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pimozide  (MedChemExpress)
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MedChemExpress pimozide
The anti-proliferative effect of <t>pimozide</t> on liver cancer cells is not associated with cell <t>death.</t> <t>Sk-Hep1,</t> Huh7 and HepG2 cells were incubated with 5 and 10 µM pimozide for 48 h for cell viability (A) and colony formation (B) assays. (C) Apoptotic cells detected by Annexin V/PI staining. (D) Treatment of liver cancer cells with 10 µM pimozide alone or in combination with the pan-caspase inhibitor Z-VAD-FMK (10 µM) or the RIP kinase inhibitor necrostain 1 (10 µM) for 48 h before being subjected to cell viability assays. Representative images from one of three independent experiments are shown. Mean values are shown with SDs of three independent experiments; **P<0.01, compared to the control. NS, not significant.
Pimozide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The anti-proliferative effect of pimozide on liver cancer cells is not associated with cell death. Sk-Hep1, Huh7 and HepG2 cells were incubated with 5 and 10 µM pimozide for 48 h for cell viability (A) and colony formation (B) assays. (C) Apoptotic cells detected by Annexin V/PI staining. (D) Treatment of liver cancer cells with 10 µM pimozide alone or in combination with the pan-caspase inhibitor Z-VAD-FMK (10 µM) or the RIP kinase inhibitor necrostain 1 (10 µM) for 48 h before being subjected to cell viability assays. Representative images from one of three independent experiments are shown. Mean values are shown with SDs of three independent experiments; **P<0.01, compared to the control. NS, not significant.

Journal: Oncology Reports

Article Title: Antipsychotic agent pimozide promotes reversible proliferative suppression by inducing cellular quiescence in liver cancer

doi: 10.3892/or.2019.7229

Figure Lengend Snippet: The anti-proliferative effect of pimozide on liver cancer cells is not associated with cell death. Sk-Hep1, Huh7 and HepG2 cells were incubated with 5 and 10 µM pimozide for 48 h for cell viability (A) and colony formation (B) assays. (C) Apoptotic cells detected by Annexin V/PI staining. (D) Treatment of liver cancer cells with 10 µM pimozide alone or in combination with the pan-caspase inhibitor Z-VAD-FMK (10 µM) or the RIP kinase inhibitor necrostain 1 (10 µM) for 48 h before being subjected to cell viability assays. Representative images from one of three independent experiments are shown. Mean values are shown with SDs of three independent experiments; **P<0.01, compared to the control. NS, not significant.

Article Snippet: In addition, Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide alone or with combination plus pan-caspase inhibitor Z-VAD-FMK (10 µM) or RIP kinase inhibitor necrostain 1 (10 µM) or 2 mM NAC (MedChem Express) for 48 h before being subjected to cell viability assay.

Techniques: Incubation, Staining, Control

Pimozide induces G0/G1 phase cell cycle arrest in HCC cells. (A) Cell cycle phase distribution of PI-stained Sk-Hep1 and Huh7 cells following treatment with 10 µM pimozide was determined by flow cytometry. Representative images from one of three independent experiments are shown. (B) Western blot analysis of cell cycle-related gene expression following 0, 5, 10 and 15 µM pimozide. Cell extracts were probed with antibodies against CDK6, p-Rb, cyclin D1 and p27 proteins. β-actin was used as a loading control. HCC, hepatocellular carcinoma; CDK6, cyclin dependent kinase 6.

Journal: Oncology Reports

Article Title: Antipsychotic agent pimozide promotes reversible proliferative suppression by inducing cellular quiescence in liver cancer

doi: 10.3892/or.2019.7229

Figure Lengend Snippet: Pimozide induces G0/G1 phase cell cycle arrest in HCC cells. (A) Cell cycle phase distribution of PI-stained Sk-Hep1 and Huh7 cells following treatment with 10 µM pimozide was determined by flow cytometry. Representative images from one of three independent experiments are shown. (B) Western blot analysis of cell cycle-related gene expression following 0, 5, 10 and 15 µM pimozide. Cell extracts were probed with antibodies against CDK6, p-Rb, cyclin D1 and p27 proteins. β-actin was used as a loading control. HCC, hepatocellular carcinoma; CDK6, cyclin dependent kinase 6.

Article Snippet: In addition, Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide alone or with combination plus pan-caspase inhibitor Z-VAD-FMK (10 µM) or RIP kinase inhibitor necrostain 1 (10 µM) or 2 mM NAC (MedChem Express) for 48 h before being subjected to cell viability assay.

Techniques: Staining, Flow Cytometry, Western Blot, Expressing, Control

Pimozide induces reversible proliferative suppression in HCC cells. (A) Experimental design diagram: 5 µM pimozide, Sk-Hep1 and Huh7 cells were re-plated for cell proliferative and colony formation assays. Analyses of cell proliferative (B) and colony formation (C) assays of Sk-Hep1 and Huh7 cells after pimozide treatment. (D) For the colony formation assay, the numbers of crystal violet-stained colonies were counted. The results were from 3 independent experiments. HCC, hepatocellular carcinoma.

Journal: Oncology Reports

Article Title: Antipsychotic agent pimozide promotes reversible proliferative suppression by inducing cellular quiescence in liver cancer

doi: 10.3892/or.2019.7229

Figure Lengend Snippet: Pimozide induces reversible proliferative suppression in HCC cells. (A) Experimental design diagram: 5 µM pimozide, Sk-Hep1 and Huh7 cells were re-plated for cell proliferative and colony formation assays. Analyses of cell proliferative (B) and colony formation (C) assays of Sk-Hep1 and Huh7 cells after pimozide treatment. (D) For the colony formation assay, the numbers of crystal violet-stained colonies were counted. The results were from 3 independent experiments. HCC, hepatocellular carcinoma.

