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gabbr2 agonist cgp36742  (MedChemExpress)
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MedChemExpress gabbr2 agonist cgp36742
a) Venn diagrams shows the epigenetically upregulated genes newly identified in GIC classified in subgroups defined by a characteristic of interest for further molecular validation or clinical application. b) Venn diagrams shows the epigenetically downregulated genes newly identified in GIC classified in subgroups defined by a characteristic of interest for further molecular validation or clinical application. c) Representative images of SMOX protein expression (green) in iNSC and GIC from patient 52. Nuclei are counterstained with DAPI. Scale bar:100µm. Quantification of results is shown as Mean Fluorescence Intensity (MFI) standardised by the number of nuclei for three patients. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001, **** p value<0.0001. d) Representative bright-field images show wound healing assay in GIC19 cells treated for 24hours with 10µM of SMOX inhibitor MD72527. Quantification of results is expressed in wound area at 24 hours standardised on t0 for GIC from two patients. Experiments have been performed three times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. e) Invasion assay quantification of results expressed in fold change of the average number of nuclei per image field of GIC from patients 19 and 61 treated with 10µM of SMOX inhibitor MDL72527 standardised on the vehicle. Experiments have been performed three times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. f) Representative bright-field images show wound healing assay in GIC19 cells treated for 24 hours with 100µM of <t>GABBR2</t> agonist Baclofen or 200 µM of GABBR2 antagonist <t>CGP36742.</t> Quantification of results is expressed in wound area at 24 hours standardised on t0 for GIC from two patients. Experiments have been repeated three independent times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. g) Invasion assay quantification of results expressed in fold change of the average number of nuclei per image field of GIC from patients 19 and 61 treated with 100µM of GABBR2 agonist Baclofen or 200 µM of GABBR2 antagonist CGP36742 standardised on the vehicle. Experiments have been repeated three independent times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001.
Gabbr2 Agonist Cgp36742, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a) Venn diagrams shows the epigenetically upregulated genes newly identified in GIC classified in subgroups defined by a characteristic of interest for further molecular validation or clinical application. b) Venn diagrams shows the epigenetically downregulated genes newly identified in GIC classified in subgroups defined by a characteristic of interest for further molecular validation or clinical application. c) Representative images of SMOX protein expression (green) in iNSC and GIC from patient 52. Nuclei are counterstained with DAPI. Scale bar:100µm. Quantification of results is shown as Mean Fluorescence Intensity (MFI) standardised by the number of nuclei for three patients. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001, **** p value<0.0001. d) Representative bright-field images show wound healing assay in GIC19 cells treated for 24hours with 10µM of SMOX inhibitor MD72527. Quantification of results is expressed in wound area at 24 hours standardised on t0 for GIC from two patients. Experiments have been performed three times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. e) Invasion assay quantification of results expressed in fold change of the average number of nuclei per image field of GIC from patients 19 and 61 treated with 10µM of SMOX inhibitor MDL72527 standardised on the vehicle. Experiments have been performed three times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. f) Representative bright-field images show wound healing assay in GIC19 cells treated for 24 hours with 100µM of GABBR2 agonist Baclofen or 200 µM of GABBR2 antagonist CGP36742. Quantification of results is expressed in wound area at 24 hours standardised on t0 for GIC from two patients. Experiments have been repeated three independent times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. g) Invasion assay quantification of results expressed in fold change of the average number of nuclei per image field of GIC from patients 19 and 61 treated with 100µM of GABBR2 agonist Baclofen or 200 µM of GABBR2 antagonist CGP36742 standardised on the vehicle. Experiments have been repeated three independent times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001.

Journal: bioRxiv

Article Title: Mapping chromatin remodelling in glioblastoma identifies epigenetic regulation of key molecular pathways and novel druggable targets

doi: 10.1101/2024.02.24.581853

Figure Lengend Snippet: a) Venn diagrams shows the epigenetically upregulated genes newly identified in GIC classified in subgroups defined by a characteristic of interest for further molecular validation or clinical application. b) Venn diagrams shows the epigenetically downregulated genes newly identified in GIC classified in subgroups defined by a characteristic of interest for further molecular validation or clinical application. c) Representative images of SMOX protein expression (green) in iNSC and GIC from patient 52. Nuclei are counterstained with DAPI. Scale bar:100µm. Quantification of results is shown as Mean Fluorescence Intensity (MFI) standardised by the number of nuclei for three patients. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001, **** p value<0.0001. d) Representative bright-field images show wound healing assay in GIC19 cells treated for 24hours with 10µM of SMOX inhibitor MD72527. Quantification of results is expressed in wound area at 24 hours standardised on t0 for GIC from two patients. Experiments have been performed three times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. e) Invasion assay quantification of results expressed in fold change of the average number of nuclei per image field of GIC from patients 19 and 61 treated with 10µM of SMOX inhibitor MDL72527 standardised on the vehicle. Experiments have been performed three times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. f) Representative bright-field images show wound healing assay in GIC19 cells treated for 24 hours with 100µM of GABBR2 agonist Baclofen or 200 µM of GABBR2 antagonist CGP36742. Quantification of results is expressed in wound area at 24 hours standardised on t0 for GIC from two patients. Experiments have been repeated three independent times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001. g) Invasion assay quantification of results expressed in fold change of the average number of nuclei per image field of GIC from patients 19 and 61 treated with 100µM of GABBR2 agonist Baclofen or 200 µM of GABBR2 antagonist CGP36742 standardised on the vehicle. Experiments have been repeated three independent times. One-way ANOVA test. * p value<0.05, ** p value<0.01, *** p value<0.001.

Article Snippet: A three days treatment was performed with increasing doses of GABBR2 agonist CGP36742 (MedChem Express HY-121599 at 100 and 200μM), GABBR2 antagonist Baclofen (Tocris, 0417 at 10 and 100 μM) and SMOX inhibitor MDL72527 (Sigma, M2949 at 10 μM) or vehicle.

Techniques: Expressing, Fluorescence, Wound Healing Assay, Invasion Assay