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sr144528  (MedChemExpress)
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MedChemExpress sr144528
a – d Mice were treated with celastrol (1 mg/kg) by intraperitoneal injection immediately after UUO surgery. The <t>celastrol+SR144528</t> group was additionally pretreated with SR144528 (1 mg/kg) 1 h before the celastrol injection. Both celastrol and SR144528 were administered daily. Mice were killed 7 days after UUO and kidney tissues were collected. a Representative micrographs show kidney injury using PAS staining and quantification of tubular injury scoring in different groups of mice. Bar = 100 μm. b Masson’s trichrome staining of kidney tissues sections. Bar = 50 μm. c Representative micrographs of the expression and distribution of α-SMA in kidney tissues using immunohistochemical staining. Bar = 100 μm. d Western blot analyses of renal fibronectin, collagen I, and α-SMA protein in kidney tissues. Representative western blot and quantitative data were presented. e Serum-starved HK-2 cells were pretreated with or without celastrol (500 nM) for 1 h and then stimulated with TGF-β1 (10 ng/ml) for 24 h. The celastrol+SR144528 group was additionally pretreated with SR144528 (1 μM) 30 min before the celastrol administration. Representative western blot analysis and quantification of fibronectin, collagen I, and α-SMA protein expression in HK-2 cells were presented. The medium group was used as the control for TGF-β1 treatment. Results are representative of three replicate experiments. All values are represented as mean ± SEM. n = 5 /group. * P < 0.05, ** P < 0.01, and *** P < 0.001
Sr144528, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a – d Mice were treated with celastrol (1 mg/kg) by intraperitoneal injection immediately after UUO surgery. The celastrol+SR144528 group was additionally pretreated with SR144528 (1 mg/kg) 1 h before the celastrol injection. Both celastrol and SR144528 were administered daily. Mice were killed 7 days after UUO and kidney tissues were collected. a Representative micrographs show kidney injury using PAS staining and quantification of tubular injury scoring in different groups of mice. Bar = 100 μm. b Masson’s trichrome staining of kidney tissues sections. Bar = 50 μm. c Representative micrographs of the expression and distribution of α-SMA in kidney tissues using immunohistochemical staining. Bar = 100 μm. d Western blot analyses of renal fibronectin, collagen I, and α-SMA protein in kidney tissues. Representative western blot and quantitative data were presented. e Serum-starved HK-2 cells were pretreated with or without celastrol (500 nM) for 1 h and then stimulated with TGF-β1 (10 ng/ml) for 24 h. The celastrol+SR144528 group was additionally pretreated with SR144528 (1 μM) 30 min before the celastrol administration. Representative western blot analysis and quantification of fibronectin, collagen I, and α-SMA protein expression in HK-2 cells were presented. The medium group was used as the control for TGF-β1 treatment. Results are representative of three replicate experiments. All values are represented as mean ± SEM. n = 5 /group. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Cell Death & Disease

Article Title: Celastrol alleviates renal fibrosis by upregulating cannabinoid receptor 2 expression

doi: 10.1038/s41419-018-0666-y

Figure Lengend Snippet: a – d Mice were treated with celastrol (1 mg/kg) by intraperitoneal injection immediately after UUO surgery. The celastrol+SR144528 group was additionally pretreated with SR144528 (1 mg/kg) 1 h before the celastrol injection. Both celastrol and SR144528 were administered daily. Mice were killed 7 days after UUO and kidney tissues were collected. a Representative micrographs show kidney injury using PAS staining and quantification of tubular injury scoring in different groups of mice. Bar = 100 μm. b Masson’s trichrome staining of kidney tissues sections. Bar = 50 μm. c Representative micrographs of the expression and distribution of α-SMA in kidney tissues using immunohistochemical staining. Bar = 100 μm. d Western blot analyses of renal fibronectin, collagen I, and α-SMA protein in kidney tissues. Representative western blot and quantitative data were presented. e Serum-starved HK-2 cells were pretreated with or without celastrol (500 nM) for 1 h and then stimulated with TGF-β1 (10 ng/ml) for 24 h. The celastrol+SR144528 group was additionally pretreated with SR144528 (1 μM) 30 min before the celastrol administration. Representative western blot analysis and quantification of fibronectin, collagen I, and α-SMA protein expression in HK-2 cells were presented. The medium group was used as the control for TGF-β1 treatment. Results are representative of three replicate experiments. All values are represented as mean ± SEM. n = 5 /group. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: For the in vitro studies, serum-starved HK-2 cells were preincubated with or without the indicated amount of celastrol, SR144528 (1 μM), or SIS3 (10 ng/ml; Medchemexpress, USA) for 1 h, followed by stimulation with recombinant murine TGF-β1 (10 ng/ml; R&D Systems, Minneapolis, MN, USA) for indicated time points.

Techniques: Injection, Staining, Expressing, Immunohistochemical staining, Western Blot, Control