HY-13645 Search Results


eif4a inhibitor cr 1 31 b  (MedChemExpress)
94
MedChemExpress eif4a inhibitor cr 1 31 b
PDCD4 regulates the secretion of M2‐polarizing proteins in an <t>eIF4A‐dependent</t> manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Eif4a Inhibitor Cr 1 31 B, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eif4a inhibitor cr 1 31 b/product/MedChemExpress
Average 94 stars, based on 1 article reviews
eif4a inhibitor cr 1 31 b - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
cr-1-30-b  (MedChemExpress)
N/A
   Buy from Supplier
triclabendazole sulfoxide  (MedChemExpress)
N/A
Triclabendazole sulfoxide (TCBZ-SO) is the main plasma metabolite of Triclabendazole, and exhibits anti-parasite effects. Triclabendazole sulfoxide can inhibit membrane transporter ABCG2/BCRP.
   Buy from Supplier

Image Search Results


PDCD4 regulates the secretion of M2‐polarizing proteins in an eIF4A‐dependent manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Advanced Science

Article Title: Spatial Transcriptomics Reveals Transcriptomic and Immune Microenvironment Reprogramming during Thyroid Carcinoma Dedifferentiation

doi: 10.1002/advs.202506925

Figure Lengend Snippet: PDCD4 regulates the secretion of M2‐polarizing proteins in an eIF4A‐dependent manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The eIF4A inhibitor CR‐1‐31‐B (MCE, Cat#HY‐136453) was administered to mice at a dose of 2 mg kg −1 via intraperitoneal injection every other day in mouse drug administration experiments.

Techniques: Expressing, Co-Immunoprecipitation Assay, Knock-Out, Over Expression, Knockdown, Inhibition, In Vivo, Immunoprecipitation, Flow Cytometry, Western Blot, Comparison