HY-117938 Search Results


danicopan  (MedChemExpress)
94
MedChemExpress danicopan
Danicopan, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/danicopan/product/MedChemExpress
Average 94 stars, based on 1 article reviews
danicopan - by Bioz Stars, 2026-02
94/100 stars
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mrt67307  (MedChemExpress)
94
MedChemExpress mrt67307
a, b HepG2/GFP-Gal3 cells were treated with either <t>MRT67307</t> (2 µM) or GSK8612 (10 µM) in combination with LLOMe (750 µM). After the 2-h treatment, LLOMe was washed out with normal cell culture media, and the cells were further incubated with either MRT67307 or GSK8612 for 24 h. The number of GFP-Gal3 puncta was quantified, and the cells were further analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing an individual HepG2 cell ( n = 300, * p < 0.0001). Scale bar: 10 µm. c HepG2 cells transiently expressing both GFP-TBK1 and HA-FBXO3 were treated with LLOMe. Subsequently, the cells were subjected to immunoprecipitation using an anti-HA antibody conjugated with agarose beads. The immunoprecipitates were further analyzed by western blotting using the indicated antibodies. Data in all panels are presented as the mean ( n = 2). d HepG2 cells were treated with LLOMe and subsequently lysed. The cell lysates were subjected to immunoprecipitation using an anti-FBXO3 antibody. Then, the immunoprecipitates were analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing independent triplicate replicates of the experiment ( n = 3, * p = 0.0212). e HepG2 cells overexpressing either HA or HA-FBXO3 were pretreated with MRT67307 (2 µM) for 2 h, followed by cotreatment with LLOMe. The cells were then subjected to analysis by Phos-tag™ SDS-PAGE and western blotting using the indicated antibodies. All experiments were replicated three times except c (twice replicates). Two-tailed unpaired t -test were used to analyzed all the data. Source data are provided as a Source Data file.
Mrt67307, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mrt67307/product/MedChemExpress
Average 94 stars, based on 1 article reviews
mrt67307 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier

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a, b HepG2/GFP-Gal3 cells were treated with either MRT67307 (2 µM) or GSK8612 (10 µM) in combination with LLOMe (750 µM). After the 2-h treatment, LLOMe was washed out with normal cell culture media, and the cells were further incubated with either MRT67307 or GSK8612 for 24 h. The number of GFP-Gal3 puncta was quantified, and the cells were further analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing an individual HepG2 cell ( n = 300, * p < 0.0001). Scale bar: 10 µm. c HepG2 cells transiently expressing both GFP-TBK1 and HA-FBXO3 were treated with LLOMe. Subsequently, the cells were subjected to immunoprecipitation using an anti-HA antibody conjugated with agarose beads. The immunoprecipitates were further analyzed by western blotting using the indicated antibodies. Data in all panels are presented as the mean ( n = 2). d HepG2 cells were treated with LLOMe and subsequently lysed. The cell lysates were subjected to immunoprecipitation using an anti-FBXO3 antibody. Then, the immunoprecipitates were analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing independent triplicate replicates of the experiment ( n = 3, * p = 0.0212). e HepG2 cells overexpressing either HA or HA-FBXO3 were pretreated with MRT67307 (2 µM) for 2 h, followed by cotreatment with LLOMe. The cells were then subjected to analysis by Phos-tag™ SDS-PAGE and western blotting using the indicated antibodies. All experiments were replicated three times except c (twice replicates). Two-tailed unpaired t -test were used to analyzed all the data. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Activation of lysophagy by a TBK1-SCF FBXO3 -TMEM192-TAX1BP1 axis in response to lysosomal damage

doi: 10.1038/s41467-025-56294-y

Figure Lengend Snippet: a, b HepG2/GFP-Gal3 cells were treated with either MRT67307 (2 µM) or GSK8612 (10 µM) in combination with LLOMe (750 µM). After the 2-h treatment, LLOMe was washed out with normal cell culture media, and the cells were further incubated with either MRT67307 or GSK8612 for 24 h. The number of GFP-Gal3 puncta was quantified, and the cells were further analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing an individual HepG2 cell ( n = 300, * p < 0.0001). Scale bar: 10 µm. c HepG2 cells transiently expressing both GFP-TBK1 and HA-FBXO3 were treated with LLOMe. Subsequently, the cells were subjected to immunoprecipitation using an anti-HA antibody conjugated with agarose beads. The immunoprecipitates were further analyzed by western blotting using the indicated antibodies. Data in all panels are presented as the mean ( n = 2). d HepG2 cells were treated with LLOMe and subsequently lysed. The cell lysates were subjected to immunoprecipitation using an anti-FBXO3 antibody. Then, the immunoprecipitates were analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing independent triplicate replicates of the experiment ( n = 3, * p = 0.0212). e HepG2 cells overexpressing either HA or HA-FBXO3 were pretreated with MRT67307 (2 µM) for 2 h, followed by cotreatment with LLOMe. The cells were then subjected to analysis by Phos-tag™ SDS-PAGE and western blotting using the indicated antibodies. All experiments were replicated three times except c (twice replicates). Two-tailed unpaired t -test were used to analyzed all the data. Source data are provided as a Source Data file.

