HY-124947 Search Results


ldc1267  (MedChemExpress)
93
MedChemExpress ldc1267
AXL and its kinase activity are required for GAS6-mediated internalization of GAS6–AXL complexes. A Confocal images showing GAS6–AXL internalization upon knockout of AXL and TYRO3 in LN229 cells. Two gRNAs targeting AXL (gAXL#1 and gAXL#2) and TYRO3 (gTYRO3#1 and TYRO3#2) were used. CRISPR-Cas9-edited LN229 cells with two non-targeting gRNAs (gNT#1 and gNT#2) served as controls. Serum-starved cells were stimulated with GAS6 for 5 min. B , C Quantification of number (B) and integral fluorescence intensity ( C ) of GAS6- and AXL-positive vesicles in CRISPR-Cas9-mediated knockouts of AXL and TYRO3 (representative confocal images shown in ( A ), n = 4. Student’s one-sample t test, *** p ≤ 0.001, **** p ≤ 0.0001, ns non-significant ( p > 0.05). D Western blot showing phosphorylation of AXL (P-AXL, Y702) after treatment of LN229 cells with AXL inhibitors. Serum-starved cells were pre-treated with R428 or <t>LDC1267,</t> or DMSO as a solvent control, and stimulated with GAS6 for 5 min. α-Tubulin served as a loading control. E Confocal images showing the internalization of GAS6–AXL complexes upon pharmacological inhibition of AXL. LN229 cells were treated as described in ( D ). F Quantification of number and integral fluorescence intensity of GAS6- and AXL-positive vesicles in LN229 cells after treatment with R428 and LDC1267 (representative confocal images shown in ( E ), n = 3. Student’s one-sample t test, * p ≤ 0,05, ** p ≤ 0.01, *** p ≤ 0.001, ns non-significant ( p > 0.05). Data information: Insets in confocal images show magnified views of boxed regions in the main images. Scale bars: 20 μm. For data quantification, approximately 150 cells were analyzed per experiment. Each dot represents data from one independent experiment, whereas bars represent the means ± SEM from n experiments. WT wild type LN229 cells, NS non-stimulated cells, GAS6 GAS6-stimulated cells
Ldc1267, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ldc1267/product/MedChemExpress
Average 93 stars, based on 1 article reviews
ldc1267 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


AXL and its kinase activity are required for GAS6-mediated internalization of GAS6–AXL complexes. A Confocal images showing GAS6–AXL internalization upon knockout of AXL and TYRO3 in LN229 cells. Two gRNAs targeting AXL (gAXL#1 and gAXL#2) and TYRO3 (gTYRO3#1 and TYRO3#2) were used. CRISPR-Cas9-edited LN229 cells with two non-targeting gRNAs (gNT#1 and gNT#2) served as controls. Serum-starved cells were stimulated with GAS6 for 5 min. B , C Quantification of number (B) and integral fluorescence intensity ( C ) of GAS6- and AXL-positive vesicles in CRISPR-Cas9-mediated knockouts of AXL and TYRO3 (representative confocal images shown in ( A ), n = 4. Student’s one-sample t test, *** p ≤ 0.001, **** p ≤ 0.0001, ns non-significant ( p > 0.05). D Western blot showing phosphorylation of AXL (P-AXL, Y702) after treatment of LN229 cells with AXL inhibitors. Serum-starved cells were pre-treated with R428 or LDC1267, or DMSO as a solvent control, and stimulated with GAS6 for 5 min. α-Tubulin served as a loading control. E Confocal images showing the internalization of GAS6–AXL complexes upon pharmacological inhibition of AXL. LN229 cells were treated as described in ( D ). F Quantification of number and integral fluorescence intensity of GAS6- and AXL-positive vesicles in LN229 cells after treatment with R428 and LDC1267 (representative confocal images shown in ( E ), n = 3. Student’s one-sample t test, * p ≤ 0,05, ** p ≤ 0.01, *** p ≤ 0.001, ns non-significant ( p > 0.05). Data information: Insets in confocal images show magnified views of boxed regions in the main images. Scale bars: 20 μm. For data quantification, approximately 150 cells were analyzed per experiment. Each dot represents data from one independent experiment, whereas bars represent the means ± SEM from n experiments. WT wild type LN229 cells, NS non-stimulated cells, GAS6 GAS6-stimulated cells

Journal: Cellular and Molecular Life Sciences

Article Title: Endocytic trafficking of GAS6–AXL complexes is associated with sustained AKT activation

doi: 10.1007/s00018-022-04312-3

Figure Lengend Snippet: AXL and its kinase activity are required for GAS6-mediated internalization of GAS6–AXL complexes. A Confocal images showing GAS6–AXL internalization upon knockout of AXL and TYRO3 in LN229 cells. Two gRNAs targeting AXL (gAXL#1 and gAXL#2) and TYRO3 (gTYRO3#1 and TYRO3#2) were used. CRISPR-Cas9-edited LN229 cells with two non-targeting gRNAs (gNT#1 and gNT#2) served as controls. Serum-starved cells were stimulated with GAS6 for 5 min. B , C Quantification of number (B) and integral fluorescence intensity ( C ) of GAS6- and AXL-positive vesicles in CRISPR-Cas9-mediated knockouts of AXL and TYRO3 (representative confocal images shown in ( A ), n = 4. Student’s one-sample t test, *** p ≤ 0.001, **** p ≤ 0.0001, ns non-significant ( p > 0.05). D Western blot showing phosphorylation of AXL (P-AXL, Y702) after treatment of LN229 cells with AXL inhibitors. Serum-starved cells were pre-treated with R428 or LDC1267, or DMSO as a solvent control, and stimulated with GAS6 for 5 min. α-Tubulin served as a loading control. E Confocal images showing the internalization of GAS6–AXL complexes upon pharmacological inhibition of AXL. LN229 cells were treated as described in ( D ). F Quantification of number and integral fluorescence intensity of GAS6- and AXL-positive vesicles in LN229 cells after treatment with R428 and LDC1267 (representative confocal images shown in ( E ), n = 3. Student’s one-sample t test, * p ≤ 0,05, ** p ≤ 0.01, *** p ≤ 0.001, ns non-significant ( p > 0.05). Data information: Insets in confocal images show magnified views of boxed regions in the main images. Scale bars: 20 μm. For data quantification, approximately 150 cells were analyzed per experiment. Each dot represents data from one independent experiment, whereas bars represent the means ± SEM from n experiments. WT wild type LN229 cells, NS non-stimulated cells, GAS6 GAS6-stimulated cells

Article Snippet: Cycloheximide (8682.1, used at 10 µg/mL) from Carl Roth GmbH, AXL inhibitors: R428 (HY-15150, used at 5 µM) and LDC1267 (HY-12494, used at 5 µM) from MedChemExpress.

Techniques: Activity Assay, Knock-Out, CRISPR, Fluorescence, Western Blot, Solvent, Control, Inhibition