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mp265  (MedChemExpress)
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MedChemExpress mp265
Wild-type cells were treated with 80 µg/mL <t>MP265</t> 10 min prior to and during 1-M osmotic shocks. The lack of coupling is similar to the effects of A22 treatment (Fig. 3e).
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Wild-type cells were treated with 80 µg/mL MP265 10 min prior to and during 1-M osmotic shocks. The lack of coupling is similar to the effects of A22 treatment (Fig. 3e).

Journal: bioRxiv

Article Title: A load-bearing function for the cytoplasmic membrane of Escherichia coli

doi: 10.1101/2025.10.02.680147

Figure Lengend Snippet: Wild-type cells were treated with 80 µg/mL MP265 10 min prior to and during 1-M osmotic shocks. The lack of coupling is similar to the effects of A22 treatment (Fig. 3e).

Article Snippet: Cells were first perfused with LB for 30 min with 50 μg/mL cephalexin (MP Biomedicals, Cat. #150585) to inhibit cell division and then treated with 100 μg/mL A22 (gift from Douglas Weibel), 80 μg/mL MP265 (MedChemExpress, Cat. #HY-131583), a beta-lactam antibiotic (100 μg/mL ampicillin (RPI, Cat. #A40040), 50 μg/mL mecillinam (Sigma-Aldrich, Cat. #33447), or 30 μg/mL cefsulodin (MP Biomedicals, Cat. #198677), or 0.85X PBS (Gibco, Cat. #70011044) for 10 min to perturb cell-wall synthesis.

Techniques: