HY-122128 Search Results


ml 60218  (MedChemExpress)
94
MedChemExpress ml 60218
Ml 60218, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ml 60218/product/MedChemExpress
Average 94 stars, based on 1 article reviews
ml 60218 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
omaveloxolone rta 408  (MedChemExpress)
94
MedChemExpress omaveloxolone rta 408
Pharmacological validation of Nrf2/ARE reporter fish. ( A ) Whole-mount bright field and fluorescence microscopy acquisition of a 24 hpf transgenic larva treated with DMSO and the Nrf2 pathway agonist, <t>RTA-408,</t> for 16 h. A significant increase in fluorescent cells is detected in RTA-408-treated larvae when compared to DMSO-treated fish. ( B ) Whole-mount bright field and fluorescence microscopy acquisition of a 24 hpf transgenic larva treated with DMSO and the Nrf2 pathway antagonist, Brusatol, for 16 h. A visible decrease in reporter fluorescent intensity is visible in Brusatol-treated larvae. All images are lateral views with anterior to the left. The magnifications of the trunk regions are confocal Z-stack acquisitions. The graphs reported on the right depict the ImageJ-based quantification of the selected trunk region of 5 independently treated fish (* p < 0.05; ** p < 0.005, t -test). Scale bars in the panels with multiple fish: 500 μm; scale bars on the panels with a single magnified larva: 100 μm.
Omaveloxolone Rta 408, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/omaveloxolone rta 408/product/MedChemExpress
Average 94 stars, based on 1 article reviews
omaveloxolone rta 408 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier

Image Search Results


Pharmacological validation of Nrf2/ARE reporter fish. ( A ) Whole-mount bright field and fluorescence microscopy acquisition of a 24 hpf transgenic larva treated with DMSO and the Nrf2 pathway agonist, RTA-408, for 16 h. A significant increase in fluorescent cells is detected in RTA-408-treated larvae when compared to DMSO-treated fish. ( B ) Whole-mount bright field and fluorescence microscopy acquisition of a 24 hpf transgenic larva treated with DMSO and the Nrf2 pathway antagonist, Brusatol, for 16 h. A visible decrease in reporter fluorescent intensity is visible in Brusatol-treated larvae. All images are lateral views with anterior to the left. The magnifications of the trunk regions are confocal Z-stack acquisitions. The graphs reported on the right depict the ImageJ-based quantification of the selected trunk region of 5 independently treated fish (* p < 0.05; ** p < 0.005, t -test). Scale bars in the panels with multiple fish: 500 μm; scale bars on the panels with a single magnified larva: 100 μm.

Journal: International Journal of Molecular Sciences

Article Title: Monitoring Nrf2/ARE Pathway Activity with a New Zebrafish Reporter System

doi: 10.3390/ijms24076804

Figure Lengend Snippet: Pharmacological validation of Nrf2/ARE reporter fish. ( A ) Whole-mount bright field and fluorescence microscopy acquisition of a 24 hpf transgenic larva treated with DMSO and the Nrf2 pathway agonist, RTA-408, for 16 h. A significant increase in fluorescent cells is detected in RTA-408-treated larvae when compared to DMSO-treated fish. ( B ) Whole-mount bright field and fluorescence microscopy acquisition of a 24 hpf transgenic larva treated with DMSO and the Nrf2 pathway antagonist, Brusatol, for 16 h. A visible decrease in reporter fluorescent intensity is visible in Brusatol-treated larvae. All images are lateral views with anterior to the left. The magnifications of the trunk regions are confocal Z-stack acquisitions. The graphs reported on the right depict the ImageJ-based quantification of the selected trunk region of 5 independently treated fish (* p < 0.05; ** p < 0.005, t -test). Scale bars in the panels with multiple fish: 500 μm; scale bars on the panels with a single magnified larva: 100 μm.

Article Snippet: For drug screening tests, 8 hpf or 24 hpf embryos were treated for 12 and 24 h with 5 μM ML-385, 500 nM Brusatol, 10 μM dimethyl fumarate (DMF), 2 μg/mL Edaravone (MCI-186) (Merck, Milan, Italy), 500 nM Omaveloxolone (RTA-408) (Medchemexpress, Dba, Milan, Italy) and 5 μM CHIR99021 (Merck, Milan, Italy).

Techniques: Biomarker Discovery, Fluorescence, Microscopy, Transgenic Assay