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Image Search Results
Journal: Frontiers in pharmacology
Article Title: Uridine Diphosphate Promotes Rheumatoid Arthritis Through P2Y6 Activation.
doi: 10.3389/fphar.2021.658511
Figure Lengend Snippet: FIGURE 6 | The Effect of MRS2578 on cell activities and IL-6 production in cultured synovial fibroblast cells. The cultured synovial fibroblast cells of RA (n 5) were treated with UDP (100 μM) and/or MRS2578 (10 μM). (A) Cell proliferation was assessed using RTCA and statistical analysis. (B) Apoptosis was assessed using flow cytometry and statistical analysis. (C) IL-6 secretion was assessed using a flow cytometric bead assay and statistical analysis. (D) Cell migration was assessed using a wound healing assay and statistical analysis. (E) Cell migration was assessed using a Transwell assay and statistical analysis. These experiments were repeated three times. * FLS: synovial fibroblast cells. *p < 0.05, **p < 0.01 and ***p < 0.001.
Article Snippet: These rats were randomly divided into a PBS treatment group (containing 1% DMSO, n 9), a UDP treatment group (10 mg/kg, n 9), an
Techniques: Cell Culture, Cytometry, Migration, Wound Healing Assay, Transwell Assay
Journal: Frontiers in pharmacology
Article Title: Uridine Diphosphate Promotes Rheumatoid Arthritis Through P2Y6 Activation.
doi: 10.3389/fphar.2021.658511
Figure Lengend Snippet: FIGURE 7 | The effect of MRS2578 on CIA rats treated with UDP. (A) X-ray images and histochemical staining images of joint inflammation. (B) Disease scores were quantified based on histologic evidence. (C) Inflammation curve analysis based on paw inflammation. (D) Cytokine levels in synovial fluids using a flow cytometric bead assay. (E) IL-6 levels in synovial fluids were assessed using ELISA. X-ray images, joint tissues, peripheral blood and synovial fluids were collected 20 days after the first UDP injection (3 days after the last UDP injection/7 weeks after the first collagen injection). Each groups had nine rats, SF: synovial fluids. *p < 0.05, **p < 0.01 and ***p < 0.001.
Article Snippet: These rats were randomly divided into a PBS treatment group (containing 1% DMSO, n 9), a UDP treatment group (10 mg/kg, n 9), an
Techniques: Staining, Enzyme-linked Immunosorbent Assay, Injection