Journal: European Journal of Medical Research

Article Title: Endothelial cell iron overload and ferroptosis mediate thrombosis and inflammation through the miR-32-5p/neurofibromin 2 pathway

doi: 10.1186/s40001-025-02716-y

Figure Lengend Snippet: Ferroptosis was induced after the serum of the TAO patient’s treatment. a The cell viability of HUVEC cells after FBS, control serum, or TAO serum treatment (n = 5). b mRNA levels of key iron and ferroptosis-related markers after treatment with serum from control or TAO patients (n = 3). c Cell viability evaluated the capability of DFO and Fer1 after TAO serum treatment (n = 5). Protein levels of key iron, ferroptosis-related markers d , and NF2-YAP pathway e after treatment with serum from control or TAO patients (n = 3). f Then, the cellular iron and lipid peroxidation levels after serum treatment. *showed compassion between the control group and disease groups (n = 3). T test or one-way ANOVA, *: P < 0.05; **: P < 0.01, ***: P < 0.001, Error bar means SEM

Article Snippet: For the Sham group, 100 μL saline was injected into the femoral artery, and all other operations were injected with sodium laurate to conduct the TAO model. For treatment, all rats received deferoxamine (DFO, 100 mg/kg, Shanghai, Sigma), Ferrostatin-1 (Fer1, 5 mg/kg, MedChemExpress, Shanghai, China), or saline intraperitoneally every two days after surgery.

Techniques: Control

Journal: European Journal of Medical Research

Article Title: Endothelial cell iron overload and ferroptosis mediate thrombosis and inflammation through the miR-32-5p/neurofibromin 2 pathway

doi: 10.1186/s40001-025-02716-y

Figure Lengend Snippet: A high level of exosomal miR-32-5p was involved in ferroptosis by binding to NF2. a , b Interaction of prediction between miR-32-5p and NF2. c The level of miR-32-5p in serum-derived exosomes from 15 controls and 15 TAO patients. d Luciferase activities of NF2 after miR-32-5p mimic transfection. e mRNA and f protein level of NF2 after miR-32-5p transfection (n = 3). g mRNA level of TFR1, ACSL4 after miR-32-5p transfection (n = 3). h Intracellular Fe 2+ and lipid peroxidation levels in HUVEC cells after miR-32-5p transfection, DFO, or Fer1 treatment (n = 3). T test or one-way ANOVA, *showed compassion between the control group and the disease groups. *: P < 0.05; **: P < 0.01, ***: P < 0.001, Error bar means SEM

Article Snippet: For the Sham group, 100 μL saline was injected into the femoral artery, and all other operations were injected with sodium laurate to conduct the TAO model. For treatment, all rats received deferoxamine (DFO, 100 mg/kg, Shanghai, Sigma), Ferrostatin-1 (Fer1, 5 mg/kg, MedChemExpress, Shanghai, China), or saline intraperitoneally every two days after surgery.

Techniques: Binding Assay, Derivative Assay, Luciferase, Transfection, Control

Journal: European Journal of Medical Research

Article Title: Endothelial cell iron overload and ferroptosis mediate thrombosis and inflammation through the miR-32-5p/neurofibromin 2 pathway

doi: 10.1186/s40001-025-02716-y

Figure Lengend Snippet: DFO and Fer1 improved clinical symptoms, inflammation, and thrombosis in the rat TAO model. a The schedule of rat TAO model introduction and treatments. DFO and Fer1 were treated from 7 days before sodium laurate injection to the endpoint of the model. b The curve of rat weight during the TAO process (n = 7). c The level of relative blood flow of the femoral arteries in different groups (n = 7). d The representative images of blood flow and tissue gangrene on the 14 th day after sodium laurate injection (n = 3). Then the tissues were evaluated by (E) HE (100 ×) and (F) Masson staining (100 ×) of femoral arteries in the rat TAO model, the black arrows showed infiltration of red cells and lymphocytes. n = 7. T test or one-way ANOVA, * showed compassion between the control group and the disease groups. *: P < 0.05; **: P < 0.01, ***: P < 0.001, Error bar means SEM

Article Snippet: For the Sham group, 100 μL saline was injected into the femoral artery, and all other operations were injected with sodium laurate to conduct the TAO model. For treatment, all rats received deferoxamine (DFO, 100 mg/kg, Shanghai, Sigma), Ferrostatin-1 (Fer1, 5 mg/kg, MedChemExpress, Shanghai, China), or saline intraperitoneally every two days after surgery.

Techniques: Injection, Staining, Control

Journal: European Journal of Medical Research

Article Title: Endothelial cell iron overload and ferroptosis mediate thrombosis and inflammation through the miR-32-5p/neurofibromin 2 pathway

doi: 10.1186/s40001-025-02716-y

Figure Lengend Snippet: DFO and Fer1 balanced iron metabolism and inhibited ferroptosis in the rat TAO model. a Iron levels of femoral arterial lesions in the rat TAO model (n = 7). b The mRNA level of iron related genes in femoral arterial lesions of the rat TAO model (n = 7). c Perls’ stain (200 ×) in arterial tissues (n = 7). d The MDA level of femoral arteries among different groups (n = 7). e TEM images showed mitochondrial morphology in tissues (n = 3). Finally, the levels of f MDA and 4HNE were measured. n = 7. one-way ANOVA, * showed compassion between the control group and the disease groups. *: P < 0.05; **: P < 0.01, ***: P < 0.001, Error bar means SEM

Article Snippet: For the Sham group, 100 μL saline was injected into the femoral artery, and all other operations were injected with sodium laurate to conduct the TAO model. For treatment, all rats received deferoxamine (DFO, 100 mg/kg, Shanghai, Sigma), Ferrostatin-1 (Fer1, 5 mg/kg, MedChemExpress, Shanghai, China), or saline intraperitoneally every two days after surgery.

Techniques: Staining, Control