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ddd107498  (MedChemExpress)
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MedChemExpress ddd107498
Translation inhibition was quantified in P. berghei EEFs (A-B) and matching in-image HepG2 (A) following acute pre-treatment from 24 - 28 hpi, as shown in the schematic. Compounds were tested in 8 or 10 point, 3-fold serial dilution. (A) Concentration response curves. Single data points represent the mean translation at each concentration, normalized to DMSO controls, and errors bars show standard deviation from n=3 independent experiments. (B) Single LS parasite concentration response. For each compound, concentrations plotted moving from lowest (left) to highest (right) as indicated by the triangles. Top concentrations tested were 3,333 nM for anisomycin and <t>DDD107498,</t> 123 nM for bruceantin, 16,667 nM for MMV01266, and 100,000 nM for LysRS-IN-2. N=3 independent experiments combined, each dot represents mean translation intensity (OPP-MFI) in a single parasite normalized to DMSO controls, n=10,461EEFs total. The full concentration-response dataset can be explored via interactive dashboards in in our KNIME hub workflow: https://hub.knime.com/-/spaces/-/~TZCrKvv3sbJwM_xP/current-state/ . (C) Experimental setup to probe the effects of acute translation inhibition and the relationship between translation inhibition efficacy and antiplasmodial efficacy with inhibitors tested at equivalent effective concentrations.
Ddd107498, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ddd107498 - by Bioz Stars, 2026-02
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Translation inhibition was quantified in P. berghei EEFs (A-B) and matching in-image HepG2 (A) following acute pre-treatment from 24 - 28 hpi, as shown in the schematic. Compounds were tested in 8 or 10 point, 3-fold serial dilution. (A) Concentration response curves. Single data points represent the mean translation at each concentration, normalized to DMSO controls, and errors bars show standard deviation from n=3 independent experiments. (B) Single LS parasite concentration response. For each compound, concentrations plotted moving from lowest (left) to highest (right) as indicated by the triangles. Top concentrations tested were 3,333 nM for anisomycin and DDD107498, 123 nM for bruceantin, 16,667 nM for MMV01266, and 100,000 nM for LysRS-IN-2. N=3 independent experiments combined, each dot represents mean translation intensity (OPP-MFI) in a single parasite normalized to DMSO controls, n=10,461EEFs total. The full concentration-response dataset can be explored via interactive dashboards in in our KNIME hub workflow: https://hub.knime.com/-/spaces/-/~TZCrKvv3sbJwM_xP/current-state/ . (C) Experimental setup to probe the effects of acute translation inhibition and the relationship between translation inhibition efficacy and antiplasmodial efficacy with inhibitors tested at equivalent effective concentrations.

Journal: bioRxiv

Article Title: Translation inhibition efficacy does not determine the Plasmodium berghei liver stage antiplasmodial efficacy of protein synthesis inhibitors

doi: 10.1101/2023.12.07.570699

Figure Lengend Snippet: Translation inhibition was quantified in P. berghei EEFs (A-B) and matching in-image HepG2 (A) following acute pre-treatment from 24 - 28 hpi, as shown in the schematic. Compounds were tested in 8 or 10 point, 3-fold serial dilution. (A) Concentration response curves. Single data points represent the mean translation at each concentration, normalized to DMSO controls, and errors bars show standard deviation from n=3 independent experiments. (B) Single LS parasite concentration response. For each compound, concentrations plotted moving from lowest (left) to highest (right) as indicated by the triangles. Top concentrations tested were 3,333 nM for anisomycin and DDD107498, 123 nM for bruceantin, 16,667 nM for MMV01266, and 100,000 nM for LysRS-IN-2. N=3 independent experiments combined, each dot represents mean translation intensity (OPP-MFI) in a single parasite normalized to DMSO controls, n=10,461EEFs total. The full concentration-response dataset can be explored via interactive dashboards in in our KNIME hub workflow: https://hub.knime.com/-/spaces/-/~TZCrKvv3sbJwM_xP/current-state/ . (C) Experimental setup to probe the effects of acute translation inhibition and the relationship between translation inhibition efficacy and antiplasmodial efficacy with inhibitors tested at equivalent effective concentrations.

Article Snippet: Anisomycin (EMD Millipore / Sigma, 176880), bruceantin (MedChem Express, HY-N0840), DDD107498 (Apex Bio, A8711), Lys-RS-IN2 (MedChem Express, Y-126130), and MMV019266 (Vitas-M Laboratory, STK845176) were solubilized in DMSO (Sigma-Aldrich D2650), aliquoted, and stored at −20°C.

Techniques: Inhibition, Serial Dilution, Concentration Assay, Standard Deviation

Antiplasmodial effects of DDD107498 on translationally-arrested P. berghei LS parasites. Legend on following page. . Antiplasmodial effects of DDD107498 on translationally-arrested P. berghei LS parasites. A) Experimental schematic for panels B-C quantifying translation recovery and growth at 48 hpi after DDD107498 treatment of anisomycin-arrested parasites in early schizogony. B) Representative single confocal images of translation in P. berghei LSs at 48 hpi. Merged images are pseudocolored as indicated with parasite (EEF) immunolabeled with α-HSP70, OPP-A555 labeling the nascent proteome, and DNA stained with Hoechst. Scale bar = 5 μm. C) Single parasite translation and size quantified at 48 hpi. Single points show individual EEFs color coded by independent experiment, with experimental means represented by large circles (n=2), and bars represent mean and standard deviation of the experiments. D) Experimental schematic for quantifying merosome release in experiment-matched wells at 72, 96, and 120 hpi, after DDD107498 treatment of translationally arrested early LS schizonts and subsequent washout. Stacked bar charts (E,H) report total merosomes collected at per timepoint from all experiments. F,I) The percent of total detached cells/ merosomes released after 72 hpi (% delayed) are reported as means with error bars showing standard deviation. G,J) Total detached cell/ merosome release normalized to the DMSO controls, with error bars showing standard deviation. Data shown from n=3 (E-G) or n=2 (H-J) independent experiments; DDD498 = DDD107498 in the axis labels for (E-J).

Journal: bioRxiv

Article Title: Translation inhibition efficacy does not determine the Plasmodium berghei liver stage antiplasmodial efficacy of protein synthesis inhibitors

doi: 10.1101/2023.12.07.570699

Figure Lengend Snippet: Antiplasmodial effects of DDD107498 on translationally-arrested P. berghei LS parasites. Legend on following page. . Antiplasmodial effects of DDD107498 on translationally-arrested P. berghei LS parasites. A) Experimental schematic for panels B-C quantifying translation recovery and growth at 48 hpi after DDD107498 treatment of anisomycin-arrested parasites in early schizogony. B) Representative single confocal images of translation in P. berghei LSs at 48 hpi. Merged images are pseudocolored as indicated with parasite (EEF) immunolabeled with α-HSP70, OPP-A555 labeling the nascent proteome, and DNA stained with Hoechst. Scale bar = 5 μm. C) Single parasite translation and size quantified at 48 hpi. Single points show individual EEFs color coded by independent experiment, with experimental means represented by large circles (n=2), and bars represent mean and standard deviation of the experiments. D) Experimental schematic for quantifying merosome release in experiment-matched wells at 72, 96, and 120 hpi, after DDD107498 treatment of translationally arrested early LS schizonts and subsequent washout. Stacked bar charts (E,H) report total merosomes collected at per timepoint from all experiments. F,I) The percent of total detached cells/ merosomes released after 72 hpi (% delayed) are reported as means with error bars showing standard deviation. G,J) Total detached cell/ merosome release normalized to the DMSO controls, with error bars showing standard deviation. Data shown from n=3 (E-G) or n=2 (H-J) independent experiments; DDD498 = DDD107498 in the axis labels for (E-J).

Article Snippet: Anisomycin (EMD Millipore / Sigma, 176880), bruceantin (MedChem Express, HY-N0840), DDD107498 (Apex Bio, A8711), Lys-RS-IN2 (MedChem Express, Y-126130), and MMV019266 (Vitas-M Laboratory, STK845176) were solubilized in DMSO (Sigma-Aldrich D2650), aliquoted, and stored at −20°C.

Techniques: Immunolabeling, Labeling, Staining, Standard Deviation