HY-146691 Search Results


cjj300  (MedChemExpress)
91
MedChemExpress cjj300
SP1 in M2 macrophages activates fibroblasts via TGF-β1 (A) Transwell assay in which M2 macrophages were co-cultured with ligamentum flavum fibroblasts (LGF), lung fibroblasts (LF), and skin fibroblasts (SF) and the migration of the fibroblasts was measured. As controls, LGF, LF, and SF were cultured on their own. Photomicrographs were taken after 4 days of co-culture, followed by staining with crystal violet. Scale bar, 50 μm. N = 3. (B) Proliferation of LGF was evaluated at indicated time points (0, 2, and 4 days) in monoculture and co-culture with M2 macrophages using the Cell Counting Kit-8, with detection performed at 450 OD. Each experiment was independently repeated three times. (C) Immunostaining of LGF for the fibroblast markers vimentin and collagen I and III, monoculture or co-culture with M2 macrophages. Quantitative analysis is on the right. Scale bar, 200 μm. N = 3. (D) Concentration of collagen I and III in the medium of cultures of LGF alone or together with M2 macrophages. N = 4. (E) Levels of protein Smad4, Smad2/3, p-Smad2/3, and Smad7 in LGF, LF, and SF after 4 days in monoculture or co-culture with M2 macrophages. Quantitative analysis of western blots is below. N = 3. (F) Levels of protein SP1 and pro-TGF-β1 in M2 macrophages after 4 days in monoculture or co-culture with LGF, LF, and SF, respectively. Quantitative analysis of western blots is below. N = 3. (G) Levels of SP1 and pro-TGF-β1 in M2 macrophages that had been transfected and treated as described after 4 days co-culture with LGF. Quantitative analysis of western blots is below. N = 3. (H) Proliferation of LGF was evaluated at indicated time points (0, 2, and 4 days) in co-culture with M2 macrophages that had been transfected and treated as described using the Cell Counting Kit-8, with detection performed at 450 OD. Each experiment was independently repeated three times. (I) Concentration of collagen I and III in the medium of cultures of LGF together with M2 macrophages that had been transfected and treated as described. N = 3. KRFK (50 μM) alone or together with <t>CJJ300</t> (40 μM) was added to the co-culture medium of M2 macrophages and fibroblasts. Ligamentum flavum fibroblasts (LGF), lung fibroblasts (LF), and skin fibroblasts (SF). Data are mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Cjj300, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cjj300/product/MedChemExpress
Average 91 stars, based on 1 article reviews
cjj300 - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier

Image Search Results


SP1 in M2 macrophages activates fibroblasts via TGF-β1 (A) Transwell assay in which M2 macrophages were co-cultured with ligamentum flavum fibroblasts (LGF), lung fibroblasts (LF), and skin fibroblasts (SF) and the migration of the fibroblasts was measured. As controls, LGF, LF, and SF were cultured on their own. Photomicrographs were taken after 4 days of co-culture, followed by staining with crystal violet. Scale bar, 50 μm. N = 3. (B) Proliferation of LGF was evaluated at indicated time points (0, 2, and 4 days) in monoculture and co-culture with M2 macrophages using the Cell Counting Kit-8, with detection performed at 450 OD. Each experiment was independently repeated three times. (C) Immunostaining of LGF for the fibroblast markers vimentin and collagen I and III, monoculture or co-culture with M2 macrophages. Quantitative analysis is on the right. Scale bar, 200 μm. N = 3. (D) Concentration of collagen I and III in the medium of cultures of LGF alone or together with M2 macrophages. N = 4. (E) Levels of protein Smad4, Smad2/3, p-Smad2/3, and Smad7 in LGF, LF, and SF after 4 days in monoculture or co-culture with M2 macrophages. Quantitative analysis of western blots is below. N = 3. (F) Levels of protein SP1 and pro-TGF-β1 in M2 macrophages after 4 days in monoculture or co-culture with LGF, LF, and SF, respectively. Quantitative analysis of western blots is below. N = 3. (G) Levels of SP1 and pro-TGF-β1 in M2 macrophages that had been transfected and treated as described after 4 days co-culture with LGF. Quantitative analysis of western blots is below. N = 3. (H) Proliferation of LGF was evaluated at indicated time points (0, 2, and 4 days) in co-culture with M2 macrophages that had been transfected and treated as described using the Cell Counting Kit-8, with detection performed at 450 OD. Each experiment was independently repeated three times. (I) Concentration of collagen I and III in the medium of cultures of LGF together with M2 macrophages that had been transfected and treated as described. N = 3. KRFK (50 μM) alone or together with CJJ300 (40 μM) was added to the co-culture medium of M2 macrophages and fibroblasts. Ligamentum flavum fibroblasts (LGF), lung fibroblasts (LF), and skin fibroblasts (SF). Data are mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Journal: iScience

