HY-108659 Search Results


p2y1r antagonist mrs2500  (MedChemExpress)
91
MedChemExpress p2y1r antagonist mrs2500
A) Characterization of cultured primary myoepithelial cells after 5 days of culture. aSMA and KRT5 are markers for myoepithelial cells. B) Representative images of primary myoepithelial cells (KRT5+, red) stained with P2Y1 receptor antibody (gray). C) Representative Fura-2 ratiometric Ca 2+ images of primary myoepithelial cells before and after ATP. D) Summary of ratiometric Ca 2+ imaging (R/R 0 , R = 340/380) in primary myoepithelial cells. Cells were pre-incubated with or without P2Y1 receptor antagonist <t>MRS2500</t> (5 μM). Dashed line indicates the time when ATP (1, 10, 100 μM) was added. n = 3 pigs. E, F) ATP stimulates primary myoepithelial cell contraction (presented as ratio of total area after treatment). Panel E , effects of 5-HT, CGRP, or ATP on primary myoepithelial cell contraction. Panel F , P2Y1 receptor antagonist MRS2500 abolished ATP controlled primary myoepithelial cell contraction. Each data point represents the mean of imaged cells from a pig; p values are shown in graph; n.s, not significant; *, p < 0.05; one sample t test for panel E , paired Student’s t test for panel F . G) WT and CF SMG contraction with ATP (1 mM) or succinate (10 mM) treatment ex vivo . Each data point is the mean of 3 SMGs from an individual pig. P values are shown in graph, paired Student’s t test. H) Model of SMG PNEC sensing succinate to trigger myoepithelial contraction. PNECs located in airway SMGs express SUCNR1, the receptor for succinate. Under hypoxia, infection, and inflammation, succinate is generated by macrophages, other epithelial cells, or microorganisms in airway surface liquid, which diffuses into the SMGs. SUCNR1 on SMG PNEC responds to extracellular succinate by releasing ATP to the basolateral environment. ATP activates P2Y1 receptors on myoepithelial cells to cause Ca 2+ -dependent myoepithelial contraction. See also .
P2y1r Antagonist Mrs2500, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p2y1r antagonist mrs2500/product/MedChemExpress
Average 91 stars, based on 1 article reviews
p2y1r antagonist mrs2500 - by Bioz Stars, 2026-02
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ly2183240  (MedChemExpress)
91
MedChemExpress ly2183240
A) Characterization of cultured primary myoepithelial cells after 5 days of culture. aSMA and KRT5 are markers for myoepithelial cells. B) Representative images of primary myoepithelial cells (KRT5+, red) stained with P2Y1 receptor antibody (gray). C) Representative Fura-2 ratiometric Ca 2+ images of primary myoepithelial cells before and after ATP. D) Summary of ratiometric Ca 2+ imaging (R/R 0 , R = 340/380) in primary myoepithelial cells. Cells were pre-incubated with or without P2Y1 receptor antagonist <t>MRS2500</t> (5 μM). Dashed line indicates the time when ATP (1, 10, 100 μM) was added. n = 3 pigs. E, F) ATP stimulates primary myoepithelial cell contraction (presented as ratio of total area after treatment). Panel E , effects of 5-HT, CGRP, or ATP on primary myoepithelial cell contraction. Panel F , P2Y1 receptor antagonist MRS2500 abolished ATP controlled primary myoepithelial cell contraction. Each data point represents the mean of imaged cells from a pig; p values are shown in graph; n.s, not significant; *, p < 0.05; one sample t test for panel E , paired Student’s t test for panel F . G) WT and CF SMG contraction with ATP (1 mM) or succinate (10 mM) treatment ex vivo . Each data point is the mean of 3 SMGs from an individual pig. P values are shown in graph, paired Student’s t test. H) Model of SMG PNEC sensing succinate to trigger myoepithelial contraction. PNECs located in airway SMGs express SUCNR1, the receptor for succinate. Under hypoxia, infection, and inflammation, succinate is generated by macrophages, other epithelial cells, or microorganisms in airway surface liquid, which diffuses into the SMGs. SUCNR1 on SMG PNEC responds to extracellular succinate by releasing ATP to the basolateral environment. ATP activates P2Y1 receptors on myoepithelial cells to cause Ca 2+ -dependent myoepithelial contraction. See also .
Ly2183240, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ly2183240/product/MedChemExpress
Average 91 stars, based on 1 article reviews
ly2183240 - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier

