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aliskiren  (MedChemExpress)
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Role of S1P (site-1 protease)-derived sPRR (soluble [pro]renin receptor) in mediation of Ang II (angiotensin II)–induced ENaC activation in vitro. Confluent mouse cortical collecting duct cell line (mpkCCD) cells grown on Snapwells were pretreated with 10 μmol/L PF for 1 h and then treated with 1 μmol/L Ang II and 10 nmol/L sPRR-His for 24 h. Amiloride-sensitive transepithelial Na + transport, an index of ENaC activity, was recorded by the Ussing chamber technique. Medium sPRR was measured by using ELISA. A , ENaC activity in CTR, Ang II, Ang II + PF, and Ang II + PF + sPRR-His groups. Data are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II. $ P <0.05 vs Ang II + PF. B , Medium sPRR content in cells treated with CTR, Ang II, or Ang II + PF were determined by using ELISA. Data are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II. C , The cells were transfected with S1P siRNA or scrambled siRNA, followed by 24-h Ang II treatment and Ussing chamber measurement of ENaC activity. Date are mean ± SE. N=4 per group. * P <0.05 vs scrambled siRNA. D , The cells were pretreated with 1 μmol/L <t>Aliskiren</t> for 0.5 h or 10 μmol/L PF for 1 h, followed by 24-h Ang II treatment and Ussing chamber measurement of ENaC activity. Date are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II.
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Role of S1P (site-1 protease)-derived sPRR (soluble [pro]renin receptor) in mediation of Ang II (angiotensin II)–induced ENaC activation in vitro. Confluent mouse cortical collecting duct cell line (mpkCCD) cells grown on Snapwells were pretreated with 10 μmol/L PF for 1 h and then treated with 1 μmol/L Ang II and 10 nmol/L sPRR-His for 24 h. Amiloride-sensitive transepithelial Na + transport, an index of ENaC activity, was recorded by the Ussing chamber technique. Medium sPRR was measured by using ELISA. A , ENaC activity in CTR, Ang II, Ang II + PF, and Ang II + PF + sPRR-His groups. Data are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II. $ P <0.05 vs Ang II + PF. B , Medium sPRR content in cells treated with CTR, Ang II, or Ang II + PF were determined by using ELISA. Data are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II. C , The cells were transfected with S1P siRNA or scrambled siRNA, followed by 24-h Ang II treatment and Ussing chamber measurement of ENaC activity. Date are mean ± SE. N=4 per group. * P <0.05 vs scrambled siRNA. D , The cells were pretreated with 1 μmol/L Aliskiren for 0.5 h or 10 μmol/L PF for 1 h, followed by 24-h Ang II treatment and Ussing chamber measurement of ENaC activity. Date are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II.

Journal: Hypertension (Dallas, Tex. : 1979)

Article Title: Site-1 Protease-Derived Soluble (Pro)Renin Receptor Contributes to Angiotensin II–Induced Hypertension in Mice

doi: 10.1161/HYPERTENSIONAHA.120.15100

Figure Lengend Snippet: Role of S1P (site-1 protease)-derived sPRR (soluble [pro]renin receptor) in mediation of Ang II (angiotensin II)–induced ENaC activation in vitro. Confluent mouse cortical collecting duct cell line (mpkCCD) cells grown on Snapwells were pretreated with 10 μmol/L PF for 1 h and then treated with 1 μmol/L Ang II and 10 nmol/L sPRR-His for 24 h. Amiloride-sensitive transepithelial Na + transport, an index of ENaC activity, was recorded by the Ussing chamber technique. Medium sPRR was measured by using ELISA. A , ENaC activity in CTR, Ang II, Ang II + PF, and Ang II + PF + sPRR-His groups. Data are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II. $ P <0.05 vs Ang II + PF. B , Medium sPRR content in cells treated with CTR, Ang II, or Ang II + PF were determined by using ELISA. Data are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II. C , The cells were transfected with S1P siRNA or scrambled siRNA, followed by 24-h Ang II treatment and Ussing chamber measurement of ENaC activity. Date are mean ± SE. N=4 per group. * P <0.05 vs scrambled siRNA. D , The cells were pretreated with 1 μmol/L Aliskiren for 0.5 h or 10 μmol/L PF for 1 h, followed by 24-h Ang II treatment and Ussing chamber measurement of ENaC activity. Date are mean ± SE. N=4 per group. * P <0.05 vs CTR. # P <0.05 vs Ang II.

Article Snippet: The cells were pretreated with Aliskiren (1 μmol/L; catalog no. HY-12176, MCE) for 0.5 hours or PF (10 μmol/L) for 1 hour or transfected with S1P siRNA or the corresponding scrambled siRNA for 48 hours, and then treated with Ang II (1 μmol/L) or sPRR-His (10 nmol/L) for 24 hours.

Techniques: Derivative Assay, Activation Assay, In Vitro, Activity Assay, Enzyme-linked Immunosorbent Assay, Transfection