Article Snippet: In addition, Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide alone or with combination plus pan-caspase inhibitor Z-VAD-FMK (10 µM) or RIP kinase inhibitor necrostain 1 (10 µM) or 2 mM NAC (MedChem Express) for 48 h before being subjected to cell viability assay.

Techniques: Colony Assay, Staining

Pimozide induces cellular quiescence in HCC cells. (A) Sk-Hep1 and Huh7 cells were incubated with 0, 5 and 10 µM pimozide for 48 h and subjected to Ki-67-FITC/PI staining to detect quiescent (G0 phase) cells. Representative flow cytometry data illustrating the Ki67 − /PI − population gates for the G0 phase are shown with representative images from one of three independent experiments. (B) Western blot analysis of p-STAT3, STAT3, p-ERK1/2 and ERK1/2 in HCC cells after 0, 5, 10 and 15 µM pimozide treatment for 48 h. HCC, hepatocellular carcinoma.

Journal: Oncology Reports

Article Title: Antipsychotic agent pimozide promotes reversible proliferative suppression by inducing cellular quiescence in liver cancer

doi: 10.3892/or.2019.7229

Figure Lengend Snippet: Pimozide induces cellular quiescence in HCC cells. (A) Sk-Hep1 and Huh7 cells were incubated with 0, 5 and 10 µM pimozide for 48 h and subjected to Ki-67-FITC/PI staining to detect quiescent (G0 phase) cells. Representative flow cytometry data illustrating the Ki67 − /PI − population gates for the G0 phase are shown with representative images from one of three independent experiments. (B) Western blot analysis of p-STAT3, STAT3, p-ERK1/2 and ERK1/2 in HCC cells after 0, 5, 10 and 15 µM pimozide treatment for 48 h. HCC, hepatocellular carcinoma.

Article Snippet: In addition, Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide alone or with combination plus pan-caspase inhibitor Z-VAD-FMK (10 µM) or RIP kinase inhibitor necrostain 1 (10 µM) or 2 mM NAC (MedChem Express) for 48 h before being subjected to cell viability assay.

Techniques: Incubation, Staining, Flow Cytometry, Western Blot

Pimozide treatment increases ROS generation in HCC cells. (A) Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide, 2 mM NAC, or both for 48 h, and then stained with DCFH-DA. (A) Analysis of ROS production intensity among assayed cell populations. Representative images from one of three independent experiments are shown. (B) CCK-8 cell viability assay. Mean values from three independent experiments are shown with SDs, **P<0.01, compared to the control. ROS, reactive oxygen species; HCC, hepatocellular carcinoma; NAC, N-acetyl-l-cysteine.

Journal: Oncology Reports

Article Title: Antipsychotic agent pimozide promotes reversible proliferative suppression by inducing cellular quiescence in liver cancer

doi: 10.3892/or.2019.7229

Figure Lengend Snippet: Pimozide treatment increases ROS generation in HCC cells. (A) Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide, 2 mM NAC, or both for 48 h, and then stained with DCFH-DA. (A) Analysis of ROS production intensity among assayed cell populations. Representative images from one of three independent experiments are shown. (B) CCK-8 cell viability assay. Mean values from three independent experiments are shown with SDs, **P<0.01, compared to the control. ROS, reactive oxygen species; HCC, hepatocellular carcinoma; NAC, N-acetyl-l-cysteine.

Article Snippet: In addition, Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide alone or with combination plus pan-caspase inhibitor Z-VAD-FMK (10 µM) or RIP kinase inhibitor necrostain 1 (10 µM) or 2 mM NAC (MedChem Express) for 48 h before being subjected to cell viability assay.

Techniques: Staining, CCK-8 Assay, Viability Assay, Control

Pimozide enhances the proliferative inhibition of sorafenib in HCC cells. Sk-hep1 and Huh7 cells were treated with 5 µM pimozide, sorafenib, or both, and then subjected to cell viability (A) and colony formation (B) assays. Representative images from one of three independent experiments are shown. (C) Mean values from three independent experiments are shown with SDs. The data are summarized from 3 independent experiments, **P<0.01, compared to the control. HCC, hepatocellular carcinoma.

Journal: Oncology Reports

Article Title: Antipsychotic agent pimozide promotes reversible proliferative suppression by inducing cellular quiescence in liver cancer

doi: 10.3892/or.2019.7229

Figure Lengend Snippet: Pimozide enhances the proliferative inhibition of sorafenib in HCC cells. Sk-hep1 and Huh7 cells were treated with 5 µM pimozide, sorafenib, or both, and then subjected to cell viability (A) and colony formation (B) assays. Representative images from one of three independent experiments are shown. (C) Mean values from three independent experiments are shown with SDs. The data are summarized from 3 independent experiments, **P<0.01, compared to the control. HCC, hepatocellular carcinoma.

Article Snippet: In addition, Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide alone or with combination plus pan-caspase inhibitor Z-VAD-FMK (10 µM) or RIP kinase inhibitor necrostain 1 (10 µM) or 2 mM NAC (MedChem Express) for 48 h before being subjected to cell viability assay.

Techniques: Inhibition, Control