Image Search Results


A) Characterization of cultured primary myoepithelial cells after 5 days of culture. aSMA and KRT5 are markers for myoepithelial cells. B) Representative images of primary myoepithelial cells (KRT5+, red) stained with P2Y1 receptor antibody (gray). C) Representative Fura-2 ratiometric Ca 2+ images of primary myoepithelial cells before and after ATP. D) Summary of ratiometric Ca 2+ imaging (R/R 0 , R = 340/380) in primary myoepithelial cells. Cells were pre-incubated with or without P2Y1 receptor antagonist MRS2500 (5 μM). Dashed line indicates the time when ATP (1, 10, 100 μM) was added. n = 3 pigs. E, F) ATP stimulates primary myoepithelial cell contraction (presented as ratio of total area after treatment). Panel E , effects of 5-HT, CGRP, or ATP on primary myoepithelial cell contraction. Panel F , P2Y1 receptor antagonist MRS2500 abolished ATP controlled primary myoepithelial cell contraction. Each data point represents the mean of imaged cells from a pig; p values are shown in graph; n.s, not significant; *, p < 0.05; one sample t test for panel E , paired Student’s t test for panel F . G) WT and CF SMG contraction with ATP (1 mM) or succinate (10 mM) treatment ex vivo . Each data point is the mean of 3 SMGs from an individual pig. P values are shown in graph, paired Student’s t test. H) Model of SMG PNEC sensing succinate to trigger myoepithelial contraction. PNECs located in airway SMGs express SUCNR1, the receptor for succinate. Under hypoxia, infection, and inflammation, succinate is generated by macrophages, other epithelial cells, or microorganisms in airway surface liquid, which diffuses into the SMGs. SUCNR1 on SMG PNEC responds to extracellular succinate by releasing ATP to the basolateral environment. ATP activates P2Y1 receptors on myoepithelial cells to cause Ca 2+ -dependent myoepithelial contraction. See also .

Journal: Developmental cell

Article Title: Pulmonary Neuroendocrine Cells Sense Succinate to Stimulate Myoepithelial Cell Contraction

doi: 10.1016/j.devcel.2022.08.010

Figure Lengend Snippet: A) Characterization of cultured primary myoepithelial cells after 5 days of culture. aSMA and KRT5 are markers for myoepithelial cells. B) Representative images of primary myoepithelial cells (KRT5+, red) stained with P2Y1 receptor antibody (gray). C) Representative Fura-2 ratiometric Ca 2+ images of primary myoepithelial cells before and after ATP. D) Summary of ratiometric Ca 2+ imaging (R/R 0 , R = 340/380) in primary myoepithelial cells. Cells were pre-incubated with or without P2Y1 receptor antagonist MRS2500 (5 μM). Dashed line indicates the time when ATP (1, 10, 100 μM) was added. n = 3 pigs. E, F) ATP stimulates primary myoepithelial cell contraction (presented as ratio of total area after treatment). Panel E , effects of 5-HT, CGRP, or ATP on primary myoepithelial cell contraction. Panel F , P2Y1 receptor antagonist MRS2500 abolished ATP controlled primary myoepithelial cell contraction. Each data point represents the mean of imaged cells from a pig; p values are shown in graph; n.s, not significant; *, p < 0.05; one sample t test for panel E , paired Student’s t test for panel F . G) WT and CF SMG contraction with ATP (1 mM) or succinate (10 mM) treatment ex vivo . Each data point is the mean of 3 SMGs from an individual pig. P values are shown in graph, paired Student’s t test. H) Model of SMG PNEC sensing succinate to trigger myoepithelial contraction. PNECs located in airway SMGs express SUCNR1, the receptor for succinate. Under hypoxia, infection, and inflammation, succinate is generated by macrophages, other epithelial cells, or microorganisms in airway surface liquid, which diffuses into the SMGs. SUCNR1 on SMG PNEC responds to extracellular succinate by releasing ATP to the basolateral environment. ATP activates P2Y1 receptors on myoepithelial cells to cause Ca 2+ -dependent myoepithelial contraction. See also .

Article Snippet: Submerged tissues were incubated with or without SUCNR1 antagonist NF-56-EJ40 (MedChem Express, HY-130246) and P2Y1R antagonist MRS2500 (Tocris, 2159) for ~30 minutes, respectively.

Techniques: Cell Culture, Staining, Imaging, Incubation, Ex Vivo, Infection, Generated

KEY RESOURCES TABLE

Journal: Developmental cell

Article Title: Pulmonary Neuroendocrine Cells Sense Succinate to Stimulate Myoepithelial Cell Contraction

doi: 10.1016/j.devcel.2022.08.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Submerged tissues were incubated with or without SUCNR1 antagonist NF-56-EJ40 (MedChem Express, HY-130246) and P2Y1R antagonist MRS2500 (Tocris, 2159) for ~30 minutes, respectively.

Techniques: Virus, Recombinant, Plasmid Preparation, Software