Article Snippet: BC-1215 (HY-117937), GSK8612 (HY-111941) and MRT67307 (HY-13018) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Cell Culture, Incubation, Western Blot, Expressing, Immunoprecipitation, SDS Page, Two Tailed Test

a HepG2 cells were transfected with both HA-ubiquitin and TMEM192-FLAG and pretreated with either MRT67307 (2 µM) or GSK8612 (10 µM). LLOMe was cotreated for an additional 2 h. Subsequently, the cells were immunoprecipitated with agarose bead-conjugated anti-FLAG antibody and further analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing independent triplicate replicates of the experiment ( n = 3, * p = 0.0446, ** p = 0.0257, *** p = 0.0088). b HepG2 cells overexpressing both GFP-TMEM192 and TAX1BP1-FLAG were pretreated with either BC-1215 or GSK8612. LLOMe was cotreated for an additional 2 h. The cells were then pulled down with an anti-GFP antibody conjugated with agarose beads and further analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing independent triplicate replicates of the experiment ( n = 3, * p = 0.0171, ** p = 0.0006, *** p = 0.0002). c HepG2 cells transiently expressing GFP-LC3 and TMEM192-FLAG were pretreated with either BC-1215 or GSK8612 and cotreated with LLOMe. Subsequently, the cells were stained with an anti-FLAG antibody and imaged by confocal microscopy. The colocalization extent of TMEM192 with LC3 was analyzed using Pearson’s correlation coefficients. Data are presented as the mean ± SEM, with each dot in the plot representing an individual HepG2 cell ( n = 15, * p = 0.0031, ** p < 0.0001). Scale bar: 10 µm. All experiments were replicated three times. Two-tailed unpaired t -test were used to analyzed all the data. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Activation of lysophagy by a TBK1-SCF FBXO3 -TMEM192-TAX1BP1 axis in response to lysosomal damage

doi: 10.1038/s41467-025-56294-y

Figure Lengend Snippet: a HepG2 cells were transfected with both HA-ubiquitin and TMEM192-FLAG and pretreated with either MRT67307 (2 µM) or GSK8612 (10 µM). LLOMe was cotreated for an additional 2 h. Subsequently, the cells were immunoprecipitated with agarose bead-conjugated anti-FLAG antibody and further analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing independent triplicate replicates of the experiment ( n = 3, * p = 0.0446, ** p = 0.0257, *** p = 0.0088). b HepG2 cells overexpressing both GFP-TMEM192 and TAX1BP1-FLAG were pretreated with either BC-1215 or GSK8612. LLOMe was cotreated for an additional 2 h. The cells were then pulled down with an anti-GFP antibody conjugated with agarose beads and further analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM, with each dot in the plot representing independent triplicate replicates of the experiment ( n = 3, * p = 0.0171, ** p = 0.0006, *** p = 0.0002). c HepG2 cells transiently expressing GFP-LC3 and TMEM192-FLAG were pretreated with either BC-1215 or GSK8612 and cotreated with LLOMe. Subsequently, the cells were stained with an anti-FLAG antibody and imaged by confocal microscopy. The colocalization extent of TMEM192 with LC3 was analyzed using Pearson’s correlation coefficients. Data are presented as the mean ± SEM, with each dot in the plot representing an individual HepG2 cell ( n = 15, * p = 0.0031, ** p < 0.0001). Scale bar: 10 µm. All experiments were replicated three times. Two-tailed unpaired t -test were used to analyzed all the data. Source data are provided as a Source Data file.

Article Snippet: BC-1215 (HY-117937), GSK8612 (HY-111941) and MRT67307 (HY-13018) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Transfection, Immunoprecipitation, Western Blot, Expressing, Staining, Confocal Microscopy, Two Tailed Test