HY-146324 Search Results


ano1  (MedChemExpress)
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MedChemExpress ano1
Expression of LRRC8A in M 2 -MACs. ( A ) Real-time PCR examination of LRRC8A and <t>ANO1</t> in M 2 -MACs. Expression levels are shown as the ratio to the ACTB ( n = 4 for each). ( B ) Protein expression of LRRC8A (a) and ANO1 (b) in M 2 -MACs protein lysates. Blots were probed with anti-LRRC8A (approx. 95 kDa), anti-ANO1 (approx. 115 kDa), and anti-ACTB (approx. 45 kDa) antibodies. ( C ) Confocal fluorescence images (green) of Alexa Fluor 488-labeled LRRC8A (b) and ANO1 (c) and negative control with Alexa Fluor 488 alone (a) in M 2 -MACs. Dashed lines show cell boundaries. The scale bar in ‘(a)’ shows 10 μm (same in (b,c)).
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Expression of LRRC8A in M 2 -MACs. ( A ) Real-time PCR examination of LRRC8A and ANO1 in M 2 -MACs. Expression levels are shown as the ratio to the ACTB ( n = 4 for each). ( B ) Protein expression of LRRC8A (a) and ANO1 (b) in M 2 -MACs protein lysates. Blots were probed with anti-LRRC8A (approx. 95 kDa), anti-ANO1 (approx. 115 kDa), and anti-ACTB (approx. 45 kDa) antibodies. ( C ) Confocal fluorescence images (green) of Alexa Fluor 488-labeled LRRC8A (b) and ANO1 (c) and negative control with Alexa Fluor 488 alone (a) in M 2 -MACs. Dashed lines show cell boundaries. The scale bar in ‘(a)’ shows 10 μm (same in (b,c)).

Journal: International Journal of Molecular Sciences

Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages

doi: 10.3390/ijms25179612

Figure Lengend Snippet: Expression of LRRC8A in M 2 -MACs. ( A ) Real-time PCR examination of LRRC8A and ANO1 in M 2 -MACs. Expression levels are shown as the ratio to the ACTB ( n = 4 for each). ( B ) Protein expression of LRRC8A (a) and ANO1 (b) in M 2 -MACs protein lysates. Blots were probed with anti-LRRC8A (approx. 95 kDa), anti-ANO1 (approx. 115 kDa), and anti-ACTB (approx. 45 kDa) antibodies. ( C ) Confocal fluorescence images (green) of Alexa Fluor 488-labeled LRRC8A (b) and ANO1 (c) and negative control with Alexa Fluor 488 alone (a) in M 2 -MACs. Dashed lines show cell boundaries. The scale bar in ‘(a)’ shows 10 μm (same in (b,c)).

Article Snippet: EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Fluorescence, Labeling, Negative Control

Functional expression of LRRC8A in M 2 -MACs. ( A , B ) Simultaneous measurement of changes in membrane potential ( A ) and [Ca 2+ ] i ( B ) following the application of the LRRC8/ANO1 inhibitor, endovion (EDV, 1 μM), using bis-(1,3-dibutylbarbituric acid)trimethine oxonol [DiBAC 4 (3)] and Fura 2-acetoxymethyl ester (Fura 2-AM), respectively. The relative time course of changes in fluorescence intensities (1.0 at time 0 s) from an M 2 -MAC is shown. ( C , D ) Summarized results of EDV (1 μM)-induced depolarization (at 1 min) ( C ) and hyperpolarization (at 10 min) ( D ) responses. ( E , F ) Summarized results of EDV (1 μM)-induced changes in [Ca 2+ ] i at 1 min ( E ) and 10 min ( F ). ( G , H ) Measurement of changes in membrane potential following the application of the selective ANO1 inhibitor, ANO1-IN-1 (1 μM), using DiBAC 4 (3). The relative time course of changes in fluorescence intensities (1.0 at time 0 s) from an M 2 -MAC is shown ( G ). Summarized results of ANO1-IN-1-induced responses ( H ). Numbers used for experiments are shown in parentheses. **: p < 0.01 vs. the vehicle control.

Journal: International Journal of Molecular Sciences

Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages

doi: 10.3390/ijms25179612

Figure Lengend Snippet: Functional expression of LRRC8A in M 2 -MACs. ( A , B ) Simultaneous measurement of changes in membrane potential ( A ) and [Ca 2+ ] i ( B ) following the application of the LRRC8/ANO1 inhibitor, endovion (EDV, 1 μM), using bis-(1,3-dibutylbarbituric acid)trimethine oxonol [DiBAC 4 (3)] and Fura 2-acetoxymethyl ester (Fura 2-AM), respectively. The relative time course of changes in fluorescence intensities (1.0 at time 0 s) from an M 2 -MAC is shown. ( C , D ) Summarized results of EDV (1 μM)-induced depolarization (at 1 min) ( C ) and hyperpolarization (at 10 min) ( D ) responses. ( E , F ) Summarized results of EDV (1 μM)-induced changes in [Ca 2+ ] i at 1 min ( E ) and 10 min ( F ). ( G , H ) Measurement of changes in membrane potential following the application of the selective ANO1 inhibitor, ANO1-IN-1 (1 μM), using DiBAC 4 (3). The relative time course of changes in fluorescence intensities (1.0 at time 0 s) from an M 2 -MAC is shown ( G ). Summarized results of ANO1-IN-1-induced responses ( H ). Numbers used for experiments are shown in parentheses. **: p < 0.01 vs. the vehicle control.

Article Snippet: EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Functional Assay, Expressing, Membrane, Fluorescence, Control