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Extracellular matrix stiffness regulates fibroblast-to-myofibroblast transition through DNMT1 (a-c) Western blot and immunohistochemical staining of DNMT1 protein in the anterior vaginal wall of control and POP patients; (d,f) Expression of DNMT1 in cells cultured on gel with different stiffness.(e,f) The protein levels of these genes were detected by Western blot. Cells were treated with 5 ‘-AZA or PBS as control group and cultured on soft PA gel.
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Extracellular matrix stiffness regulates fibroblast-to-myofibroblast transition through DNMT1 (a-c) Western blot and immunohistochemical staining of DNMT1 protein in the anterior vaginal wall of control and POP patients; (d,f) Expression of DNMT1 in cells cultured on gel with different stiffness.(e,f) The protein levels of these genes were detected by Western blot. Cells were treated with 5 ‘-AZA or PBS as control group and cultured on soft PA gel.

Journal: bioRxiv

Article Title: Extracellular matrix stiffness regulates fibroblast differentiation by influencing DNA methyltransferase 1 expression through microtubule polymerization

doi: 10.1101/2022.10.20.513009

Figure Lengend Snippet: Extracellular matrix stiffness regulates fibroblast-to-myofibroblast transition through DNMT1 (a-c) Western blot and immunohistochemical staining of DNMT1 protein in the anterior vaginal wall of control and POP patients; (d,f) Expression of DNMT1 in cells cultured on gel with different stiffness.(e,f) The protein levels of these genes were detected by Western blot. Cells were treated with 5 ‘-AZA or PBS as control group and cultured on soft PA gel.

Article Snippet: Nocodazole and 5’-Aza were purchased from MedChemExpress (MCE).

Techniques: Western Blot, Immunohistochemical staining, Staining, Expressing, Cell Culture

Cells convert physical signals to biological signals through the microtubule cytoskeleton(a) Gene correlation analysis showed that DNMT1 expression was significantly correlated with genes related to microtubule polymerization; (b,c) The morphological differences of cells cultured with high stiffness gel and added with Nocodazole were observed under forward microscope.(d,e) The protein levels of these genes were detected by Western blot. Cells were treated with 5 ‘-AZA or PBS as control group and cultured on soft PA gel.

Journal: bioRxiv

Article Title: Extracellular matrix stiffness regulates fibroblast differentiation by influencing DNA methyltransferase 1 expression through microtubule polymerization

doi: 10.1101/2022.10.20.513009

Figure Lengend Snippet: Cells convert physical signals to biological signals through the microtubule cytoskeleton(a) Gene correlation analysis showed that DNMT1 expression was significantly correlated with genes related to microtubule polymerization; (b,c) The morphological differences of cells cultured with high stiffness gel and added with Nocodazole were observed under forward microscope.(d,e) The protein levels of these genes were detected by Western blot. Cells were treated with 5 ‘-AZA or PBS as control group and cultured on soft PA gel.

Article Snippet: Nocodazole and 5’-Aza were purchased from MedChemExpress (MCE).

Techniques: Expressing, Cell Culture, Microscopy, Western Blot