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12z cells  (MedChemExpress)
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MedChemExpress 12z cells
Role of macrophage polarization on <t>12Z</t> cells. To investigate the impact of macrophages on endometriosis cells, we co‐cultured macrophages from different groups with 12Z cells using cell chambers. (A) CCK‐8 was used to assess the growth viability of 12Z cells. (B) Scratch assay was performed to detect the migration capacity of 12Z cells (scale bar: 200 μm). (C) A Transwell invasion assay was used to analyze the invasion capacity of 12Z cells (scale bar: 200 μm). Data represent mean ± SD from three independent experiments. ***: P < 0.001, compared with control exosomes; ## : P < 0.01, ### : P < 0.001, compared with EMs exosomes.
12z Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Role of macrophage polarization on 12Z cells. To investigate the impact of macrophages on endometriosis cells, we co‐cultured macrophages from different groups with 12Z cells using cell chambers. (A) CCK‐8 was used to assess the growth viability of 12Z cells. (B) Scratch assay was performed to detect the migration capacity of 12Z cells (scale bar: 200 μm). (C) A Transwell invasion assay was used to analyze the invasion capacity of 12Z cells (scale bar: 200 μm). Data represent mean ± SD from three independent experiments. ***: P < 0.001, compared with control exosomes; ## : P < 0.01, ### : P < 0.001, compared with EMs exosomes.

Journal: Journal of Cell Communication and Signaling

Article Title: Endometriosis‐derived exosomes encapsulated miR‐196a‐5p mediate macrophage polarization through regulation of the Hippo pathway

doi: 10.1002/ccs3.70020

Figure Lengend Snippet: Role of macrophage polarization on 12Z cells. To investigate the impact of macrophages on endometriosis cells, we co‐cultured macrophages from different groups with 12Z cells using cell chambers. (A) CCK‐8 was used to assess the growth viability of 12Z cells. (B) Scratch assay was performed to detect the migration capacity of 12Z cells (scale bar: 200 μm). (C) A Transwell invasion assay was used to analyze the invasion capacity of 12Z cells (scale bar: 200 μm). Data represent mean ± SD from three independent experiments. ***: P < 0.001, compared with control exosomes; ## : P < 0.01, ### : P < 0.001, compared with EMs exosomes.

Article Snippet: Collect the 12Z cells after co‐culture and seed them into the upper chamber of a Transwell insert precoated with matrix gel (HY‐K6001, MCE, USA).

Techniques: Cell Culture, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Invasion Assay, Control