Journal: iScience

Article Title: The role of m6A modification in the risk prediction and Notch1 pathway of Alzheimer’s disease

doi: 10.1016/j.isci.2024.110235

Figure Lengend Snippet:

Article Snippet: Sodium valproate , MCE , HY10585A.

Techniques: Recombinant, Methylation, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Transgenic Assay, Software

Journal: Journal of Immunology Research

Article Title: ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells

doi: 10.1155/2020/4384696

Figure Lengend Snippet: Cultured mouse microglial cells (BV2) expressed Iba1 and CGRP receptor components CRLR, RAMP1, or CRCP. (a) Expression of Iba1 (a marker of microglia, red) and its colocalization with CRLR, RAMP1, or CRCP staining (green) in cultured microglial cells. Scale bar 40 μ m. (b) Western blot analysis of HDAC2 expression in microglia with treatment with CGRP at 0, 1, 2, 4, and 6 h, respectively. Relative amounts of proteins were calculated by normalizing to GAPDH. Data are presented as the mean ± SEM ( N = 4 independent cell culture preparations, Mann-Whitney U tests or Kruskal-Wallis tests). ∗ P < 0.05.

Article Snippet: To assess the possible impact of HDAC2 on the expression of iNOS, RIG-I, and NF- κ B induced by CGRP, 0.5 mmol/L valproic acid (VPA, HDAC2 inhibitor, MedChemExpress, Cat. #1069-66-5) were preapplied for 60 min and coapplied together with CGRP for 2 h at 37°C.

Techniques: Cell Culture, Expressing, Marker, Staining, Western Blot, MANN-WHITNEY

Journal: Journal of Immunology Research

Article Title: ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells

doi: 10.1155/2020/4384696

Figure Lengend Snippet: Effect of CGRP on the enriched region (peak) distribution of the ChIP-seq reads of HDAC2 in microglia treated with CGRP compared with the control. (a) Genome-wide distribution of enrichments relative to annotated genes. (b) The peak distribution of ChIP-seq reads of HDAC2 in control and microglial cells treated with CGRP. (c) The distribution of HDAC2 peaks on promoters relative to gene transcription start sites (TSSs). Shown are HDAC2 peak frequencies relative to the distance from the nearest annotated TSS in microglial cells treated with CGRP.

Article Snippet: To assess the possible impact of HDAC2 on the expression of iNOS, RIG-I, and NF- κ B induced by CGRP, 0.5 mmol/L valproic acid (VPA, HDAC2 inhibitor, MedChemExpress, Cat. #1069-66-5) were preapplied for 60 min and coapplied together with CGRP for 2 h at 37°C.

Techniques: ChIP-sequencing, Genome Wide

Journal: Journal of Immunology Research

Article Title: ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells

doi: 10.1155/2020/4384696

Figure Lengend Snippet: The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of genes with differentially enriched HDAC2 in microglial cells treated with CGRP. GO annotation of upregulated genes (a) or downregulated genes (b) with HDAC2 enrichments of the CGRP treatment group versus the control. Bar plots show the top ten fold enrichment values of the significant enrichment terms involving biological process (BP), cellular component (CC), and molecular function (MF). (c) KEGG pathway analysis of genes with differentially enriched HDAC2 in microglial cells treated with CGRP. The bar plot shows the top ten fold enrichment values of the significant enrichment pathway. Analysis by DAVID and KOBAS online tools ( http://www.geneontology .org and http://www.genome.jp/kegg ).

Article Snippet: To assess the possible impact of HDAC2 on the expression of iNOS, RIG-I, and NF- κ B induced by CGRP, 0.5 mmol/L valproic acid (VPA, HDAC2 inhibitor, MedChemExpress, Cat. #1069-66-5) were preapplied for 60 min and coapplied together with CGRP for 2 h at 37°C.

Techniques:

Journal: Journal of Immunology Research

Article Title: ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells

doi: 10.1155/2020/4384696

Figure Lengend Snippet: CGRP evokes increases in the expression of iNOS, RIG-I, and NF- κ B in microglial cells in a time-dependent manner. (a) Western blot analysis of iNOS, RIG-I, and NF- κ B expression in microglial cells with treatment of CGRP at 0, 1, 2, 4, and 6 h, respectively. (b) Western blotting analyses for the effect of HDAC2 inhibitor VPA on CGRP-evoked iNOS, RIG-I, and NF- κ B protein expression in microglial cells with treatment of CGRP and pretreatment with VPA. Relative amounts of proteins were calculated by normalizing to GAPDH. All values are expressed as the means ± SEMs ( N = 4 independent cell culture preparations, Mann-Whitney U tests or Kruskal-Wallis tests). ∗ P < 0.05 versus controls; # P < 0.05 versus CGRP only groups.

Article Snippet: To assess the possible impact of HDAC2 on the expression of iNOS, RIG-I, and NF- κ B induced by CGRP, 0.5 mmol/L valproic acid (VPA, HDAC2 inhibitor, MedChemExpress, Cat. #1069-66-5) were preapplied for 60 min and coapplied together with CGRP for 2 h at 37°C.

Techniques: Expressing, Western Blot, Cell Culture, MANN-WHITNEY