HY-145195 Search Results


mtx  (MedChemExpress)
94
MedChemExpress mtx
NIC (NO donor) activated Wnt/β-catenin signaling, inhibited <t>MTX-induced</t> AML12 cell apoptosis, and promoted cell proliferation, <t>while</t> <t>MSAB</t> (β-catenin inhibitor) reversed the protective effect. (A) Morphology of AML12 cells in the control, MTX, MTX + NIC, and MTX + NIC + MSAB groups (scale bar = 200 μm). (B) CCK-8 cell viability assay results. (C-E) Relative mRNA levels of Bclxl , Bcl2 and Bax . (F) Immunofluorescence staining of Ki67 and DAPI (scale bar = 80 μm). (G) Quantitative score of Ki67 immunofluorescence staining. (H-K) Relative mRNA levels of Fzd6 , Axin2 , Myc and Ccnd1 . (L) Schematic diagram of the mechanism. Dietary nitrate up-regulated Fzd6 expression which enabling β-catenin accumulation and nuclear translocation. In the nucleus, β-catenin combines with Tcf/Lef, promoting the expression of c-Myc and cyclin D1, which is involved in cell proliferation. Data are expressed as mean ± SD, * , P <0.05; * * , P <0.01; * * * , P <0.001. MTX, methotrexate; MSAB, methyl 3-{[(4-methylphenyl) sulfonyl] amino}; CCK-8, cell counting kit-8; Fzd6, frizzled class receptor 6; Ccnd1, cyclin D1; Axin2, axin 2; Myc, myelocytomatosis oncogene; Tcf, transcription factor; Lef, lymphoid enhancer binding factor; Ki67, Kiel 67; DAPI, 4′,6-diamidino-2-phenylindole.
Mtx, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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methotrexate hydrate  (MedChemExpress)
95
MedChemExpress methotrexate hydrate
NIC (NO donor) activated Wnt/β-catenin signaling, inhibited <t>MTX-induced</t> AML12 cell apoptosis, and promoted cell proliferation, <t>while</t> <t>MSAB</t> (β-catenin inhibitor) reversed the protective effect. (A) Morphology of AML12 cells in the control, MTX, MTX + NIC, and MTX + NIC + MSAB groups (scale bar = 200 μm). (B) CCK-8 cell viability assay results. (C-E) Relative mRNA levels of Bclxl , Bcl2 and Bax . (F) Immunofluorescence staining of Ki67 and DAPI (scale bar = 80 μm). (G) Quantitative score of Ki67 immunofluorescence staining. (H-K) Relative mRNA levels of Fzd6 , Axin2 , Myc and Ccnd1 . (L) Schematic diagram of the mechanism. Dietary nitrate up-regulated Fzd6 expression which enabling β-catenin accumulation and nuclear translocation. In the nucleus, β-catenin combines with Tcf/Lef, promoting the expression of c-Myc and cyclin D1, which is involved in cell proliferation. Data are expressed as mean ± SD, * , P <0.05; * * , P <0.01; * * * , P <0.001. MTX, methotrexate; MSAB, methyl 3-{[(4-methylphenyl) sulfonyl] amino}; CCK-8, cell counting kit-8; Fzd6, frizzled class receptor 6; Ccnd1, cyclin D1; Axin2, axin 2; Myc, myelocytomatosis oncogene; Tcf, transcription factor; Lef, lymphoid enhancer binding factor; Ki67, Kiel 67; DAPI, 4′,6-diamidino-2-phenylindole.
Methotrexate Hydrate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
methotrexate hydrate - by Bioz Stars, 2026-02
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methotrexate disodium  (MedChemExpress)
93
MedChemExpress methotrexate disodium
NIC (NO donor) activated Wnt/β-catenin signaling, inhibited <t>MTX-induced</t> AML12 cell apoptosis, and promoted cell proliferation, <t>while</t> <t>MSAB</t> (β-catenin inhibitor) reversed the protective effect. (A) Morphology of AML12 cells in the control, MTX, MTX + NIC, and MTX + NIC + MSAB groups (scale bar = 200 μm). (B) CCK-8 cell viability assay results. (C-E) Relative mRNA levels of Bclxl , Bcl2 and Bax . (F) Immunofluorescence staining of Ki67 and DAPI (scale bar = 80 μm). (G) Quantitative score of Ki67 immunofluorescence staining. (H-K) Relative mRNA levels of Fzd6 , Axin2 , Myc and Ccnd1 . (L) Schematic diagram of the mechanism. Dietary nitrate up-regulated Fzd6 expression which enabling β-catenin accumulation and nuclear translocation. In the nucleus, β-catenin combines with Tcf/Lef, promoting the expression of c-Myc and cyclin D1, which is involved in cell proliferation. Data are expressed as mean ± SD, * , P <0.05; * * , P <0.01; * * * , P <0.001. MTX, methotrexate; MSAB, methyl 3-{[(4-methylphenyl) sulfonyl] amino}; CCK-8, cell counting kit-8; Fzd6, frizzled class receptor 6; Ccnd1, cyclin D1; Axin2, axin 2; Myc, myelocytomatosis oncogene; Tcf, transcription factor; Lef, lymphoid enhancer binding factor; Ki67, Kiel 67; DAPI, 4′,6-diamidino-2-phenylindole.
Methotrexate Disodium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methotrexate disodium - by Bioz Stars, 2026-02
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mrgprx2 antagonist 1  (MedChemExpress)
93
MedChemExpress mrgprx2 antagonist 1
Chitin inhibited <t>MRGPRX2‐mediated</t> LAD2 cells degranulation reaction. (A) Chitin showed little cytotoxicity on LAD2 cells analyzed by Abbkine‐Cell Counting Kit assays. (B and C) Chitin did not induce histamine and β‐hexosaminidase analyzed by ELISA. (D and E) Chitin inhibited MRGPRX2‐mediated LAD2 cells release histamine and β‐hexosaminidase in a dose‐dependent manner analyzed by ELISA. (F) The IC 50 value of chitin inhibited MRGPRX2‐mediated LAD2 cells release β‐hexosaminidase analyzed by ELISA. (Data expressed as mean ± SD. One‐way ANOVA was used, and the treated groups compared respectively with the negative control group).
Mrgprx2 Antagonist 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrgprx2 antagonist-5  (MedChemExpress)
N/A
MrgprX2 antagonist-5 is an MrgprX2 antagonist extracted from patent WO2020223255A1, example 16. MrgprX2 antagonist-5 can be used for the research of inflammatory disorders of the skin.
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Image Search Results