Article Title: Profibrotic role of transcription factor SP1 in cross-talk between fibroblasts and M2 macrophages

doi: 10.1016/j.isci.2023.108484

Figure Lengend Snippet: SP1 in M2 macrophages activates fibroblasts via TGF-β1 (A) Transwell assay in which M2 macrophages were co-cultured with ligamentum flavum fibroblasts (LGF), lung fibroblasts (LF), and skin fibroblasts (SF) and the migration of the fibroblasts was measured. As controls, LGF, LF, and SF were cultured on their own. Photomicrographs were taken after 4 days of co-culture, followed by staining with crystal violet. Scale bar, 50 μm. N = 3. (B) Proliferation of LGF was evaluated at indicated time points (0, 2, and 4 days) in monoculture and co-culture with M2 macrophages using the Cell Counting Kit-8, with detection performed at 450 OD. Each experiment was independently repeated three times. (C) Immunostaining of LGF for the fibroblast markers vimentin and collagen I and III, monoculture or co-culture with M2 macrophages. Quantitative analysis is on the right. Scale bar, 200 μm. N = 3. (D) Concentration of collagen I and III in the medium of cultures of LGF alone or together with M2 macrophages. N = 4. (E) Levels of protein Smad4, Smad2/3, p-Smad2/3, and Smad7 in LGF, LF, and SF after 4 days in monoculture or co-culture with M2 macrophages. Quantitative analysis of western blots is below. N = 3. (F) Levels of protein SP1 and pro-TGF-β1 in M2 macrophages after 4 days in monoculture or co-culture with LGF, LF, and SF, respectively. Quantitative analysis of western blots is below. N = 3. (G) Levels of SP1 and pro-TGF-β1 in M2 macrophages that had been transfected and treated as described after 4 days co-culture with LGF. Quantitative analysis of western blots is below. N = 3. (H) Proliferation of LGF was evaluated at indicated time points (0, 2, and 4 days) in co-culture with M2 macrophages that had been transfected and treated as described using the Cell Counting Kit-8, with detection performed at 450 OD. Each experiment was independently repeated three times. (I) Concentration of collagen I and III in the medium of cultures of LGF together with M2 macrophages that had been transfected and treated as described. N = 3. KRFK (50 μM) alone or together with CJJ300 (40 μM) was added to the co-culture medium of M2 macrophages and fibroblasts. Ligamentum flavum fibroblasts (LGF), lung fibroblasts (LF), and skin fibroblasts (SF). Data are mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: In some experiments, macrophages were treated with the KRFK (50 μM, cat# 162290-78-0, MedChemExpress, Shanghai, China), and fibroblasts were treated with CJJ300 (40 μM, cat# HY-146693, MedChemExpress, Shanghai, China).

Techniques: Transwell Assay, Cell Culture, Migration, Co-Culture Assay, Staining, Cell Counting, Immunostaining, Concentration Assay, Western Blot, Transfection