HY-144991 Search Results


pi3kδ  (MedChemExpress)
93
MedChemExpress pi3kδ
( A ) Mixed WT and CRISPR KO dHL-60 populations post-stimulation show that CA2 (black) and PI3Ky (green) KO both fail to decrease their densities as much as the WT (cyan) population following chemoattractant stimulation. Cells with negative control guides (light grey) have normal volume responses. ( B ) <t>PI3Kδ/γ</t> inhibition completely blocks the chemoattractant-induced volume change in primary human neutrophils. ( C ) To validate the FxM results, primary human neutrophils were stimulated in suspension, and their volumes were measured using a Coulter counter (WT replicates = 4, NHE1 = 2). 20 nM fMLP was added at the 0 minute marker. ( D ) PI3Kδ/γ inhibition blocked the chemoattractant-drive shape change in human primary neutrophils, as measured by the change in footprint area in FxM ( E ) The coefficient of variation in volume for control (cyan) and iNHE1 (gold) human primary neutrophils undergoing chemokinesis are comparable, suggesting that the volume fluctuations are unchanged upon NHE1 inhibition despite the different baseline volumes.
Pi3kδ, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nvp dky709  (MedChemExpress)
94
MedChemExpress nvp dky709
( A ) Mixed WT and CRISPR KO dHL-60 populations post-stimulation show that CA2 (black) and PI3Ky (green) KO both fail to decrease their densities as much as the WT (cyan) population following chemoattractant stimulation. Cells with negative control guides (light grey) have normal volume responses. ( B ) <t>PI3Kδ/γ</t> inhibition completely blocks the chemoattractant-induced volume change in primary human neutrophils. ( C ) To validate the FxM results, primary human neutrophils were stimulated in suspension, and their volumes were measured using a Coulter counter (WT replicates = 4, NHE1 = 2). 20 nM fMLP was added at the 0 minute marker. ( D ) PI3Kδ/γ inhibition blocked the chemoattractant-drive shape change in human primary neutrophils, as measured by the change in footprint area in FxM ( E ) The coefficient of variation in volume for control (cyan) and iNHE1 (gold) human primary neutrophils undergoing chemokinesis are comparable, suggesting that the volume fluctuations are unchanged upon NHE1 inhibition despite the different baseline volumes.
Nvp Dky709, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
nvp dky709 - by Bioz Stars, 2026-03
94/100 stars
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aurora kinase inhibitor-8  (MedChemExpress)
N/A
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Image Search Results


( A ) Mixed WT and CRISPR KO dHL-60 populations post-stimulation show that CA2 (black) and PI3Ky (green) KO both fail to decrease their densities as much as the WT (cyan) population following chemoattractant stimulation. Cells with negative control guides (light grey) have normal volume responses. ( B ) PI3Kδ/γ inhibition completely blocks the chemoattractant-induced volume change in primary human neutrophils. ( C ) To validate the FxM results, primary human neutrophils were stimulated in suspension, and their volumes were measured using a Coulter counter (WT replicates = 4, NHE1 = 2). 20 nM fMLP was added at the 0 minute marker. ( D ) PI3Kδ/γ inhibition blocked the chemoattractant-drive shape change in human primary neutrophils, as measured by the change in footprint area in FxM ( E ) The coefficient of variation in volume for control (cyan) and iNHE1 (gold) human primary neutrophils undergoing chemokinesis are comparable, suggesting that the volume fluctuations are unchanged upon NHE1 inhibition despite the different baseline volumes.

Journal: bioRxiv

Article Title: Neutrophils actively swell to potentiate rapid migration

doi: 10.1101/2023.05.15.540704

Figure Lengend Snippet: ( A ) Mixed WT and CRISPR KO dHL-60 populations post-stimulation show that CA2 (black) and PI3Ky (green) KO both fail to decrease their densities as much as the WT (cyan) population following chemoattractant stimulation. Cells with negative control guides (light grey) have normal volume responses. ( B ) PI3Kδ/γ inhibition completely blocks the chemoattractant-induced volume change in primary human neutrophils. ( C ) To validate the FxM results, primary human neutrophils were stimulated in suspension, and their volumes were measured using a Coulter counter (WT replicates = 4, NHE1 = 2). 20 nM fMLP was added at the 0 minute marker. ( D ) PI3Kδ/γ inhibition blocked the chemoattractant-drive shape change in human primary neutrophils, as measured by the change in footprint area in FxM ( E ) The coefficient of variation in volume for control (cyan) and iNHE1 (gold) human primary neutrophils undergoing chemokinesis are comparable, suggesting that the volume fluctuations are unchanged upon NHE1 inhibition despite the different baseline volumes.

Article Snippet: For PI3Kδ/γ inhibition, Duvelisib (MedChemExpress #HY-17044) was stored at 10 mM in DMSO and used at a final concentration of 1 uM.

Techniques: CRISPR, Negative Control, Inhibition, Suspension, Marker, Control

( A ) Control neutrophils show an increased angular alignment upon stimulation as their motility becomes directional. NHE1-inhibition has very little effect on this process. ( B ) PI3Kδ/γ completely blocked the chemoattractant-induced motility increase in primary human neutrophils

Journal: bioRxiv

Article Title: Neutrophils actively swell to potentiate rapid migration

doi: 10.1101/2023.05.15.540704

Figure Lengend Snippet: ( A ) Control neutrophils show an increased angular alignment upon stimulation as their motility becomes directional. NHE1-inhibition has very little effect on this process. ( B ) PI3Kδ/γ completely blocked the chemoattractant-induced motility increase in primary human neutrophils

Article Snippet: For PI3Kδ/γ inhibition, Duvelisib (MedChemExpress #HY-17044) was stored at 10 mM in DMSO and used at a final concentration of 1 uM.

Techniques: Control, Inhibition