NIC (NO donor) activated Wnt/β-catenin signaling, inhibited MTX-induced AML12 cell apoptosis, and promoted cell proliferation, while MSAB (β-catenin inhibitor) reversed the protective effect. (A) Morphology of AML12 cells in the control, MTX, MTX + NIC, and MTX + NIC + MSAB groups (scale bar = 200 μm). (B) CCK-8 cell viability assay results. (C-E) Relative mRNA levels of Bclxl , Bcl2 and Bax . (F) Immunofluorescence staining of Ki67 and DAPI (scale bar = 80 μm). (G) Quantitative score of Ki67 immunofluorescence staining. (H-K) Relative mRNA levels of Fzd6 , Axin2 , Myc and Ccnd1 . (L) Schematic diagram of the mechanism. Dietary nitrate up-regulated Fzd6 expression which enabling β-catenin accumulation and nuclear translocation. In the nucleus, β-catenin combines with Tcf/Lef, promoting the expression of c-Myc and cyclin D1, which is involved in cell proliferation. Data are expressed as mean ± SD, * , P <0.05; * * , P <0.01; * * * , P <0.001. MTX, methotrexate; MSAB, methyl 3-{[(4-methylphenyl) sulfonyl] amino}; CCK-8, cell counting kit-8; Fzd6, frizzled class receptor 6; Ccnd1, cyclin D1; Axin2, axin 2; Myc, myelocytomatosis oncogene; Tcf, transcription factor; Lef, lymphoid enhancer binding factor; Ki67, Kiel 67; DAPI, 4′,6-diamidino-2-phenylindole.

