, TRIM24, USP7 and SPLUNC1 expression in tumor sections. Scale bar = 50 μm. Nude mice were injection with NPC cells (NPC/NK-1 and CNE3) resuspended in PBS or CM from THP-1-derived Mφs with various transfections by tail vein. E Representative images of lungs of nude mice 2 months after injection. Scale bar = 1 cm. F , G HE staining for observing metastatic lesions in lung tissues and the numbers of metastatic lesions were quantified. Scale bar = 50 μm. * p < 0.05, ** p < 0.01, *** p < 0.001. One-way ANOVA followed by Tukey’s multiple comparison test was performed.

Article Snippet: P5091(HY-15667, MedChemExpress) or GNE6640 (HY-112937, MedChemExpress) were used to impede the USP7.

Techniques: Injection, Derivative Assay, Transfection, Over Expression, Plasmid Preparation, Immunohistochemical staining, Staining, Expressing, Comparison

USP7 promotes the polarization of macrophages to M1 phenotype through de-ubiquitination and stabilization of TRIM24 that recruits STAT3 to trigger SPLUNC1 transcription, thereby repressing NPC cell growth and metastasis.

Journal: Cell Death & Disease

Article Title: USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24

doi: 10.1038/s41419-023-06368-w

Figure Lengend Snippet: USP7 promotes the polarization of macrophages to M1 phenotype through de-ubiquitination and stabilization of TRIM24 that recruits STAT3 to trigger SPLUNC1 transcription, thereby repressing NPC cell growth and metastasis.

Article Snippet: P5091(HY-15667, MedChemExpress) or GNE6640 (HY-112937, MedChemExpress) were used to impede the USP7.

Techniques: