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trfs  (MedChemExpress)
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MedChemExpress trfs
Trfs, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mc1r agonist bms 470539  (MedChemExpress)
93
MedChemExpress mc1r agonist bms 470539
The primer sequences used in this study
Mc1r Agonist Bms 470539, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The primer sequences used in this study

Journal: The Journal of Headache and Pain

Article Title: LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion

doi: 10.1186/s10194-024-01733-2

Figure Lengend Snippet: The primer sequences used in this study

Article Snippet: The MC1R agonist BMS-470539 (BMS, 100 nM, MedChemExpress, China) was used, and the extracellular solution without BMS was defined as control [ ].

Techniques:

The antibodies used in this study

Journal: The Journal of Headache and Pain

Article Title: LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion

doi: 10.1186/s10194-024-01733-2

Figure Lengend Snippet: The antibodies used in this study

Article Snippet: The MC1R agonist BMS-470539 (BMS, 100 nM, MedChemExpress, China) was used, and the extracellular solution without BMS was defined as control [ ].

Techniques:

DHX30 protein interacted with Mc1r mRNA. a Venn diagram of the RNAs from the RNA-protein interaction online algorithm CatRAPID and pain-related genes. b RIP was used to validate the interaction between DHX30 and the overlapped RNAs. * P < 0.05, n = 3/group. c RNA-FISH and IF staining revealed the co-localization of DHX30 protein and Mc1r mRNA in the cytoplasm of TGNs. Scare bar = 50 μm. d RNA pull-down assay assessed Mc1r mRNA interacted with DHX30, the antisense served as control. * P < 0.05, n = 3/group

Journal: The Journal of Headache and Pain

Article Title: LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion

doi: 10.1186/s10194-024-01733-2

Figure Lengend Snippet: DHX30 protein interacted with Mc1r mRNA. a Venn diagram of the RNAs from the RNA-protein interaction online algorithm CatRAPID and pain-related genes. b RIP was used to validate the interaction between DHX30 and the overlapped RNAs. * P < 0.05, n = 3/group. c RNA-FISH and IF staining revealed the co-localization of DHX30 protein and Mc1r mRNA in the cytoplasm of TGNs. Scare bar = 50 μm. d RNA pull-down assay assessed Mc1r mRNA interacted with DHX30, the antisense served as control. * P < 0.05, n = 3/group

Article Snippet: The MC1R agonist BMS-470539 (BMS, 100 nM, MedChemExpress, China) was used, and the extracellular solution without BMS was defined as control [ ].

Techniques: Staining, Pull Down Assay, Control

Anxa10-203 expression was associated with MC1R. a Mc1r expression in the TG on 1, 3, 7, and 14 days after CCI-ION. *** P < 0.001, n = 4/group. b Correlation analysis between Anxa10-203 and Mc1r in the TG by Pearson correlation analysis. R 2 = 0.7023, P < 0.0001, n = 20. c Anxa10-203 knockdown reversed the Mc1r mRNA increase induced by CCI-ION. * P < 0.05, ** P < 0.01, n = 4/group. d , e Anxa10-203 knockdown reversed the MC1R protein increase induced by CCI-ION. * P < 0.05, n = 4/group. f Anxa10-203 was increased 7 days after LV-Anxa10-203 microinjection in naive mice. * P < 0.05, n = 3/group. g Mc1r mRNA was significantly increased in TG induced by Anxa10-203 over-expression. * P < 0.05, ** P < 0.01, n = 3/group. h - i Anxa10-203 over-expression induced increased MC1R protein in naive mice. * P < 0.05, n = 3/group

Journal: The Journal of Headache and Pain

Article Title: LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion

doi: 10.1186/s10194-024-01733-2

Figure Lengend Snippet: Anxa10-203 expression was associated with MC1R. a Mc1r expression in the TG on 1, 3, 7, and 14 days after CCI-ION. *** P < 0.001, n = 4/group. b Correlation analysis between Anxa10-203 and Mc1r in the TG by Pearson correlation analysis. R 2 = 0.7023, P < 0.0001, n = 20. c Anxa10-203 knockdown reversed the Mc1r mRNA increase induced by CCI-ION. * P < 0.05, ** P < 0.01, n = 4/group. d , e Anxa10-203 knockdown reversed the MC1R protein increase induced by CCI-ION. * P < 0.05, n = 4/group. f Anxa10-203 was increased 7 days after LV-Anxa10-203 microinjection in naive mice. * P < 0.05, n = 3/group. g Mc1r mRNA was significantly increased in TG induced by Anxa10-203 over-expression. * P < 0.05, ** P < 0.01, n = 3/group. h - i Anxa10-203 over-expression induced increased MC1R protein in naive mice. * P < 0.05, n = 3/group

Article Snippet: The MC1R agonist BMS-470539 (BMS, 100 nM, MedChemExpress, China) was used, and the extracellular solution without BMS was defined as control [ ].

Techniques: Expressing, Knockdown, Microinjection, Over Expression

Anxa10-203 over-expression enhanced Mc1r mRNA stability through DHX30. a , b RNA and protein expression of DHX30 in Sham and CCI mice with or without DHX30 silenced. * P < 0.05, ** P < 0.01, *** P <0.001, n = 4/group. c , d Expression of Mc1r in Sham and CCI-ION mice with or without DHX30 silenced was assessed by RT-qPCR ( c ) and WB ( d ). * P < 0.05, ** P < 0.01, n = 3/group for RT-qPCR, n =4/group for WB. e , f The expression of DHX30 mRNA ( e ) and protein ( f ) were evaluated in the TGNs with DHX30 knockdown and Anxa10-203 over-expression in vitro . * P < 0.05, ** P < 0.01, n = 4/group. g , h The expression of Mc1r mRNA ( g ) and protein ( h ) was evaluated in TGNs with DHX30 silenced and Anxa10-203 over-expressed in vitro . * P < 0.05, ** P < 0.01, *** P < 0.001, n = 4/group for RT-qPCR, n =3/group for WB. i Representative images of nascent protein in TGNs of the two groups. Scare bar = 20 μm. j Quantitative analysis of fluorescence intensity of nascent protein in the two groups. n = 10/group. k The decay of Mc1r mRNA in different groups. * P < 0.05, *** P < 0.001, n = 4/group. oe: over-expression, n.s: no significance

Journal: The Journal of Headache and Pain

Article Title: LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion

doi: 10.1186/s10194-024-01733-2

Figure Lengend Snippet: Anxa10-203 over-expression enhanced Mc1r mRNA stability through DHX30. a , b RNA and protein expression of DHX30 in Sham and CCI mice with or without DHX30 silenced. * P < 0.05, ** P < 0.01, *** P <0.001, n = 4/group. c , d Expression of Mc1r in Sham and CCI-ION mice with or without DHX30 silenced was assessed by RT-qPCR ( c ) and WB ( d ). * P < 0.05, ** P < 0.01, n = 3/group for RT-qPCR, n =4/group for WB. e , f The expression of DHX30 mRNA ( e ) and protein ( f ) were evaluated in the TGNs with DHX30 knockdown and Anxa10-203 over-expression in vitro . * P < 0.05, ** P < 0.01, n = 4/group. g , h The expression of Mc1r mRNA ( g ) and protein ( h ) was evaluated in TGNs with DHX30 silenced and Anxa10-203 over-expressed in vitro . * P < 0.05, ** P < 0.01, *** P < 0.001, n = 4/group for RT-qPCR, n =3/group for WB. i Representative images of nascent protein in TGNs of the two groups. Scare bar = 20 μm. j Quantitative analysis of fluorescence intensity of nascent protein in the two groups. n = 10/group. k The decay of Mc1r mRNA in different groups. * P < 0.05, *** P < 0.001, n = 4/group. oe: over-expression, n.s: no significance

Article Snippet: The MC1R agonist BMS-470539 (BMS, 100 nM, MedChemExpress, China) was used, and the extracellular solution without BMS was defined as control [ ].

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Knockdown, In Vitro, Fluorescence