Journal: Toxicology Research

Article Title: Nitrate protects against methotrexate-induced liver injury by activating Wnt/β-catenin Signaling in mice

doi: 10.1093/toxres/tfaf107

Figure Lengend Snippet: NIC (NO donor) activated Wnt/β-catenin signaling, inhibited MTX-induced AML12 cell apoptosis, and promoted cell proliferation, while MSAB (β-catenin inhibitor) reversed the protective effect. (A) Morphology of AML12 cells in the control, MTX, MTX + NIC, and MTX + NIC + MSAB groups (scale bar = 200 μm). (B) CCK-8 cell viability assay results. (C-E) Relative mRNA levels of Bclxl , Bcl2 and Bax . (F) Immunofluorescence staining of Ki67 and DAPI (scale bar = 80 μm). (G) Quantitative score of Ki67 immunofluorescence staining. (H-K) Relative mRNA levels of Fzd6 , Axin2 , Myc and Ccnd1 . (L) Schematic diagram of the mechanism. Dietary nitrate up-regulated Fzd6 expression which enabling β-catenin accumulation and nuclear translocation. In the nucleus, β-catenin combines with Tcf/Lef, promoting the expression of c-Myc and cyclin D1, which is involved in cell proliferation. Data are expressed as mean ± SD, * , P <0.05; * * , P <0.01; * * * , P <0.001. MTX, methotrexate; MSAB, methyl 3-{[(4-methylphenyl) sulfonyl] amino}; CCK-8, cell counting kit-8; Fzd6, frizzled class receptor 6; Ccnd1, cyclin D1; Axin2, axin 2; Myc, myelocytomatosis oncogene; Tcf, transcription factor; Lef, lymphoid enhancer binding factor; Ki67, Kiel 67; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: MTX (HY-14519, MedChemExpress, NJ, United States), MSAB (methyl 3-{[(4-methylphenyl) sulfonyl] amino} benzoate; HY-120697, MedChemExpress, NJ, United States) and NIC (HY-B0341, MedChemExpress, NJ, United States) were purchased from MedChemExpress.

Techniques: Control, CCK-8 Assay, Viability Assay, Immunofluorescence, Staining, Expressing, Translocation Assay, Cell Counting, Binding Assay

Chitin inhibited MRGPRX2‐mediated LAD2 cells degranulation reaction. (A) Chitin showed little cytotoxicity on LAD2 cells analyzed by Abbkine‐Cell Counting Kit assays. (B and C) Chitin did not induce histamine and β‐hexosaminidase analyzed by ELISA. (D and E) Chitin inhibited MRGPRX2‐mediated LAD2 cells release histamine and β‐hexosaminidase in a dose‐dependent manner analyzed by ELISA. (F) The IC 50 value of chitin inhibited MRGPRX2‐mediated LAD2 cells release β‐hexosaminidase analyzed by ELISA. (Data expressed as mean ± SD. One‐way ANOVA was used, and the treated groups compared respectively with the negative control group).

Journal: Food Science & Nutrition

Article Title: Evaluation of the Effects of Chitin and Chitosan on Pseudo‐Allergic Reaction by Inhibiting MRGPRX2 Activation

doi: 10.1002/fsn3.70877

Figure Lengend Snippet: Chitin inhibited MRGPRX2‐mediated LAD2 cells degranulation reaction. (A) Chitin showed little cytotoxicity on LAD2 cells analyzed by Abbkine‐Cell Counting Kit assays. (B and C) Chitin did not induce histamine and β‐hexosaminidase analyzed by ELISA. (D and E) Chitin inhibited MRGPRX2‐mediated LAD2 cells release histamine and β‐hexosaminidase in a dose‐dependent manner analyzed by ELISA. (F) The IC 50 value of chitin inhibited MRGPRX2‐mediated LAD2 cells release β‐hexosaminidase analyzed by ELISA. (Data expressed as mean ± SD. One‐way ANOVA was used, and the treated groups compared respectively with the negative control group).

