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MedChemExpress rimonabant
CB1 signaling contributes to BI-5756-mediated Treg upregulation. T cells were stimulated with anti-CD3/CD28 activation beads for 3 days in the presence or absence of BI-5756 (10 μM) and/or <t>rimonabant,</t> a CB1 antagonist, at the indicated concentrations. ( A ) The %CD4 and CD8, ( B ) normalized proportion of DP T cells, and ( C ) % Tregs were determined by flow cytometry. ( C , D ) Tregs were identified by GFP-positive cells within the CD4 or DP T cell population. * p < 0.05, ** p < 0.01, and *** p < 0.001. All error bars are represented as mean ± standard deviation.
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CB1 signaling contributes to BI-5756-mediated Treg upregulation. T cells were stimulated with anti-CD3/CD28 activation beads for 3 days in the presence or absence of BI-5756 (10 μM) and/or rimonabant, a CB1 antagonist, at the indicated concentrations. ( A ) The %CD4 and CD8, ( B ) normalized proportion of DP T cells, and ( C ) % Tregs were determined by flow cytometry. ( C , D ) Tregs were identified by GFP-positive cells within the CD4 or DP T cell population. * p < 0.05, ** p < 0.01, and *** p < 0.001. All error bars are represented as mean ± standard deviation.

Journal: Molecules

Article Title: BI-5756 Reduces Graft-Versus-Host Disease Through CB1-Mediated Treg Upregulation

doi: 10.3390/molecules30173517

Figure Lengend Snippet: CB1 signaling contributes to BI-5756-mediated Treg upregulation. T cells were stimulated with anti-CD3/CD28 activation beads for 3 days in the presence or absence of BI-5756 (10 μM) and/or rimonabant, a CB1 antagonist, at the indicated concentrations. ( A ) The %CD4 and CD8, ( B ) normalized proportion of DP T cells, and ( C ) % Tregs were determined by flow cytometry. ( C , D ) Tregs were identified by GFP-positive cells within the CD4 or DP T cell population. * p < 0.05, ** p < 0.01, and *** p < 0.001. All error bars are represented as mean ± standard deviation.

Article Snippet: Rimonabant (#HY-14137) and CB1 agonist 1 (#HY-148137) were obtained from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Activation Assay, Flow Cytometry, Standard Deviation