Article Snippet: MrgprX2 antagonist‐1 was purchased from MCE (Shanghai, China, CAS No: 2642162‐06‐7).

Techniques: Cell Counting, Enzyme-linked Immunosorbent Assay, Negative Control

Chitosan inhibited MRGPRX2‐mediated LAD2 cells degranulation reaction. (A) Chitosan showed little cytotoxicity on LAD2 cells analyzed by Abbkine‐Cell Counting Kit assays. (B and C) Chitosan did not induce histamine and β‐hexosaminidase analyzed by ELISA. (D and E) Chitosan inhibited MRGPRX2‐mediated LAD2 cells release histamine and β‐hexosaminidase in a dose‐dependent manner analyzed by ELISA. (F) The IC 50 value of Chitosan inhibited MRGPRX2‐mediated LAD2 cells release β‐hexosaminidase analyzed by ELISA. (Data expressed as mean ± SD. One‐way ANOVA was used, and the treated groups compared respectively with the negative control group).

Journal: Food Science & Nutrition

Article Title: Evaluation of the Effects of Chitin and Chitosan on Pseudo‐Allergic Reaction by Inhibiting MRGPRX2 Activation

doi: 10.1002/fsn3.70877

Figure Lengend Snippet: Chitosan inhibited MRGPRX2‐mediated LAD2 cells degranulation reaction. (A) Chitosan showed little cytotoxicity on LAD2 cells analyzed by Abbkine‐Cell Counting Kit assays. (B and C) Chitosan did not induce histamine and β‐hexosaminidase analyzed by ELISA. (D and E) Chitosan inhibited MRGPRX2‐mediated LAD2 cells release histamine and β‐hexosaminidase in a dose‐dependent manner analyzed by ELISA. (F) The IC 50 value of Chitosan inhibited MRGPRX2‐mediated LAD2 cells release β‐hexosaminidase analyzed by ELISA. (Data expressed as mean ± SD. One‐way ANOVA was used, and the treated groups compared respectively with the negative control group).

Article Snippet: MrgprX2 antagonist‐1 was purchased from MCE (Shanghai, China, CAS No: 2642162‐06‐7).

Techniques: Cell Counting, Enzyme-linked Immunosorbent Assay, Negative Control

Chitin and chitosan inhibited MRGPRX2‐mediated LAD2 cells release of cytokines. (A) Chitin inhibited MRGPRX2‐mediated LAD2 cells release of TNF‐α, MCP‐1, and IL‐8 analyzed by ELISA. (B) Chitosan inhibited MRGPRX2‐mediated LAD2 cells release of TNF‐α, MCP‐1, and IL‐8 analyzed by ELISA. (Data expressed as mean ± SD. One‐way ANOVA was used, and the treated groups compared respectively with the negative control group).

Journal: Food Science & Nutrition

Article Title: Evaluation of the Effects of Chitin and Chitosan on Pseudo‐Allergic Reaction by Inhibiting MRGPRX2 Activation

doi: 10.1002/fsn3.70877

Figure Lengend Snippet: Chitin and chitosan inhibited MRGPRX2‐mediated LAD2 cells release of cytokines. (A) Chitin inhibited MRGPRX2‐mediated LAD2 cells release of TNF‐α, MCP‐1, and IL‐8 analyzed by ELISA. (B) Chitosan inhibited MRGPRX2‐mediated LAD2 cells release of TNF‐α, MCP‐1, and IL‐8 analyzed by ELISA. (Data expressed as mean ± SD. One‐way ANOVA was used, and the treated groups compared respectively with the negative control group).

Article Snippet: MrgprX2 antagonist‐1 was purchased from MCE (Shanghai, China, CAS No: 2642162‐06‐7).

Techniques: Enzyme-linked Immunosorbent Assay, Negative Control